Celltiter 96 aqueous one solution cell proliferation assay kit

Manufactured by Promega

Sourced in United States, China, Japan, United Kingdom, France, Italy

The CellTiter 96® AQueous One Solution Cell Proliferation Assay kit is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The assay is based on the chemical reduction of a tetrazolium compound, resulting in the production of a colored formazan product that is quantified using a spectrophotometer.

Automatically generated - may contain errors

Lab products found in correlation

Microplate reader, by Agilent Technologies (17 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (14 mentions) Synergy 2, by Agilent Technologies (10 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (10 mentions) Trypsin edta, by Thermo Fisher Scientific (9 mentions) Infinite m200, by Tecan (8 mentions) Spectramax m5, by Molecular Devices (8 mentions) Microplate reader, by Bio-Rad (7 mentions) Multiskan mk3 microplate reader, by Thermo Fisher Scientific (7 mentions) Protease inhibitor cocktail, by Merck Group (7 mentions) Wallac 1420 multilabel counter, by PerkinElmer (6 mentions) Spectramax m2, by Molecular Devices (6 mentions) Multiskan mk3, by Thermo Fisher Scientific (6 mentions) B27 supplement, by Thermo Fisher Scientific (6 mentions) U0126, by Merck Group (6 mentions) Neurobasal media, by Thermo Fisher Scientific (6 mentions) Multiskan fc, by Thermo Fisher Scientific (6 mentions) Microplate reader, by Thermo Fisher Scientific (6 mentions) Spectramax 190, by Molecular Devices (5 mentions) Akt inhibitor x, by Santa Cruz Biotechnology (5 mentions)

783 protocols using celltiter 96 aqueous one solution cell proliferation assay kit

Cytotoxicity of ASD, KRG, and Rg3 in Bronchial Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human bronchial epithelial BEAS-2B cells, transformed by adenovirus, were purchased from American Type Culture Collection (Rockville, MD, USA). Epithelial cells were cultured with DMEM/F12 medium supplemented with 100 international units of penicillin, 100 μg/mL streptomycin, 2 μg/mL of amphotericin B, and heat-inactivated 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) at 37 °C and 5% CO₂. Cell suspensions at 5 × 104 cells/well were grown to 80% confluence for further studies.
To determine the cytotoxic effects of ASD, BEAS-2B cells were incubated with 0, 10, 100, or 500 μg/mL of ASD for 72 h. Cell cytotoxicity was determined using a CellTiter-96^® aqueous one solution cell proliferation assay kit (Promega, Madison, WI, USA). For this assay, tetrazolium compound and phenazine etho-sulfate were added to each well and incubated for 4 h at 37 °C in a 5% CO₂ chamber. Color intensities were assessed using a microplate reader at wavelength of 490 nm. The cytotoxic effects of KRG (0 to 500 μg/mL) and Rg3 (0 to 1000 μg/mL) were also determined using a CellTiter-96^® aqueous one solution cell proliferation assay kit (Promega).

Shin S.H., Ye M.K., Lee D.W., Kang B.J, & Chae M.H. (2021). Effect of Korean Red Ginseng and Rg3 on Asian Sand Dust-Induced MUC5AC, MUC5B, and MUC8 Expression in Bronchial Epithelial Cells. Molecules, 26(7), 2002.

+ Open protocol

+ Expand

Evaluating AR-Targeted Prostate Cancer Therapies

Check if the same lab product or an alternative is used in the 5 most similar protocols

To measure AR-mediated cell proliferation, 10,000 VCaP cells (AR positive prostate cancer cells) were plated in each well of a 96-well plate and incubated in steroid-free assay medium for 12 hours. After adherence, VCaP cells were treated with increasing concentrations of Zeta55, MDV3100 or SAHA together with 10 nM DHT for AR antagonist assays or without DHT for AR agonist assays. After 7 days, cell viability was measured using a CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega). 8,000 LNCaP cells, 5,000 DU145 cells (AR negative prostate cancer cells) or HEK293 cells (AR negative noncancerous cells) per well were cultured in DMEM with 10% FBS for 12 hours. After treatment with increasing concentrations of Zeta55, MDV3100 or SAHA for 3 days, the cell viability was measured using a CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega).

Zhou M., Zheng H., Li Y., Huang H., Min X., Dai S., Zhou W., Chen Z., Xu G, & Chen Y. (2021). Discovery of a novel AR/HDAC6 dual inhibitor for prostate cancer treatment. Aging (Albany NY), 13(5), 6982-6998.

