At the heading stage, the third internodes were cut into thin pieces and segments, then fixed in the fixative solution (75% ethanol, 5% acetic acid, 5% glycerol, and 5% formaldehyde) for at least 24 h, and then dehydrated through an ethanol series. For scanning electron microscopy, the samples were critical point-dried, sputter-coated with gold and observed with Gemini SEM300 scanning electron microscope (ZEISS, Germany). For lignin staining, the third internode of fresh barley stem was sliced by freehand, and then one to two drops of 5% phloroglucinol ethanol solution was applied to the material, followed by one drop of concentrated hydrochloric acid to soak the material, which was immediately observed under a light microscope and photographed.
Gemini sem 300 scanning electron microscope
Manufactured by Zeiss
Sourced in Germany
The Gemini SEM 300 is a scanning electron microscope (SEM) manufactured by Zeiss. It is designed to provide high-resolution imaging of samples by using a focused beam of electrons to scan the surface of the specimen. The Gemini SEM 300 can produce detailed images that reveal the topography and composition of the sample at the nanoscale level.
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Lab products found in correlation
E 1010 ion sputter, by Hitachi (1 mentions)
H 7650 transmission electron microscope, by Hitachi (1 mentions)
Hcp 2 critical point dryer, by Hitachi (1 mentions)
Talos l120c g2 transmission electron microscope, by Thermo Fisher Scientific (1 mentions)
Potassium ferricyanide, by Maclin Biochemical Technology (1 mentions)
Potassium ferrocyanide, by Maclin Biochemical Technology (1 mentions)
Milli q ultrapure water system, by Merck Group (1 mentions)
Aflatoxin b1, by Pribolab (1 mentions)
Aflatoxin g1, by Pribolab (1 mentions)
Aflatoxin g2, by Pribolab (1 mentions)
Jem 2800 electron microscope, by JEOL (1 mentions)
X ray photoelectron spectrometer, by JEOL (1 mentions)
Labx xrd 6100 x ray diffractometer, by Shimadzu (1 mentions)
6 protocols using gemini sem 300 scanning electron microscope
1
Lignin Staining and SEM Analysis of Barley Stem
2
Microscopic Analysis of Fungal Interactions
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Blocks of 7-day-old fungal mycelia were taken from the edge of Fusarium graminearum mycelia grown on PDA alone or confrontation with P. peoriae RP51 and prepared for electron microscopy. The blocks were immersed in 2.5% (v/v) glutaraldehyde in 0.1 M phosphate buffer (pH 7.0) for 4 h, washed with the phosphate buffer for 15 min three times, then immersed in 1% (w/v) OsO4 in the phosphate buffer for 2 h and washed three times with the phosphate buffer. The blocks were dehydrated in a graded series of ethanol for 15 min at each step and then in absolute acetone for 20 min twice. For SEM, the blocks were dehydrated in an HCP-2 critical point dryer (Hitachi, Tokyo, Japan) and then coated with gold-palladium in an E-1010 ion sputter (Hitachi, Tokyo, Japan) and observed with an Gemini SEM 300 scanning electron microscope (Carl Zeiss, Jena, Germany). For TEM, the dehydrated blocks were infiltrated with graded series of Spurr’s resin mixed with absolute acetone and then the Spurr’s resin overnight. The blocks embedded in the Spurr’s resin were polymerized at 70°C for 12 h. The specimen was sectioned with an EM UC7 ultratome (Leica Microsystems, Vienna, Austria). The ultrathin sections were stained with uranyl acetate and lead citrate and then observed with an H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
3
Ultrastructural Analysis of FSIP2 Variant Sperm
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Fresh semen samples from normal controls and men carrying FSIP2 variants were washed three times with 1× phosphate-buffered saline (PBS), centrifuged at 2500 rpm for 10 min and then fixed with 2.5% glutaraldehyde (pH 6.9) for 2 h at 4 °C.
For SEM, fixed spermatozoa were progressively dehydrated using increasing concentrations of ethanol (30%, 50%, 70%, 80%, 90%, and 100%) and then dried in a K850 CO2 critical point dryer (Quorum Technologies, Lewes, UK). The specimens were then coated with metal particles using a Cressington 108 Auto carbon coater (Cressington Scientific Instruments Ltd, Watford, UK) and analyzed under a GeminiSEM 300 scanning electron microscope (ZEISS, Oberkochen, Germany).
For TEM, fixed spermatozoa were post-fixed with 1% osmium tetroxide for 2 h at 4 °C, dyed with 2% uranium acetate for 2 h, and dehydrated in a graded series of ethanol (50%, 70%, 90%, and 100%) and 100% acetone. The fixed spermatozoa were then embedded in EPON 812 epoxy resin. Finally, the embedded spermatozoa were sliced into ultrathin sections (100nm thick), stained with lead citrate, and observed under a Talos L120C G2 transmission electron microscope (Thermo Fisher Scientific, Waltham, MA, USA).
