The colony morphologies of M. purpureus LQ-6 and Δ5162 on PDA plates were monitored using a digital camera (Canon EOS 80 D, Tokyo, Japan), and the colony diameters were measured daily. Mycelial pellets were monitored using a digital camera (Canon EOS 80 D), and free hyphae were monitored using a biological microscope (Olympus, Tokyo, Japan). Samples of M. purpureus LQ-6 and Δ5162 were treated according to a previously described method (Liu, Chai et al., 2019), and images were observed using a transmission electron microscope (TEM) (Hitachi H-7650, Hitachi, Japan).
Eos 80d
Manufactured by Canon
Sourced in Japan, China
The EOS 80D is a high-performance digital SLR camera from Canon. It features a 24.2-megapixel APS-C CMOS sensor, DIGIC 6 image processor, and a 45-point all cross-type AF system. The camera is capable of capturing high-quality still images and Full HD 1080p video.
Automatically generated - may contain errors
Lab products found in correlation
Dmc6200, by Leica (2 mentions)
H 7650, by Hitachi (1 mentions)
Quanta 250 feg sem, by Thermo Fisher Scientific (1 mentions)
Supra 55, by Zeiss (1 mentions)
Proline xc, by Planmeca (1 mentions)
Macro ring lite mr 14 exii, by Canon (1 mentions)
Ckx41, by Olympus (1 mentions)
Dm2500, by Leica (1 mentions)
Mp e 65 mm objective, by Canon (1 mentions)
M205 fca, by Leica (1 mentions)
S 4800, by Hitachi (1 mentions)
Escalab 250 xi xps system, by Thermo Fisher Scientific (1 mentions)
High vacuum grease, by Dow (1 mentions)
B 100ap r, by Analytik Jena (1 mentions)
Gelatin filter no 15, by Kodak (1 mentions)
Matlabr2018b software, by MathWorks (1 mentions)
Originpro 2018b, by OriginLab (1 mentions)
Zwickiline z1, by Zwick Roell (1 mentions)
Axio scope a1, by Zeiss (1 mentions)
Dm4000b, by Leica (1 mentions)
40 protocols using eos 80d
1
Characterization of M. purpureus Strains
2
Cryo-fractured Surface Morphology Analysis
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Macrophotographical imaging was performed using a Canon EOS 80D equipped with a Sigma 18–250 mm macro objective (both Canon Inc., Japan). SEM imaging of cryo-fractured surface morphology was performed with a SEM QUANTA FEG 250 (FEI Deutschland GmbH, Germany) operating in low vacuum with an accelerating voltage of 5 kV, using a secondary electron detector (LVD). Samples were flash-frozen with liquid nitrogen and manually fractured using pincers. To enhance visibility of morphological characteristics, one half of each fractured sample was chemically etched using 1 M NaOH for 72 h, rinsed with distilled water and dried under ambient conditions, whereas the other side of the fracture edge acted as direct reference sample. The specimens were fixed onto aluminum trays with conductive carbon adhesive pads and the aluminum trays were mounted on custom 90° angle brackets to orient fracture edges toward the electron beam source.
3
Measuring Birefringence Profile of Starch Films
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A customized
experimental setup using a photoelastic modulator (PEM) was used to
measure the retardation of the circular starch film along its diameter
(see theSupporting Information for details).
The thickness profile of the film was measured by cutting it along
the same diameter after the retardation measurement and taking the
cross-sectional image by using a microscope (Olympus BX50) attached
with a digital camera (Canon EOS 80D). The digital image processing
software (imageJ, NIH) was used to obtain the thickness profiles of
the films. The linear birefringence profile of the film was calculated
from the ratio of the measured optical path difference and the corresponding
thickness.
experimental setup using a photoelastic modulator (PEM) was used to
measure the retardation of the circular starch film along its diameter
(see the
The thickness profile of the film was measured by cutting it along
the same diameter after the retardation measurement and taking the
cross-sectional image by using a microscope (Olympus BX50) attached
with a digital camera (Canon EOS 80D). The digital image processing
software (imageJ, NIH) was used to obtain the thickness profiles of
the films. The linear birefringence profile of the film was calculated
from the ratio of the measured optical path difference and the corresponding
thickness.
4
Photographic Documentation and Image Processing
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Photographs in Figs. 1 and 2 were taken with a digital reflex camera (Canon EOS 80D; Canon Inc., Ota, Tokyo, Japan) with the setting “close-up”. They were changed to black and white, cut, arranged, and labeled using the program Adobe Photoshop (Version CS4 Windows; Adobe Inc., San José, CA, USA). Figure 4 shows the result of an experiment by isoelectric focusing and immunoblot. The nitrocellulose membrane was scanned with a flatbed scanner (HP Scanjet G3110; HP Inc., Palo Alto, CA, USA) as color image with the resolution 1200 dpi (settings: highlights 20, shadows 0, midtones 0, gamma 1.0) and was saved as TIF-file. The area displayed in Fig. 4 was cut from the full-length image and annotated using the program Adobe Photoshop (Version CS4 Windows; Adobe Inc., San José, CA, USA). No adjustments were made to brightness or contrast. The full-length blot can be found in Supplementary Figure S1 .
