Lck cre transgenic mice

Fbxl12 conditional knockout mice were generated with a targeting vector purchased from the Knockout Mouse Project (KOMP) repository (http:www.komp.org). The vector was linearized and transfected into B6 embryonic stem (ES) cells. Transfected ES cells were cultured with media containing neomycin and resistant clones were screened for homologous recombination by PCR. Blastocyst injections resulted in several chimeric mice, three of which gave germline transmission. Germline Fbxl12^fl/+ mice were crossed to ROSA26::FLPe mice (Jackson labs; Stock no. 003946) to delete the Neo gene. Offspring were then crossed to generate Fbxl12^fl/fl mice. Fbxl1^−/− mice^{51 (link)} were provided by Dr.Liang Zhu (Albert Einstein College of Medicine). Cdkn1b^fl/fl mice^{52 (link)} were purchased from Jackson Laboratory (Stock no. 027328). Lck-Cre transgenic mice, AND TCR-transgenic mice, Rag2^−/− mice and CD45.1 C57BL/6 mice were obtained from Taconic Biosciences. Animal experiments were approved by the Animal Care and Use Committee of the National Institute of Child Health and Human Development.

C57BL/6 (B6), Rag2^−/−, AND TCR-transgenic mice (26 (link)), were obtained from the Jackson Laboratory. AND mice were crossed with Rag2^−/−mice to prevent rearrangement of the endogenous TCRα locus. Lck-Cre-transgenic mice were obtained from Taconic. Nur77^−/− mice (27 (link)) were obtained from C. Benoist. Nr3c1 (GR) exon 3 conditionally-targeted mice were described (9 (link)). Helios conditionally-targeted (28 (link)) and EGFP reporter (29 ) mice were kindly provided by Angela Thornton. All mice used in this study were backcrossed for at least seven generations onto B6. Primer sequences used for genotyping are provided in Supplemental Table I.