For SEM, fixed spermatozoa were progressively dehydrated using increasing concentrations of ethanol (30%, 50%, 70%, 80%, 90%, and 100%) and then dried in a K850 CO2 critical point dryer (Quorum Technologies, Lewes, UK). The specimens were then coated with metal particles using a Cressington 108 Auto carbon coater (Cressington Scientific Instruments Ltd, Watford, UK) and analyzed under a GeminiSEM 300 scanning electron microscope (ZEISS, Oberkochen, Germany).
For TEM, fixed spermatozoa were post-fixed with 1% osmium tetroxide for 2 h at 4 °C, dyed with 2% uranium acetate for 2 h, and dehydrated in a graded series of ethanol (50%, 70%, 90%, and 100%) and 100% acetone. The fixed spermatozoa were then embedded in EPON 812 epoxy resin. Finally, the embedded spermatozoa were sliced into ultrathin sections (100nm thick), stained with lead citrate, and observed under a Talos L120C G2 transmission electron microscope (Thermo Fisher Scientific, Waltham, MA, USA).
4
Electrochemical Detection of Aflatoxins
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Potassium ferricyanide and potassium ferrocyanide were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). We obtained rGO, chloroauric acid, triclofos sodium, and 6-mercaptoethanol (MCH) from Shanghai Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). The sequences (Table 1) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). We obtained AFM1, aflatoxin M2, aflatoxin B1, aflatoxin G1 and aflatoxin G2 (>99%) from Pribolab Pte. Ltd. (Singapore). The sample of pure milk was purchased from Yonghui Supermarket (Chongqing, China).
All electrochemical measurements were conducted on the CHI600E workstation (Shanghai CH Instruments, China). The 3-electrode cell was used, where the GCE was chosen as the working electrode, the saturated calomel electrode as the reference electrode, and the platinum wire as the counter-electrode. Scanning electron microscopy was conducted using a Gemini SEM 300 scanning electron microscope (Carl Zeiss AG, Germany). Deionized water was purified using a Millipore (Bedford, MA) Milli-Q Ultrapure Water System and used for all experiments.
All electrochemical measurements were conducted on the CHI600E workstation (Shanghai CH Instruments, China). The 3-electrode cell was used, where the GCE was chosen as the working electrode, the saturated calomel electrode as the reference electrode, and the platinum wire as the counter-electrode. Scanning electron microscopy was conducted using a Gemini SEM 300 scanning electron microscope (Carl Zeiss AG, Germany). Deionized water was purified using a Millipore (Bedford, MA) Milli-Q Ultrapure Water System and used for all experiments.
5
Comprehensive Materials Characterization Techniques
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X-ray diffraction (XRD) data was acquired from a LabX XRD-6100 X-ray diffractometer with a Cu Kα radiation (40 kV, 30 mA) of wavelength of 0.154 nm (SHIMADZU, Japan). Scanning electron microscope (SEM) images were collected on a GeminiSEM 300 scanning electron microscope (ZEISS, Germany) at an accelerating voltage of 5 kV. Transmission electron microscopy (TEM), high-angle annular dark-field scanning TEM (HAADF-STEM), and Energy dispersive spectrometer (EDS) images were acquired on a JEM-2800 electron microscope (JEOL, Japan) operated at 200 kV. X-ray photoelectron spectroscopy (XPS) measurements were performed on an ESCALABMKII X-ray photoelectron spectrometer using Mg as the exciting source.
6
Comprehensive Structural and Compositional Analysis
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Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images of the as-prepared samples were recorded on a Gemini SEM 300 scanning electron microscope (Zeiss, Oberkochen, Germany) and a JEOL 2000 EX electronic microscope (JEOL, Tokyo, Japan), respectively. High angle annular dark-field scanning TEM (HAADF-STEM) and energy dispersive X-ray spectroscopy (EDS) mapping images were collected on a FEI Talos F200X TEM electron microscope at 200 kV (Thermo Fisher Scientific, USA). The X-ray photoelectron spectroscopy (XPS) spectra were collected on an ESCALAB 250 photoelectron spectrometer (Thermo-VG Scientific, USA) using Al Ka (1486.6 eV) as the X-ray source. Powder X-ray powder diffraction (PXRD) of the sample was performed on an X-ray powder diffractometer using CuKa radiation. The surface areas of the samples were calculated from nitrogen adsorption/desorption measurements on a Quadrasorb SI4 adsorptometer (Quantachrome, Boynton Beach, USA). Fourier-transform infrared spectroscopy (FT-IR) characterization was performed on a Thermo Nicolet 380 spectrometer using KBr pellets (Nicolet, Wisconsin, USA). Hydroxyl radicals were measured using a JEOL JES-FA200 electron spin resonance (ESR) spectrometer.
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