Poly d lysine

Manufactured by R&D Systems

Poly-D-Lysine is a synthetic polymer used as a coating material for cell culture surfaces. It promotes cell adhesion and growth by enhancing the attachment of cells to the culture substrate.

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Lab products found in correlation

Trypsin neutralization solution, by ScienCell (1 mentions) Hcmec d3 cells, by Merck Group (1 mentions) Astrocyte medium, by ScienCell (1 mentions) Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Trypsin, by Merck Group (1 mentions) Dnase 1, by Merck Group (1 mentions)

3 protocols using poly d lysine

Dissection and Imaging of Polarized C. elegans Embryos

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Generally, gravid C. elegans were dissected in egg salts (5 mM HEPES pH 7.4, 118 mM NaCl, 40 mM KCl, 3.4 mM MgCl_2, 3.4 mM CaCl₂) and mounted on 2% agarose pads. For tubulin experiments, C. elegans were dissected in egg salts plus 50 μg/mL nocodazole or DMSO.
For MLN8237 inhibitor experiment: After perm-1 depletion, C. elegans were dissected in embryonic imaging medium (50% L-15 Leibovitz’s Medium [Gibco], 10% 5 mg/mL Inulin, 20% FBS [Gibco], 20% 250 mM HEPES pH 7.5) on a Poly-D-Lysine (R&D Systems)-coated coverslip mounted on a slide with double-sided tape (3M) used as spacer. Additional imaging media was flowed into fill the chamber. Fifty μM MLN8237 or DMSO was flowed into the embryonic chamber at 100 s post NEB while imaging.
For imaging early polarization: Worms were dissected into embryonic imaging medium (50% L-15 Leibovitz’s Medium [Gibco], 10% 5 mg/mL Inulin, 20% FBS, 20% 250 mM HEPES, pH 7.5) on a Poly-D-Lysine (R&D Systems)-coated coverslip mounted on a slide with double-sided tape (3M) used as spacer. Additional media was flowed into fill the chamber before imaging.

Longhini K.M, & Glotzer M. (2022). Aurora A and cortical flows promote polarization and cytokinesis by inducing asymmetric ECT-2 accumulation. eLife, 11, e83992.

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Generation and Culture of hiPSC-Derived Neurons and Astroglia

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hCMEC/D3 cells (Cat #SCC066) and media (Cat #SCME004), dish coating material type I rat collagen (Cat #08–115) were purchased from MilliporeSigma. Primary human astrocytes isolated from cerebral cortex (Cat # 1800), astrocyte medium (Cat # 1801), trypsin neutralization solution (Cat # 0113) and dish coating material poly-L-lysine (Cat # 0403) were purchased from ScienCell Research Laboratories. All cells were maintained according to manufacturer instructions. Generation and culture of hiPSC-derived neurons and astroglia from human cortical spheroids (hCS) were described previously.¹⁷ (link)^,²⁶ (link)^,³⁶ (link) Briefly, hiPSC derived from fibroblasts and maintained on MEFs were used to generate hCS for more than 150 days, after which they were enzymatically dissociated and immunopanned into purified neurons (Mouse anti-Thy1 [CD90], BD Biosciences, Cat. 550402) and astroglia cells (Mouse anti-HepaCAM, R&D, Cat # MAB4108) as previously described.²⁶ (link) The isolated cells were seeded on poly-d-lysine coated plates and maintained in a Neurobasal/DMEM based serum-free medium.²⁶ (link) All cell cultures were maintained in a humidified incubator at 37°C with 5% CO₂. All human stem cell work was performed with approval from the Stanford Human Stem Cell Research Oversight committee.

Song R., Pekrun K., Khan T.A., Zhang F., Paşca S.P, & Kay M.A. (2022). Selection of rAAV vectors that cross the human blood-brain barrier and target the central nervous system using a transwell model. Molecular Therapy. Methods & Clinical Development, 27, 73-88.

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Isolation and Culture of Mouse DG Granule Neurons

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Unless otherwise specified, all reagents used for neuron culture were from Thermo Fisher Scientific.
Dissection and culturing of DG granule cells from P6-8 mouse pups were generally following the procedures reported previously 46 (link) with some changes. Briefly, DG was dissected in Hibernate-A medium, and then digested by HBSS/0.1% Trypsin (Sigma)/0.1% DNase I (Sigma). The dissociated GC preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted November 1, 2022. ; https://doi.org/10.1101/2022.10.31.514574 doi: bioRxiv preprint 13 neurons were then suspended with the plating medium containing Neurobasal-A, 10% FBS, GlutaMAX-1 (1×) and penicillin-streptomycin (1×). For the axon growth assay, the GC neurons were pipetted into the soma compartments of the microfluidic chambers coated with poly-D-lysine (R&D). After 0.5-1 hr, when the neurons were attached, the culturing medium composed of Neurobasal-A, B27 (1×), GlutaMAX1 (1×) and penicillin-streptomycin (1×) was added to the chambers. For other experiments without using the microfluidic chambers, GC neurons were plated to the 24-well plates coated with poly-D-lysine with plating medium, which was replaced with culturing medium 4 hr later.

Zhuang M., Geng X., Han P., Che P., Liang F., Liu C., Yang L., Yu J., Zhang Z., Dong W, & Ji S.J. (2023). YTHDF2 in dentate gyrus is the m⁶A reader mediating m⁶A modification in hippocampus-dependent learning and memory. Molecular psychiatry, 28(4).

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