5
Characterizing Membrane Microstructures
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The G, G-E, G-W, and G-E-W membranes were freeze dried, placed on a uniform platform, and photographed by a digital camera (Canon EOS 80D, Canon Co., Ltd, China) to observe the color, appearance, and other characteristics. The membranes were fixed on the sample table with a conductive adhesive, sprayed with 7 nm gold (20 mA, 90 s), and imaged by scanning electron microscopy (SEM, SUPRA55, ZEISS Co., Ltd, Germany) to observe the microstructure of the materials [43 (link)].
6
Standardized Dental Imaging Protocol
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The images were taken following the guidelines of the "American Academy of Cosmetic Dentistry" (AACD), following the modality "full smile frontal view, 1:2 (1:3) magnification and non-retracted view" (https://aacd. com. Accessed March 27, 2021) . In addition to the AACD criteria and to calibrate the images, an adhesive with standardized dimensions was placed on the lower lip of each participant, as a tab for scale.
The photographs were taken by an operator (S-R-L), in two rooms with participants in identical lightening. The subjects' heads were positioned with Frankfort plane parallel to the ground by using an orthopantomography device (Proline XC; Planmeca, Helsinki, Finland). An APS-C reflex camera (Canon EOS 80D; Canon Inc., Tokyo, Japan) equipped with a macro lens (Canon EF 100 mm f / 2.8 Macro USM; Canon Inc.) and a ring flash (Macro ring lite MR-14EXII; Canon Inc.) was used. The parameters used were: ISO: 200; shutter speed: 1/125; diaphragm aperture f: 20; and distance to the target: 0.49 m. Since the digital camera had an APS-C sensor (22.2 mm × 14.8 mm), a magnification ratio of 1:3 was applied to achieve the 1:2 magnification defined in the protocol of the AACD.
The photographs were taken by an operator (S-R-L), in two rooms with participants in identical lightening. The subjects' heads were positioned with Frankfort plane parallel to the ground by using an orthopantomography device (Proline XC; Planmeca, Helsinki, Finland). An APS-C reflex camera (Canon EOS 80D; Canon Inc., Tokyo, Japan) equipped with a macro lens (Canon EF 100 mm f / 2.8 Macro USM; Canon Inc.) and a ring flash (Macro ring lite MR-14EXII; Canon Inc.) was used. The parameters used were: ISO: 200; shutter speed: 1/125; diaphragm aperture f: 20; and distance to the target: 0.49 m. Since the digital camera had an APS-C sensor (22.2 mm × 14.8 mm), a magnification ratio of 1:3 was applied to achieve the 1:2 magnification defined in the protocol of the AACD.
7
Chikungunya Virus Infection Assay
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ICRES-CHIKV RNAs were produced and purified as described above. C2C12 cells were electroporated with ICRES-CHIKV RNAs (1 μg RNA was electroporated into 1.2 x106 cells) and incubated at 37°C. Cell supernatants were collected at 8, 24 and 48 hours post electroporation (h.p.e.), diluted with cell medium and applied to monolayers of BHK-21 cells for 1 h at 37°C. The inoculum was aspirated and plates were overlaid with 0.8% methylcellulose for 48 h at 37°C for plaque formation. For titration of intracellular viruses, cells were freeze/thawed 3 times and supernatants were collected by centrifugation at 12000×g for 10 min., prior to application to BHK-21 cells. All virus work was performed in a Biological Safety Level 3 (BSL3) laboratory. Plaques were visualised by photography with a Canon EOS 80D.
8
Histological Evaluation of Articular Cartilage
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For three days, MCs from joint cartilages were preserved in 4% buffered paraformaldehyde. After two weeks of decalcification in 0.5 M ethylene diamine tetra-acetate (EDTA) solution at four degrees Celsius, the samples were desiccated, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E). An optical microscope (CKX41 Olympus Corporation, Tokyo, Japan) and a digital camera (EOS 80D, Canon Inc., Tokyo, Japan) were used to evaluate the histologic sections. Five independent evaluators independently analyzed the severity of articular cartilage sections and scored them using the International Cartilage Repair Society (ICRS) histology grading method [30 (link)] (Table 3 ). A higher score demonstrated superior quality of the repaired tissue.
9
Microscopic Examination of Specimens
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Examination was with a compound microscope (Leica DM2500) equipped with differential interference contrast (DIC) and a digital SLR (Canon EOS 80D). Imaging was with a dry, 10x objective (brightfield). Polyvinyl alcohol (PVA) mounting medium was used to hold the specimens in a relatively still position. Specimens were imaged without a coverslip within minutes of being placed into the PVA. Contrast was heightened by setting the turret of the DIC between the 10 and 40 intervals.
10
Imaging Fish Caudal Fin Development
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For documentation of the caudal-fin development, embryos and larvae were photographed using a Leica M205 FCA with an attached Leica DMC6200 camera operated with the software Leica Application Suite (version: 3.6.0.20104). Additionally, specimens of Glossolepis incisus, Oryzias woworae and Poropanchax normani were imaged using fluorescent light making use of the autofluorescent properties of Alizarin red. Adult specimens were photographed using a Canon EOS 80D with a Canon MP-E 65 mm objective. Images were processed, without altering any morphological structures, and drawings were produced using Adobe Photoshop (version: 22.0.0). Figure plates were assembled in Adobe Illustrator (version: 25.0).
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