Cd11c clone bu15

Manufactured by BioLegend

CD11c (clone Bu15) is a mouse monoclonal antibody that binds to the CD11c antigen, which is expressed on the surface of dendritic cells and other myeloid cells. CD11c is a component of the integrin family of cell surface receptors and plays a role in cell adhesion and migration.

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2 protocols using cd11c clone bu15

Isolation and Phenotyping of Human ILCs

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Lung cells from HSPC-engrafted MISTRG mice were isolated as described above. Cell surface staining with fluorescent antibodies was performed as previously described [17 (link), 18 (link)]. The following antibodies were used for flow cytometry: CD127 (clone A019D5, Biolegend), CRTH2 (clone BM16, Biolegend), CD94 (clone DX22, Biolegend), CXCR4 (clone 12G5, Biolegend), CXCR6 (clone K041E5, Biolegend), GITR (clone 108-17, Biolegend), CD16 (clone 3G8, Biolegend), CD69 (clone FN50, Biolegend), CD117 (104D2D1, Beckman Coulter), HLA-DR (clone G46-6, BD Biosciences), CD2 (clone RPA-2.10, BD Biosciences), CD56 (clone NCAM16.2, BD Biosciences), CD81 (clone JS-81, BD Biosciences), CD94 (clone HP-3D9, BD Biosciences), CD98 (clone UM7F8, BD Biosciences), CD103 (clone Ber-ACT8, BD Biosciences), and CD45RA (clone HI100, BD Biosciences). In all experiments, ILCs were defined as human CD45⁺Lin^-CD3⁻TCRαβ⁻CD127⁺CD94⁻ cells. Lineage markers included CD14 (clone M5E2, Biolegend), CD19 (clone HIB19, Biolegend), CD11c (clone Bu15, Biolegend), CD123 (clone 6H6, Biolegend), FcεRIα (clone AER-37 (CRA-1), Biolegend), CD34 (clone 581, BD Biosciences), TCRαβ (clone IP26) and CD3 (clone SK7).

Alisjahbana A., Mohammad I., Gao Y., Evren E, & Willinger T. (2023). Single-cell RNA sequencing of human lung innate lymphoid cells in the vascular and tissue niche reveals molecular features of tissue adaptation. Discovery Immunology, 2(1), kyad007.

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Quantifying Costimulatory Molecule Expression on APCs

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Enriched APCs were stained with antibodies recognizing OX40L (clone ANC10G1; Ancell), ICOSL (clone 136726; R&D Bio-techne), or CD86 (clone FUN-1; R&D Bio-techne), or isotype control, and HLA-DR (clone LN3; eBiosciences), CD14 (clone 61D3; eBiosciences), CD11c (clone Bu15; BioLegend), CD1c (clone L161; BioLegend), CD304 (clone 12C2; BioLegend), and CD141 (clone AD5-14H12; Miltenyi Biotec). Samples were acquired on a FACS Verse instrument (BD Biosciences). Stain index was calculated to quantify the level of expression as follows: stain index = (MFI_sample − MFI_isotype)/(2 × SD_isotype), where MFI indicates mean fluorescence intensity, and SD indicates standard deviation.

Durand M., Walter T., Pirnay T., Naessens T., Gueguen P., Goudot C., Lameiras S., Chang Q., Talaei N., Ornatsky O., Vassilevskaia T., Baulande S., Amigorena S, & Segura E. (2019). Human lymphoid organ cDC2 and macrophages play complementary roles in T follicular helper responses. The Journal of Experimental Medicine, 216(7), 1561-1581.

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