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Cd3 cd8 dynabeads

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CD3/CD8 Dynabeads are magnetic beads coated with antibodies specific to the CD3 and CD8 surface markers. They are designed for the isolation and enrichment of T cells from biological samples.

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Lab products found in correlation

Easysep human cd8 t cell isolation kit, by STEMCELL (2 mentions) Easysep mouse cd8 t cell isolation kit, by STEMCELL (2 mentions) Click medium, by Merck Group (2 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions) Histopaque, by Merck Group (2 mentions) Tegfr antibody, by BioLegend (2 mentions)

Spelling variants of this product

Dynabeads (2112 citations) Dynabeads CD8 (29 citations) Dynabeads CD3 (25 citations)

4 protocols using cd3 cd8 dynabeads

1

Isolation and Culture of Human and Mouse CD8+ T Cells

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Buffy coats from de-identified healthy human blood donors were purchased from the Gulf Coast Regional Blood Center (Houston, TX); their acquisition or this purpose was approved by the MD Anderson Institutional Review Board. Peripheral blood mononuclear cells were isolated from buffy coat samples via centrifugation using the Ficoll-Paque method. Human CD8+ T cells were enriched from peripheral blood mononuclear cells using an EasySep human CD8+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada). These cells were cultured in a mixture comprising 45% RPMI-1640 medium, 45% Click medium (Sigma-Aldrich, St. Louis, MO), and 10% fetal bovine serum, referred to in some experiments as regular medium. Mouse CD8+ T cells were enriched from splenocytes using an EasySep mouse CD8+ T cell isolation kit (Stemcell Technologies). Mouse T cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, and were activated with CD3/CD8 Dynabeads (Thermo Fisher Scientific, Waltham, MA), IL-12 (10 ng/mL), and IL-2 (50 U/mL) recombinant proteins. Supernatants of mouse T cell cultures after 24 h were used as conditioned medium.
Hu J., Xia X., Gorlick R, & Li S. (2019). Induction of NKG2D ligand expression on tumor cells by CD8+ T cell engagement-mediated activation of nuclear factor-kappa B and p300/CBP-associated factor. Oncogene, 38(49), 7433-7446.
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2

Isolation and Culture of Human and Mouse CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Buffy coats from de-identified healthy human blood donors were purchased from the Gulf Coast Regional Blood Center (Houston, TX); their acquisition or this purpose was approved by the MD Anderson Institutional Review Board. Peripheral blood mononuclear cells were isolated from buffy coat samples via centrifugation using the Ficoll-Paque method. Human CD8+ T cells were enriched from peripheral blood mononuclear cells using an EasySep human CD8+ T cell isolation kit (Stemcell Technologies, Vancouver, BC, Canada). These cells were cultured in a mixture comprising 45% RPMI-1640 medium, 45% Click medium (Sigma-Aldrich, St. Louis, MO), and 10% fetal bovine serum, referred to in some experiments as regular medium. Mouse CD8+ T cells were enriched from splenocytes using an EasySep mouse CD8+ T cell isolation kit (Stemcell Technologies). Mouse T cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, and were activated with CD3/CD8 Dynabeads (Thermo Fisher Scientific, Waltham, MA), IL-12 (10 ng/mL), and IL-2 (50 U/mL) recombinant proteins. Supernatants of mouse T cell cultures after 24 h were used as conditioned medium.
Hu J., Xia X., Gorlick R, & Li S. (2019). Induction of NKG2D ligand expression on tumor cells by CD8+ T cell engagement-mediated activation of nuclear factor-kappa B and p300/CBP-associated factor. Oncogene, 38(49), 7433-7446.
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3

T Cell Transduction with Truncated EGFR and murPRAME-TCR

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Leukapheresis samples from two healthy donors were purchased from the NIH Blood Bank (Bethesda, MD). Peripheral blood mononuclear cells (PBMC) were isolated using Histopaque (Sigma, Cat. 10771). T cells were stimulated using CD3/CD8 Dynabeads (Invitrogen, Cat. 11131D) in the presence of IL-2 (40IU, PeproTech, Cat. 200–02). After 48 hours of stimulation, T cells were transduced with a lentiviral vector containing truncated EGFR and murPRAME-TCR. Transduction efficiency was determined 4 days after transduction by flow cytometry using a tEGFR antibody (BioLegend, Cat. 352903).
Brohl A.S., Sindiri S., Wei J.S., Milewski D., Chou H.C., Song Y.K., Wen X., Kumar J., Reardon H.V., Mudunuri U.S., Collins J.R., Nagaraj S., Gangalapudi V., Tyagi M., Zhu Y.J., Masih K.E., Yohe M.E., Shern J.F., Qi Y., Guha U., Catchpoole D., Orentas R.J., Kuznetsov I.B., Llosa N.J., Ligon J.A., Turpin B.K., Leino D.G., Iwata S., Andrulis I.L., Wunder J.S., Toledo S.R., Meltzer P.S., Lau C., Teicher B.A., Magnan H., Ladanyi M, & Khan J. (2021). Immuno-transcriptomic profiling of extracranial pediatric solid malignancies. Cell reports, 37(8), 110047.
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4

T Cell Transduction with Truncated EGFR and murPRAME-TCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leukapheresis samples from two healthy donors were purchased from the NIH Blood Bank (Bethesda, MD). Peripheral blood mononuclear cells (PBMC) were isolated using Histopaque (Sigma, Cat. 10771). T cells were stimulated using CD3/CD8 Dynabeads (Invitrogen, Cat. 11131D) in the presence of IL-2 (40IU, PeproTech, Cat. 200–02). After 48 hours of stimulation, T cells were transduced with a lentiviral vector containing truncated EGFR and murPRAME-TCR. Transduction efficiency was determined 4 days after transduction by flow cytometry using a tEGFR antibody (BioLegend, Cat. 352903).
Brohl A.S., Sindiri S., Wei J.S., Milewski D., Chou H.C., Song Y.K., Wen X., Kumar J., Reardon H.V., Mudunuri U.S., Collins J.R., Nagaraj S., Gangalapudi V., Tyagi M., Zhu Y.J., Masih K.E., Yohe M.E., Shern J.F., Qi Y., Guha U., Catchpoole D., Orentas R.J., Kuznetsov I.B., Llosa N.J., Ligon J.A., Turpin B.K., Leino D.G., Iwata S., Andrulis I.L., Wunder J.S., Toledo S.R., Meltzer P.S., Lau C., Teicher B.A., Magnan H., Ladanyi M, & Khan J. (2021). Immuno-transcriptomic profiling of extracranial pediatric solid malignancies. Cell reports, 37(8), 110047.
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