Iron chloride hexahydrate

Manufactured by Merck Group

Sourced in Germany, United States

Iron chloride hexahydrate is an inorganic compound with the chemical formula FeCl3·6H2O. It is a crystalline solid that is soluble in water and other polar solvents. Iron chloride hexahydrate is commonly used as a laboratory reagent and in various industrial processes.

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Iron(III) chloride hexahydrate (437 citations) Iron chloride (54 citations) Iron(III) chloride hexahydrate (FeCl3·6H2O), (46 citations) Iron (II) chloride hexahydrate (8 citations) Iron chloride hexahydrate (FeCl3•6H2O) (2 citations) Iron(III) chloride hexahydrate and iron(II) chloride tetrahydrate (2 citations)

41 protocols using iron chloride hexahydrate

Antioxidant Capacity Evaluation Methods

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Folin–Ciocalteu reagent, DPPH, ABTS, TPTZ, potassium persulfate, gallic acid, Trolox and the other standards were purchased from Sigma-Aldrich (St. Louis, MO, USA). LC-MS grade m ethanol and water, glacial acetic acid, iron chloride hexahydrate, sodium carbonate and ethanol for the extraction were purchased from Merck KGaA (Darmstadt, Germany). Vanillic acid (≥97%), rutin (≥95%), catechin (≥99%), chlorogenic acid (≥95%), ferulic acid (≥99 %), cyanidin-3-O-glucoside (Cy-3-glu) (≥98%), ascorbic acid (>99%), quercetin (≥95%) and geraniol (≥98.5%) standards were also purchased from Sigma-Aldrich (St. Louis, MO, USA).

Gil-Martínez L., Mut-Salud N., Ruiz-García J.A., Falcón-Piñeiro A., Maijó-Ferré M., Baños A., De la Torre-Ramírez J.M., Guillamón E., Verardo V, & Gómez-Caravaca A.M. (2023). Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits. Foods, 12(7), 1505.

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Quantification of Phenolic Compounds

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Gallic acid hydrate was from Panreac (Barcelona, Spain). Glycine (99.5%) was from Applichem (Darmstadt, Germany). Iron chloride hexahydrate was from Merck (Darmstadt, Germany). L-Lactic acid, ascorbic acid (99.5%), sodium acetate trihydrate, sodium carbonate anhydrous (99%) and aluminum chloride anhydrous (98%) were from Penta (Praha, Czechia). Chlorogenic acid (95%) was from Fluorochem (Hadfield, UK). NeoChlorogenic acid (≥98%) was from Merck (Darmstadt, Germany). Folin-Ciocalteu reagent, rutin (quercetin 3-O-rutinoside) hydrate (>94%), isorhamnetin 3-O-glucoside and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were from Sigma-Aldrich (St. Louis, MO, USA).

Kaltsa O., Lakka A., Grigorakis S., Karageorgou I., Batra G., Bozinou E., Lalas S, & Makris D.P. (2020). A Green Extraction Process for Polyphenols from Elderberry (Sambucus nigra) Flowers Using Deep Eutectic Solvent and Ultrasound-Assisted Pretreatment. Molecules, 25(4), 921.

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Antioxidant Compounds Characterization

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Glycine (99.5%) was from Applichem (Darmstadt, Germany). Iron chloride hexahydrate was from Merck (Darmstadt, Germany). Rutin (quercetin 3-O-rutinoside) hydrate, kaempferol 3-O-glucoside, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), 2,2-diphenylpicrylhydrazyl (DPPH) and Folin-Ciocalteu reagent were from Sigma-Aldrich (St. Louis, MO, USA). L-Lactic acid, sodium carbonate anhydrous (99%), ascorbic acid (99.5%), and sodium acetate trihydrate and aluminium chloride anhydrous (98%) were from Penta (Praha, Czechia). Gallic acid hydrate was from Panreac (Barcelona, Spain). Pelargonin (pelargonidin 3,5-di-O-glucoside) chloride was from Extrasynthese (Genay, France).

Lakka A., Grigorakis S., Karageorgou I., Batra G., Kaltsa O., Bozinou E., Lalas S, & Makris D.P. (2019). Saffron Processing Wastes as a Bioresource of High-Value Added Compounds: Development of a Green Extraction Process for Polyphenol Recovery Using a Natural Deep Eutectic Solvent. Antioxidants, 8(12), 586.

