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Ventana dp200 scanner

Manufactured by Roche
Sourced in United States

The Ventana DP200 scanner is a digital pathology slide scanning device designed for use in clinical and research laboratories. The core function of the Ventana DP200 scanner is to capture high-resolution digital images of tissue samples mounted on microscope slides.

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3 protocols using ventana dp200 scanner

1

Galectin-9 Immunohistochemistry Protocol

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Paraffin blocks were sectioned (5 μm) and placed on poly-L-lysine-coated glass slides. The sections were deparaffinized for 1 h at 60°C. To block the activity of endogenous peroxidase, the sections were incubated in methanol containing 0.3% H2O2 for 30 min. Thereafter, every step was followed by three washes in PBS buffer. To block nonspecific antibody binding, slides were incubated for 1 h in 1% BSA in PBS. Sections were incubated with galectin-9 antibody (ab69630, Abcam, Cambridge, MA USA) diluted at 1:400 overnight at 4°C. Then, the sections were incubated with HRP-conjugated anti-rabbit IgG (ab6721, Abcam) for 1 h at room temperature. Staining was visualised by incubating the slides with DAB substrate (ImmPACT DAB Peroxidase substrate, Vector SK-4105, Burlingame, CA USA) according to the manufacturer’s instructions. To distinguish the nuclei, sections were counterstained in haematoxylin solution (HX87960674 Merck, Darmstadt Germany) for 10 sec. Images were obtained using a VENTANA DP200 scanner (Roche Diagnostics, Tucson, AZ USA). The intensity of galectin-9 expression was determined using Image-Pro Image Analysis Software (Media Cybernetics, Rockville, MD USA). The mean density was calculated for each sample and the expression of galectin-9 was reported in lumens.
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2

Tumor Morphology Evaluation and Categorization

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Hematoxylin and eosin stained formalin fixed paraffine embedded tissue sections of 142 tumors for which corresponding RNAseq data was available were evaluated independently by two pathologists (M.R., M.C.H.). All slides were digitized using a Ventana DP200 scanner (Roche) and tumor morphologies were assessed and categorized into adenocarcinoma, adenocarcinoma with cribriform morphology, high-grade carcinoma not otherwise specified (NOS), high-grade neuroendocrine carcinoma, carcinoid like tumor, hybrid tumor with neuroendocrine and adenocarcinoma features, high-grade carcinoma with pleomorphic giant cells and high-grade carcinoma with squamoid features. Morphology calls were integrated and in cases of discrepancies, slides were re-reviewed to achieve a consensus.
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3

Histopathological Evaluation of Tumor Morphologies

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Hematoxylin and eosin–stained formalin-fixed paraffine-embedded tissue sections of 142 tumors for which corresponding RNAseq data were available were evaluated independently by two pathologists (M.P. Roudier and M.C. Haffner). All slides were digitized using a Ventana DP200 scanner (Roche) and tumor morphologies were assessed and categorized into adenocarcinoma, adenocarcinoma with cribriform morphology, high-grade carcinoma not otherwise specified (NOS), high-grade neuroendocrine carcinoma, carcinoid like tumor, hybrid tumor with neuroendocrine and adenocarcinoma features, high-grade carcinoma with pleomorphic giant cells and high-grade carcinoma with squamoid features. Morphology calls were integrated and in cases of discrepancies, slides were re-reviewed to achieve a consensus.
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