+ Open protocol

+ Expand

FACS Analysis of Cd-Induced Apoptosis and Cell Cycle

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescence-activated cell sorting (FACS) analysis was done to test the effect of Cd exposure on cell cycle distribution and apoptosis of all cell lines. Equal numbers of cells were seeded and cultured for 24 hours. Cells were then harvested, washed with cold PBS, and stained with propidium iodide (PI-A) and Annexin-V-FITC using FITC-ANNEXIN V-PI-A KIT (BD Biosciences) for apoptosis and nuclear stain 4′,6-diamidino-2-phenylindole (DAPI) (BD Biosciences) for cell cycle analysis according to the manufacturer’s protocol. Stained cells were immediately analyzed by FACS (BD FACSVerse; BD Biosciences). Cell viability/proliferation was determined by using the CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega, Madison, WI) according to the manufacturer’s protocol. For cell viability assay, cells were seeded in 96-well microplates at a density of 5×10³ cells per well. After 24 hours of culture, cell viability was determined by adding 20ul/well of the CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega, Madison, WI) according to the manufacturer’s protocol. Absorbance at 490 nm was measured with a kinetic microplate reader (Spectra MAX 190; Molecular Devices Co., Sunnyvale, CA).

Dasgupta P., Kulkarni P., Bhat N.S., Majid S., Shiina M., Shahryari V., Yamamura S., Tanaka Y., Gupta R.K., Dahiya R, & Hashimoto Y. (2020). Activation of the Erk/MAPK signaling pathway is a driver for cadmium induced prostate cancer.. Toxicology and applied pharmacology, 401, 115102.

+ Open protocol

+ Expand

Cell Proliferation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded at 1 × 10⁴ cells/well in 96-well plates in 200 μL medium and left to adhere overnight. The following afternoon, media was replaced with media containing 0.5% FCS and incubated overnight once more. After each timepoint, cell proliferation assays were carried out using the Promega CellTiter 96^® Aqueous One Solution Cell Proliferation Assay kit (Promega, Southampton, UK) following the manufacturer’s instructions. Spent media was removed from all wells, followed by addition of 100 μL of 0.5% FCS-containing media and 20 μL of CellTiter 96^® Aqueous One Solution Reagent. The plate was then incubated at 37 °C with 5% CO₂ for 4 h. Absorbance was read at 490nm using a GloMax^® Discover Microplate Reader (Promega, UK).

Davis A.M., Rapley A., Dawson C.W., Young L.S, & Morris M.A. (2021). The EBV-Encoded Oncoprotein, LMP1, Recruits and Transforms Fibroblasts via an ERK-MAPK-Dependent Mechanism. Pathogens, 10(8), 982.

+ Open protocol

+ Expand

MTS Assay for Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

For cell viability analysis, an MTS assay was performed using the Promega CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega Corporation, Wisconsin, USA) based on the manufacturer's instructions. Briefly, 50 μl of MTS reagent was added to the respective Ramos lymphoma cell medium (500 μl) and incubated for about 4 hr at 37°C in a 5% CO₂ in air atmosphere. Absorbance reading at 490 nm using a spectrophotometer ELISA reader was taken (mQuant; BioTek, Winooski, VT, USA).

Lin H.D., Fong C.Y., Biswas A, & Bongso A. (2020). Hypoxic Wharton's Jelly Stem Cell Conditioned Medium Induces Immunogenic Cell Death in Lymphoma Cells. Stem Cells International, 2020, 4670948.

+ Open protocol

+ Expand

Cell Proliferation Assay with Glutamine

Check if the same lab product or an alternative is used in the 5 most similar protocols

We typically plated 0.5 million cells per 10-cm dish in duplicate in culture medium without glutamine and with dialyzed fetal bovine serum (glutamine-free medium), with glutamine and with dialyzed fetal bovine serum (glutamine-containing medium), and with glutamine and regular fetal bovine serum (complete medium). We added the test compounds at the same time as the trypsinized cells were plated on the dishes. We examined the cultures under microscope, and stained with crystal violet when appropriate. If a treatment had an effect, we waited for the surviving cells to yield colonies (approximately 3 weeks) before staining and photographing or scanning stained dishes. To determine whether a test compound had any effect on cell growth in actively proliferating cells, we performed a similar experiment in a 96-well format and determined relative cell proliferation with MTS assay using CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega Corporation, Madison, WI).