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Phytochemical Compound Characterization

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Neo Chlorogenic acid (≥98%) was from Merck (Darmstadt, Germany). Chlorogenic acid (95%) was from Fluorochem (Hadfield, UK). Ferulic acid, caffeic acid, Folin-Ciocalteu reagent, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), hydroxypropyl β-cyclodextrin and 2,2-diphenylpicrylhydrazyl (DPPH) were from Sigma-Aldrich (Darmstadt, Germany). Sodium hydroxide and iron chloride hexahydrate was from Merck (Darmstadt, Germany). Sodium carbonate anhydrous (99%), aluminum chloride anhydrous (98%) and ethanol (99.8%) were from Penta (Praha, Czechia). All solvents used for chromatography were HPLC grade.

Lakka A., Lalas S.I., & Makris D.P. (2020). Development of a Low-Temperature and High-Performance Green Extraction Process for the Recovery of Polyphenolic Phytochemicals from Waste Potato Peels Using Hydroxypropyl β-Cyclodextrin. Applied Sciences, 10(10), 3611-3611.

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Synthesis of Iron-Magnesium Hydroxide Compounds

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Magnesium chloride hexahydrate (MgCl₂·6H₂O), iron chloride hexahydrate (FeCl₃·6H₂O), ammonium hydroxide solution, triethanolamine (TEA, C₆H₁₅NO₃), acetylene black, and polytetrafluoroethylene (PTFE) were purchased from Sigma-Aldrich. Potassium hydroxide (KOH, 99.99%) and ethanol absolute (EtOH) were purchased from Panreac. All chemicals were used as received. Milli-Q water was obtained from Millipore Milli-Q equipment.

Seijas-Da Silva A., Oestreicher V., Coronado E, & Abellán G. (2022). Influence of Fe-clustering on the water oxidation performance of two-dimensional layered double hydroxides. Dalton Transactions (Cambridge, England : 2003), 51(12), 4675-4684.

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Synthesis of Colloidal Nanoparticles

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Copper(I)
acetate (CuOAc, 97%), sodium oleate
(97%), and hexane (anhydrous, 95%) were purchased from TCI. Trioctylamine
(TOA, 98%), oleylamine (OLAM, 70%), 1-Octadecene (ODE, 90%), oleic
acid (OLAC, 90%), iron chloride hexahydrate (FeCl₃, 98%),
tetradecylphosphonic acid (TDPA, 97%), ethanol (anhydrous), cyclohexane
(anhydrous), and n-docosane (99%) were purchased
from Sigma-Aldrich and used as received.

Loiudice A., Niau B.P, & Buonsanti R. (2022). Crystal-Phase Control of Ternary Metal Oxides by Solid-State Synthesis with Nanocrystals. ACS Nanoscience Au, 2(3), 233-238.

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Evaluating Lipid Peroxidation Biomarkers

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7-DHC, ferric citrate, iron chloride hexahydrate,
ferric ammonium citrate, sulfate heptahydrate, a-tocopherol, deferoxamine
mesylate salt, pentanal, hexanal, 2-trans-heptenal,
2-trans-octenal, 2-trans-nonenal,
2,4-trans,trans-hexadienal, 2,4-trans,trans-nonadienal, and 2,4-trans,trans-decadienal were
obtained from Sigma-Aldrich. Retinol, 2,4-dinitrophenylhydrazine (DNPH),
and LpDNPH S10L cartridges were purchased from Thermo Fisher Scientific.
Retinal, coenzyme Q₁₀, and vitamin D₃ were purchased
from Chem-Impex Int’l Inc. Oleic acid (OA), linoleic acid (LA),
9,12,15-cis,cis,cis-octadecatrienoic acid (NLA 18:3),
arachidonic acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic
acid (DHA), and 10,12-trans,cis-octadecadienoic acid
(CLA 18:2) were purchased from Nu-Chek Prep, Inc. 9,11,13-trans,trans,trans-octadecatrienoic acid (CLA 18:3) was obtained
from Sapphire North America. 1,2-dimyristoyl-sn-glycero-3-phosphocholine
(DMPC) was obtained from Avanti Polar Lipids. Imidazole ketone erastin
(IKE), RSL3, liproxstatin-1, and ferrostatin-1 were obtained from
MedChemExpress. 4-hydroxynonenal (4-HNE) was purchased from Cayman
Chemical. The radical initiator, MeOAMVN, was purchased from Wako
Chemicals.

