Glass bottomed petri dish

Manufactured by MatTek

Sourced in Morocco

The glass-bottomed Petri dish is a laboratory equipment used for culturing and observing cells or tissues. It consists of a shallow cylindrical dish made of glass with a transparent glass bottom, allowing for visual inspection and imaging of the contents under a microscope.

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Lab products found in correlation

Plan neofluar, by MatTek (2 mentions) Spinning disc csu x1, by Zeiss (2 mentions) Plan apochromat, by Zeiss (2 mentions) Ms 222, by Merck Group (2 mentions) No 1.5 glass coverslip, by MatTek (2 mentions) Axio zoom v16, by Zeiss (2 mentions) Lsm 780, by Zeiss (2 mentions) Axio imager z2, by Zeiss (1 mentions) Ultrapure low melting point agarose, by Thermo Fisher Scientific (1 mentions) Illustrator 2022, by Adobe (1 mentions) Thermanox, by Thermo Fisher Scientific (1 mentions) Lsm 510 confocal scanning microscope, by Zeiss (1 mentions) A1r confocal microscope, by Nikon (1 mentions) Norland optical adhesive 81, by Norland Products (1 mentions) Low melting point agarose, by Merck Group (1 mentions) Tcs sp8 upright microscope, by Leica (1 mentions) Lignocaine, by AstraZeneca (1 mentions)

Close spelling variants of this product

Glass-bottomed Petri dishes Glass cover slip-bottomed 50 mm petri dish with 30 mm glass diameter Glass coverslip-bottomed 50-mm Petri dish Glass-bottomed dishes Glass-bottomed dish Petri dishes Petri dish

11 protocols using glass bottomed petri dish

Confocal Time-lapse Imaging of Insect Embryos

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Confocal time-lapse imaging of Tribolium embryos injected with GAP43-YFP mRNA was performed as previously described (Benton et al., 2013 (link); Benton, 2018 (link)) at 25–32°C at time intervals from 2 to 10 min between timepoints using 20x, 40x, or 63x objectives. For live imaging of the posterior poles, eggs were propped up vertically (resting against another egg for stability) on a glass-bottomed Petri dish (MatTek) with their posterior against the glass. Live imaging transgenic nuclear GFP (nGFP) (Sarrazin et al., 2012 (link)) or LifeAct-GFP (van Drongelen et al., 2018 (link)) embryos was done at room temperature using the Zeiss AxioImager.Z2 in combination with an Apotome.2 and movable stage (Zen2 Blue).
For imaging Gryllus bimaculatus embryos we used a Zeiss AxioZoom.V16, equipped with a movable stage. Gryllus embryos were placed on 1.5% agarose and were covered with Voltalef H10S oil (Sigma). Imaging was performed at 25–27°C.

Benton M.A., Frey N., Nunes da Fonseca R., von Levetzow C., Stappert D., Hakeemi M.S., Conrads K.H., Pechmann M., Panfilio K.A., Lynch J.A, & Roth S. (2019). Fog signaling has diverse roles in epithelial morphogenesis in insects. eLife, 8, e47346.

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Imaging Zebrafish Trunk and Brain Vasculature

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A Zeiss LSM 700 confocal microscope was used for live imaging of the trunk and brain vasculature. Embryos were anaesthetized with a low dose of tricaine, placed in a glass-bottomed Petri dish (MatTek) on a layer of 1.2% low melt agarose and imaged using Plan-Apochromat 10×/0.45 and LCI Plan-Neofluar 25×/0.8 objectives.
In wt embryos, at 60 hpf, an average of 13 CtAs have connected to the basilar artery (BA)46 (link).
vegfaa^{promoter-less} larvae were obtained from F2 heterozygous parents generated by outcrossing the founder and F1 fish.
To measure blood flow velocity, we performed time-lapse imaging of the trunk region of 78 hpf wild-type, maternal zygotic hbegfa^Δ7 -/- and maternal zygotic hbegfa^{full locus del.} -/- larvae using a Zeiss Spinning disc CSU-X1 confocal microscope with a high-speed camera, and quantified blood flow velocity in the dorsal aorta by measuring the time needed by erythrocytes to move 200 μm at the level of the fifth and sixth somites using Zen Blue as previously described47 (link).
hbegfa^{full locus del.} larvae were obtained from F2 homozygous parents generated by outcrossing the founder.
hbegfa^Δ7 and vegfaa^Δ10 larvae were obtained from an incross of F3 heterozygous parents that were generated by consecutive outcrosses.