Singh B., Kinne H.E., Milligan R.D., Washburn L.J., Olsen M, & Lucci A. (2016). Important Role of FTO in the Survival of Rare Panresistant Triple-Negative Inflammatory Breast Cancer Cells Facing a Severe Metabolic Challenge. PLoS ONE, 11(7), e0159072.

+ Open protocol

+ Expand

Cervical Cancer Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cervical cancer cells (5 × 10³ per well) were seeded in 96-well plates and transfected on the following day. Cell proliferation was determined at 24, 48, 72, and 96 h using the CellTiter 96 AQueous One Solution Cell Proliferation Assay Kit (Promega), according to the manufacturer's instructions as we previously described [12 (link)]. The assays were performed in triplicate and were repeated three times.

Wang N., Li Y, & Zhou J. (2017). miR-31 Functions as an Oncomir Which Promotes Epithelial-Mesenchymal Transition via Regulating BAP1 in Cervical Cancer. BioMed Research International, 2017, 6361420.

+ Open protocol

+ Expand

Cell Proliferation Assay in Tet-Inducible SH-SY5Y Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation was measured using the Cell-Titer 96® AQueous One Solution Cell Proliferation Assay kit (MTS, Promega #G3582). Tet off-inducible FLAG-TRIP12 SH-SY5Y cells (1×10⁴ cells/well) were seeded into 96-well plates in triplicate in presence or absence of doxycycline (DOX). MTS solution (20 μl) was added to each well at 0, 12, 24, 48 h and, after two hours incubation, the cell proliferation rate was measured at 490nm.

Seo B.A., Kim D., Hwang H., Kim M.S., Ma S.X., Kwon S.H., Kweon S.H., Wang H., Yoo J.M., Choi S., Kwon S.H., Kang S.U., Kam T.I., Kim K., Karuppagounder S.S., Kang B.G., Lee S., Park H., Kim S., Yan W., Li Y.S., Kuo S.H., Redding-Ochoa J., Pletnikova O., Troncoso J.C., Lee G., Mao X., Dawson V.L., Dawson T.M, & Ko H.S. (2021). TRIP12 Ubiquitination of Glucocerebrosidase Contributes to Neurodegeneration in Parkinson’s Disease. Neuron, 109(23), 3758-3774.e11.

+ Open protocol

+ Expand

Comparing NF1+ and NF1KD Cell Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols

To compare cell growth between NF1⁺ and NF1^KD cells, cells stably expressing NF1 shRNA were first seeded in estrogen-deprived medium, typically in triplicate, with DOX added at the same time. Two days later (Day-0), the medium was replaced with a fresh medium containing either vehicle control or compound(s) under investigation, with medium change every three days thereafter. The number of viable cells was measured using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit (Promega) six days later (Day-6). Cell growth between parental and NF1 knock-out cells was similarly examined except no DOX was added. Apoptosis was measured by FITC-Annexin (Becton Dickinson) staining followed by FACS.

Zheng Z.Y., Anurag M., Lei J.T., Cao J., Singh P., Peng J., Kennedy H., Nguyen N.C., Chen Y., Lavere P., Li J., Du X.H., Cakar B., Song W., Kim B.J., Shi J., Seker S., Chan D.W., Zhao G.Q., Chen X., Banks K.C., Lanman R.B., Shafaee M.N., Zhang X.H., Vasaikar S., Zhang B., Hilsenbeck S.G., Li W., Foulds C.E., Ellis M.J, & Chang E.C. (2020). Neurofibromin is an Estrogen Receptor-α Transcriptional Co-repressor in Breast Cancer. Cancer cell, 37(3), 387-402.e7.

+ Open protocol

+ Expand

Cell Viability Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were transfected as described above in 96-well plates, then 24 and 48 h p.t. the viability of the cells in transfected and non-transfected cultures was determined using a CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega, USA). Briefly, the medium in the wells of 96-well plates was replaced with 100 μl of PBS, and 20 μl aliquots of the CellTiter 96 reagent were added per well. The absorbance was recorded at 490 nm with the Multiskan Ascent plate reader (ThermoFisher Scientific, USA) immediately after the CellTiter 96 reagent addition and after 1 h of incubation at 37 °C in humidified atmosphere with 5% CO₂.

Komissarov A., Karaseva M., Roschina M., Kostrov S, & Demidyuk I. (2022). The SARS-CoV-2 main protease doesn’t induce cell death in human cells in vitro. PLoS ONE, 17(5), e0266015.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!