Do Q., Zhang R., Hooper G, & Xu L. (2023). Differential Contributions of Distinct Free Radical Peroxidation Mechanisms to the Induction of Ferroptosis. JACS Au, 3(4), 1100-1117.

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Synthesis and Characterization of Dye-Doped Iron Oxide Nanoparticles

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Dopamine hydrochloride (≥98%), iron chloride hexahydrate (FeCl₃⋅6H₂O), iron chloride tetrahydrate (FeCl₂·4H₂O), ammonium hydroxide (NH₄OH), and ammonium persulfate ((NH₄)₂S₂O₈ (APS)) (≥98%) were purchased from Sigma Aldrich (Burghausen, Germany). Acetic acid (CH₃COOH) (≥99.8%) was obtained from Honeywell Fluka (Seelze, Germany). Tris-(hydroxymethyl)-aminomethane buffer (≥99.5%), Erythrosine B (ERT-B) (C₂₀H₈I₄Na₂O₅) (≥80%) and Patent blue V (VF) (C₂₇H₃₁N₂NaO₇S₂) were purchased from Loba Chemie (Maharashtra, India). All other chemicals used were of analytical grade and were used without further purification.

Elfadil D., Della Pelle F., Compagnone D, & Amine A. (2022). Green Synthesis of Molecularly Imprinted Polymers for Dispersive Magnetic Solid-Phase Extraction of Erythrosine B Associated with Smartphone Detection in Food Samples. Materials, 15(21), 7653.

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Comprehensive Phytochemical Analysis of Fruits

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ABTS (2,2′-azinobis-[3-ethylbenzthiazoline-6-sulfonic acid] diammonium salt), Trolox (6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), catechin, Folin and Ciocalteu’s phenol reagent, sodium carbonate (Na₂CO₃), gallic acid, vanillin, catechin, sodium nitrite (NaNO₂), aluminum chloride hexahydrate (AlCl₃ × 6 H₂O), DTT (dithiothreitol), NEM (N-ethylmaleimide), 2,2′-dipyridyl, iron chloride hexahydrate (FeCl₃ x 6 H₂O), ascorbic acid, as well as all HPLC-grade solvents were purchased from Sigma–Aldrich (Milan, Italy). High purity standards for the qualitative-quantitative determination of fatty acids (palmitic, stearic, oleic, linoleic, punicic), organic acid (citric, malic, succinic), sugars (glucose and fructose) were purchased from Sigma–Aldrich (Milan, Italy). Anthocyanin (cyaniding 3-glucoside, cyaniding 3,5-diglucoside, delphinidin 3-glucoside, delphinidin 3,5-diglucoside) standards were purchased from Extrasynthase (GenayCedex, France).

Montefusco A., Durante M., Migoni D., De Caroli M., Ilahy R., Pék Z., Helyes L., Fanizzi F.P., Mita G., Piro G, & Lenucci M.S. (2021). Analysis of the Phytochemical Composition of Pomegranate Fruit Juices, Peels and Kernels: A Comparative Study on Four Cultivars Grown in Southern Italy. Plants, 10(11), 2521.

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Polyphenol Extraction from PDL Leaves

Cited 1 time

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In this study, the following chemicals: iron chloride hexahydrate (FeCl₃·6H₂O 98%), sodium hydroxide (NaOH), ethanol (CH₃CH₂OH 99.9%), methanol (CH₃OH 99.9%), gallic acid (C₇H₆O₅), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and dimethyl sulfoxide anhydrous (DMSO) (C₂H₆SO 99.9%) were all supplied by Sigma Aldrich (Darmstadt, Germany) and used. The experiments were performed employing only analytical-grade chemicals and reagents.
Polyphenols were extracted from PDL, which was collected from Seville (Spain), following a previous protocol [41 (link),42 (link)]. Briefly, leaves were dried for 44 days at 22 ± 3 °C and 35% RH. Polyphenols were extracted by the Soxhlet method with distilled water (water: leaf powder ratio 10:1) for 8 h. The extract had a total polyphenol content (TPC) of 39 ± 2 mg GAE/g extract.

Abdullah J.A., Jiménez-Rosado M., Guerrero A, & Romero A. (2023). Effect of Calcination Temperature and Time on the Synthesis of Iron Oxide Nanoparticles: Green vs. Chemical Method. Materials, 16(5), 1798.

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