El-Brolosy M.A., Kontarakis Z., Rossi A., Kuenne C., Günther S., Fukuda N., Kikhi K., Boezio G.L., Takacs C., Lai S.L., Fukuda R., Gerri C., Giraldez A.J, & Stainier D.Y. (2019). Genetic compensation triggered by mutant mRNA degradation. Nature, 568(7751), 193-197.

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Fluorescence Imaging Procedures for C. elegans and Zebrafish

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Fluorescence images of C. elegans were acquired using a Zeiss LSM 700 confocal microscope (Plan-Apochromat 10×/0.45 objective lens). Worms were mounted immobilized in polystyrene microbeads as described (71 (link)). Fluorescence images of zebrafish embryos were acquired using a Zeiss LSM 700 confocal microscope (Plan-Apochromat 10×/0.45 objective lens). Embryos were mounted in 1% UltraPure Low Melting Point Agarose (Thermo Fisher Scientific) in egg water with tricaine in a glass-bottomed petri dish (MatTek). Obtained images were subsequently processed with the ZEN software (black edition). All figures were prepared using Adobe Photoshop 2022 and Adobe Illustrator 2022.

Jiang Z., El-Brolosy M.A., Serobyan V., Welker J.M., Retzer N., Dooley C.M., Jakutis G., Juan T., Fukuda N., Maischein H.M., Balciunas D, & Stainier D.Y. (2022). Parental mutations influence wild-type offspring via transcriptional adaptation. Science Advances, 8(47), eabj2029.

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Quantifying Candida parapsilosis Biofilms

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C. parapsilosis biofilms were grown on Thermanox (Nunc) slides in in 6 well plates. Briefly, overnight cultures were washed twice in PBS and diluted to an A₆₀₀ of 1 in SD media with 50 mM glucose. 5 ml was added to each well, and incubated for 2 h at 37°C at 50 rpm. The slides were gently removed and placed into a fresh well containing 5 ml of PBS, and gently placed into a well containing fresh SD/50 mM glucose media and incubated for 48 h at 37°C at 50 rpm. The slides were removed and washed as above, and then stained with 25 µg ml⁻¹ concanavalin A (conA)-Alexa Fluor 594 conjugate (C-11253; Bio-science) for 45 min at 37°C. The liquid was removed from each well, and the Thermonox slides were flipped and placed on a 35-mm-diameter glass-bottomed petri dish (MatTek Corp., Ashland, MA). The biofilms were observed with a Zeiss LSM510 confocal scanning microscope with a ×40-magnification oil objective. A HeNe1 laser was used to excite at a 543-nm wavelength. All images were captured and analyzed using a Zeiss LSM Image Browser and Fiji.

Holland L.M., Schröder M.S., Turner S.A., Taff H., Andes D., Grózer Z., Gácser A., Ames L., Haynes K., Higgins D.G, & Butler G. (2014). Comparative Phenotypic Analysis of the Major Fungal Pathogens Candida parapsilosis and Candida albicans. PLoS Pathogens, 10(9), e1004365.

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Cellular Uptake of Functionalized Rho-NPs

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LS174T cells were seeded in a 35 mm glass bottomed Petri dish (MatTek). After 3 days, the cells were incubated with Rho-NPs (Rho-NP-pD, Rho-NP-pD-ZWC_0.1, Rho-NP-pD-ZWC_0.3, and Rho-NP-PEG) at pH 6.7 or 7.6 for 4 h and washed twice with fresh complete medium to remove free or loosely bound NPs. The cells were incubated in 2 μg/mL Hoechst for 5 min for nuclei staining and washed twice with medium. Live cells were imaged with a Nikon A1R confocal microscope (Nikon America Inc., Melville, NY).

Park J., Pei Y., Hyun H., Castanares M.A., Collins D.S, & Yeo Y. (2017). Small molecule delivery to solid tumors with chitosan-coated PLGA particles: A lesson learned from comparative imaging. Journal of controlled release : official journal of the Controlled Release Society, 268, 407-415.

