Anti gata6 af1700

Manufactured by R&D Systems

Anti-GATA6 (AF1700) is a laboratory reagent produced by R&D Systems. It is an antibody that recognizes and binds to the GATA6 protein, which is a transcription factor involved in the regulation of gene expression. The core function of this product is to enable the detection and study of the GATA6 protein in biological samples.

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Lab products found in correlation

Anti actin, by Cell Signaling Technology (1 mentions) Dynabeads protein g, by Thermo Fisher Scientific (1 mentions) Glutathione sepharose 4b beads, by GE Healthcare (1 mentions) Anti dicer ab14601, by Abcam (1 mentions) Anti gapdh ac002, by ABclonal (1 mentions)

Spelling variants of this product

AF1700 (18 citations) GATA6 (13 citations) Anti-GATA6 (10 citations)

2 protocols using anti gata6 af1700

Investigating Metabolic Proteins in Cancer Cells

Cited 1 time

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Western blot was performed as described previously (Bu et al., 2015 (link)). Samples were prepared using the cancer cells purified by FACS based on mCherry expression. Antibodies used included anti-human ALDOB antibody (PA5–30218, 1:2000, Pierce), anti-Hexokinase (C35C4, 1:1000, Cell Signaling), anti-ketohexokinase (ab197593, 1:2000, Abcam), anti-GATA6 (AF1700, 1:1000, R&D system) and anti-actin (13E5, 1:1000, Cell Signaling; 4967S, Cell Signaling Technology).

Bu P., Chen K.Y., Xiang K., Johnson C., Crown S.B., Rakhilin N., Ai Y., Wang L., Xi R., Astapova I., Han Y., Li J., Barth B.B., Lu M., Gao Z., Mines R., Zhang L., Herman M., Hsu D., Zhang G.F, & Shen X. (2018). Aldolase B Mediated Fructose Metabolism Drives Metabolic Reprogramming of Colon Cancer Liver Metastasis. Cell metabolism, 27(6), 1249-1262.e4.

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MLL4-Complex Protein Purification

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Anti-Vimentin antibody (V6630) was purchased from Sigma-Aldrich, anti-N-cadherin (610920), anti-E-cadherin (610181), anti-MLL3 (53641), anti-α-catenin (610193), and anti-GATA3 (5852) from Cell Signaling Technology, anti-GAPDH (AC002) from ABclonal Technology, anti-UTX (A302-374A), anti-PA1 (A301-978A), anti-WDR5 (A302-429A), anti-PTIP (A300-370A), anti-ASH2L (A300-489A), and anti-RBBP5 (A300-109A) from Bethyl, anti-Dicer (ab14601) from Abcam, anti-MLL4 (C15310100) from Diagenode, anti-GATA1 (60011-1-Ig) and anti-GATA4 (19530-1-AP) from Proteintech, anti-GATA2 (sc-267) and anti-GATA5 (sc-373683) from Santa Cruz, and anti-GATA6 (AF1700) from R&D Systems. Dynabeads Protein G was obtained from Invitrogen (Thermo Fisher Scientific) and Glutathione-Sepharose 4B beads from GE Healthcare Bio-Sciences. shRNAs were synthesized by GenePharma Co. Ltd. (Shanghai, China). Glutathione S-transferase (GST)-MLL4-complex plasmids were created by inserting full-length MLL4-complex components into the pGEX-4T-3 expression vector as described previously^{21 (link)}.

Yu W., Huang W., Yang Y., Qiu R., Zeng Y., Hou Y., Sun G., Shi H., Leng S., Feng D., Chen Y., Wang S., Teng X., Yu H, & Wang Y. (2019). GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer. Cell Death & Disease, 10(11), 832.

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