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One step turbo tmb

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One-step Turbo TMB is a ready-to-use, single-component, horseradish peroxidase (HRP) substrate solution. It is designed to provide a fast, sensitive, and consistent colorimetric detection of HRP-conjugated antibodies or other HRP-labeled biomolecules in various immunoassay applications.

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Lab products found in correlation

Eia ria plate, by Corning (2 mentions)

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TMB Turbo (5 citations) One-Step TMB (4 citations)

2 protocols using one step turbo tmb

1

NG2 Protein Binding Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Binding assays were performed on 96-well plates (EIA/RIA plate, Corning Costar) as described previously50 (link). Briefly, microtiter plates were coated with 10 μg/ml NG2 proteins in coating buffer (0.1 M NaHCO3, pH 8.3) at 4 °C overnight, and then blocked with 1% bovine serum albumin in PBS for 1 h. Plates were then incubated with the indicated proteins for 1 h in PBS. Wells were washed three times with PBS plus 0.05% tween-20 at room temperature. One-step Turbo TMB (ThermoFisher Scientific) was used as the substrate, and absorbance at 450 nm was measured with a microplate reader.
Tao L., Zhang J., Meraner P., Tovaglieri A., Wu X., Gerhard R., Zhang X., Stallcup W.B., Miao J., He X., Hurdle J.G., Breault D.T., Brass A.L, & Dong M. (2016). Frizzled are colonic epithelial receptors for Clostridium difficile toxin B. Nature, 538(7625), 350-355.
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2

NG2 Protein Binding Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Binding assays were performed on 96-well plates (EIA/RIA plate, Corning Costar) as described previously50 (link). Briefly, microtiter plates were coated with 10 μg/ml NG2 proteins in coating buffer (0.1 M NaHCO3, pH 8.3) at 4 °C overnight, and then blocked with 1% bovine serum albumin in PBS for 1 h. Plates were then incubated with the indicated proteins for 1 h in PBS. Wells were washed three times with PBS plus 0.05% tween-20 at room temperature. One-step Turbo TMB (ThermoFisher Scientific) was used as the substrate, and absorbance at 450 nm was measured with a microplate reader.
Tao L., Zhang J., Meraner P., Tovaglieri A., Wu X., Gerhard R., Zhang X., Stallcup W.B., Miao J., He X., Hurdle J.G., Breault D.T., Brass A.L, & Dong M. (2016). Frizzled are colonic epithelial receptors for Clostridium difficile toxin B. Nature, 538(7625), 350-355.
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