Mncl2 4h2o

Macronutrients used were NaNO₃ (PanReac, Barcelona, Spain) and NaH₂PO₄.2H₂O (Honeywell International Inc., Charlotte, NC, USA), whereas the trace elements used for media preparation were Na₂EDTA (Sigma-Aldrich, St. Louis, MO, USA), FeCl₃.6H₂O (Acros Organics, Geel, Belgium), CuSO₄.5H₂O (Sigma-Aldrich, St. Louis, MO, USA), ZnSO₄.7H₂O (Sigma-Aldrich, St. Louis, MO, USA), CoCl₂.6H₂O (Thermo Fisher Scientific, Pittsburg, PA, USA), MnCl₂.4H₂O (Acros Organics, Geel, Belgium) and Na₂MoO₄.2H₂O (Chem-Lab NV, Zedelgem, Belgium). Cyanocobalamin, Thiamine HCl and Biotin were purchased from Sigma-Aldrich, St. Louis, MO, USA.
Chemicals used for analysis were ammonium bicarbonate (Sigma-Aldrich, St. Louis, MO, USA), Folin–Ciocalteu reagent (Sigma-Aldrich, St. Louis, MO, USA), N,N′-dimethylformamide (DMF) (Honeywell International Inc., Charlotte, NC, USA), chlorophorm HPLC grade (Honeywell International Inc., Charlotte, NC, USA), mercury (III) oxide red (Sigma-Aldrich, St. Louis, MO, USA), 2,4,6-tri(2–pyridyl)–s–triazine (TPTZ) (Alfa Aesar, Ward Hill, MA, USA), water for analysis (Carlo Erba, Cornaredo, Milan, Italy), methanol HPLC grade (Thermo Fisher Scientific, Pittsburg, PA, USA), sodium acetate trihydrate (Merck, Kenilworth, NJ, USA), acetic acid (PENTA, Radiová, Prague, Czech Republic) and hydrochloric acid (HCl) (PENTA, Radiová, Prague, Czech Republic), EDTA-Na₂.

Powder samples of Mn²⁺ doped zeolite-Y (denoted as xMn/ zeolite-Y, x = 0.4, 0.8, 1.2 mol/L), Yb³⁺ doped zeolite-Y (denoted as yYb/zeolite-Y, y = 0.05, 0.1, 0.2, 0.4, 0.8 mol/L), and those codoped with both Mn²⁺ and Yb³⁺ (denoted as yYb³⁺, xMn²⁺/zeolite-Y, y = 0.2 mol/L, x = 0, 0.2, 0.4, 0.6, 0.8, 1.0 mol/L) were prepared by ion-exchange reaction method. The raw materials zeolite-Y (SiO₂/Al₂O₃ = 5.1), Yb(NO₃)₃·5H₂O (99.9%) and MnCl₂·4H₂O (99.99%) were purchased from Alfa Aesar. The zeolites were initially stirred in the aqueous solution of MnCl₂·4H₂O and Yb(NO₃)₃·5H₂O at 80 °C for 36 h. Then the resulting products were centrifugated and washed with deionized water. After several times of washing and centrifugation, the products were kept in the oven at 80 °C for 12 h for drying. Finally, they were placed into an alumina crucible and sintered at 800 °C for 1 h under a reductive atmosphere (5%H₂ + 95%N₂) in a furnace.

RbCl (99.99% metals basis), CdCl₂ (anhydrous, 99.99% metals basis), SnCl₂·2H₂O (99.99% metals basis), hypophosphorous acid (H₃PO₂, 50 wt% in H₂O), isopropanol (IPA, AR), and dimethylformamide (DMF, AR) were purchased from Aladdin. MnCl₂·4H₂O (99% metals basis) was purchased from Alfa Aesar. Hydrochloric acid (HCl, AR) was purchased from Guangzhou Chemical Reagent Factory. All chemicals were used as received without any further purification.

Overnight cultures were grown in 5 ml Luria broth media containing appropriate antibiotics. Antibiotic concentrations were as follows: kanamycin (50 μg ml⁻¹; IBI Scientific), ampicillin (200 μg ml⁻¹; Fisher BioReagents), tetracycline (20 μg ml⁻¹; Fisher BioReagents) and spectomycin (50 μg ml⁻¹). IBA production was carried out with M9P medium, consisting of M9 medium (33.7 mM Na₂HPO₄, 22 mM KH₂PO₄, 8.55 mM NaCl, 9.35 mM NH₄Cl, 1 mM MgSO₄ and 0.1 mM CaCl₂; BD Bacto); 5 g l⁻¹ yeast extract (BD Bacto); 50 g l⁻¹ glucose (Fisher BioReagents); and 1,000-fold dilution of A5 trace metal mix (2.86 g H₃BO₃ (Fisher Chemical), 1.81 g MnCl₂·4H₂O (Alfa Aesar), 0.079 g CuSO₄·5H₂O (Sigma-Aldrich) and 49.4 mg Co(NO₃)₂·6H₂O (Sigma-Aldrich) per liter water). Optical densities (OD) were measured at 600 nm with a Synergy H1 hybrid plate reader (BioTek Instruments, Inc.).