P stat1

Fluvastatin capsules were purchased from Novartis Pharmaceutical Co., Ltd. (Beijing, China). A Masson staining kit was purchased from Leagene Biotechnology Co., Ltd. (Beijing, China). A hematoxylin-eosin staining kit was purchased from Servicebio Technology Co., Ltd. (Wuhan, China). Cell Counting Kit-8 (CCK-8) was obtained from Fcmacs Biotech Co., Ltd. (Nanjing, China). A BCA protein assay kit was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Rosiglitazone (RSG, selective PPAR-γ agonist) and T0070907 (selective PPAR-γ antagonist) were obtained from Selleck Chemicals (Houston, USA). LPS was purchased from Sigma Chemical (St. Louis, USA). Recombinant rat IL-4 and INF-γ were purchased from PeproTech Inc. (New Jersey, USA). ELISA kits (IL-1, IL-6, TNF-α, IL4, IL-10, IL-13, and TGF-β1) were obtained from JinYiBai Biological Technology (Nanjing, China). The antibodies (JAK2, STAT6, and p-STAT1) were purchased from Santa Cruz Biotechnology (California, USA). The antibodies (PPAR-γ, STAT1, and p-PPAR-γ) were purchased from Bioss (Beijing, China). The antibodies (SOCS1, SOCS3, p-JAK2, and p-STAT6) were purchased from Affinity Biosciences. APC-anti-mouse CD86 was purchased from BioLegend (San Diego, USA). PE-Cy7-anti-mouse CD206 and Intracellular Fixation & Permeabilization set were purchased from Thermo Fisher Scientific (Massachusetts, USA).

Each expression was detected with reference to existing work [18 (link)]. The harvested cells were extracted by means of radioimmunoprecipitation assay buffer (Sigma-Aldrich Co.) and then quantified by means of a bicinchoninic acid protein assay kit (Thermo Fisher Scientific). The total protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA, USA). The membranes were incubated with primary antibodies (PARP, caspase-3, PD-L1, phosphorylated signal transducer and activator of transcription 1 (pSTAT1), STAT1, actin, and GAPDH; Santa Cruz Biotechnology, Inc., Dallas, TX, USA). An enhanced chemiluminescence reagent (DoGenBio, Seoul, Republic of Korea) was used for signal development.

Antibodies for B7-H3, CD-63, p-STAT1, STAT1, and Actin were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). B7-H3 overexpression plasmid (B7-H3 OE) was purchased from Sino Biological Inc. (Wayne, PA, USA, HG-11188).

miR-29b/a plasmid was purchased from Addgene (Cambridge, MA, USA). Plasmids for shB7-H3 were purchased from Origene (Rockville, MD, USA). B7-H3 overexpression plasmid (B7-H3 OE) was purchased from Sino Biological Inc. (Wayne, PA, USA). A pCDNA3-scrambled vector was used as a control. Antibodies for B7-H3, c-Myc, p-STAT1, STAT1, IGF-1R, Cofilin, N-cadherin, CDK-6, Caspase 3, and Actin were purchased from Santa Cruz Biotechnology Inc (Dallas, TX, USA). shMYC siRNA was also purchased from Santa Cruz. HRP conjugated secondary antibodies were purchased from Novus Biologicals (Littleton, CO, USA). The MYC inhibitor, JQ1, was purchased from Cayman Chemicals (Ann Arbor, MI, USA). All-trans retinoic acid (ATRA) was purchased from Sigma Aldrich (St. Louis, MO, USA).