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Time-lapse Imaging of Zebrafish Embryos

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Embryos with beads in the desired location were anesthetized using MS-222 (Sigma Aldrich, A5040) in E2 solution and dorsally mounted on a glass-bottomed petridish (MatTek) in 1% low melting agarose prepared in E2 solution (Fig. 4a). Time-lapse images were acquired using an inverted confocal microscope (Zeiss LSM780) using a 40x water immersion objective (NA 1.2) with laser lines 488 nm for eGFP and 561 nm for Cy3. Images were analyzed using the open source software FIJI^{43 (link)}.

Träber N., Uhlmann K., Girardo S., Kesavan G., Wagner K., Friedrichs J., Goswami R., Bai K., Brand M., Werner C., Balzani D, & Guck J. (2019). Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development. Scientific Reports, 9, 17031.

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Imaging Zebrafish Trunk and Brain Vasculature

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Hydrogel Fabrication in Custom Chambers

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Hydrogels were made in custom sample chambers in a glass-bottomed Petri dish (d = 35 mm, no. 1.5 glass coverslip, MatTek Corporation). A polydimethylsiloxane (PDMS, Sylgard) cylindrical chamber with a 10 mm outer diameter and 8 mm inner diameter was made using biopsy punches (Integra Biosciences). The PDMS chamber was then loosely attached to the bottom of the glass-bottomed Petri dish using ultraviolet (UV) curing adhesive (Norland Optical Adhesive 81, Norland Products Inc.), which was cured with UV light at 365 nm for 3 min. This adhesive allows for suﬃcient attachment to the Petri dish but also allows for removal of the PDMS chamber. The PDMS chamber was used to hold the polymer precursor solution during the gelation process and was removed following gelation to enable equal swelling (in growth medium) in the axial and radial directions.

Mazzeo M.S., Chai T., Daviran M, & Schultz K.M. (2018). Characterization of the Kinetics and Mechanism of Degradation of Human Mesenchymal Stem Cell-Laden Poly(ethylene glycol) Hydrogels. ACS applied bio materials, 2(1), 81-92.

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Zebrafish Embryo Anesthesia and Imaging

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Embryos were anaesthetised in 0.014% tricaine (MS-222, Sigma-Aldrich), mounted in a 35 mm glass-bottomed petri dish (0.17 mm, MatTek) using 0.6-1% low melting point agarose (Sigma-Aldrich) containing 0.014% tricaine, and bathed in Danieau's buffer containing 0.007 (0.5×) to 0.014% (1×) tricaine and 0.003% PTU (as indicated). Time-lapse imaging was performed using a Leica TCS SP8 upright microscope with a Leica HCX IRAPO L ×25/0.95 water-dipping objective and heating chamber, or on an upright 3i spinning-disc confocal using a Zeiss Plan-Apochromat 20×, 40× or 63×/1.0 NA water-dipping objective. Image processing was performed using Fiji software (Schindelin et al., 2012 (link)).

Geudens I., Coxam B., Alt S., Gebala V., Vion A.C., Meier K., Rosa A, & Gerhardt H. (2019). Artery-vein specification in the zebrafish trunk is pre-patterned by heterogeneous Notch activity and balanced by flow-mediated fine-tuning. Development (Cambridge, England), 146(16), dev181024.

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Anesthesia and Preparation for Dunnart Imaging

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Joeys were imaged between P20-P40. Mothers were anaesthetized and joeys were exposed as described above. Joeys were carefully removed from the teat by gently pulling, whilst squeezing the base of the teat with forceps. The joey was then restrained by wrapping its body in gauze and encasing it within a modified 1.5 μL centrifuge tube (Eppendorf, Hamburg), leaving the head exposed. Joeys were then anesthetized on ice for 4–6 minutes, or until unresponsive to stimuli. Topical local anesthesia (1% lignocaine; AstraZeneca, Cambridge) was applied to the skin on the scalp with a fine paintbrush, and the scalp covering the electroporated area was then removed. It was also critical to carefully remove the muscles superficial to the skull in order to prevent contractions that moved the sample during calcium imaging. Finally, a glass-bottomed petri dish (MatTek, MA) was fixed to the skull of the dunnart joey with cyanoacrylate glue (UHU, Bühl). The animal was then allowed to recover from ice anesthesia whilst the cyanoacrylate set for at least 30 mins.

Suárez R., Bluett T., McCullough M.H., Avitan L., Black D.A., Paolino A., Fenlon L.R., Goodhill G.J, & Richards L.J. (2023). Cortical activity emerges in region-specific patterns during early brain development. bioRxiv.

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