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Antibodies against gapdh sc 32233

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Antibodies against GAPDH (sc-32233) are primary antibodies produced to detect the GAPDH (glyceraldehyde 3-phosphate dehydrogenase) protein. GAPDH is a widely used internal control and reference protein in various molecular and cellular biology applications.

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Lab products found in correlation

Antibodies against rab10 4262, by Cell Signaling Technology (3 mentions) Pvdf membrane, by Merck Group (3 mentions) Chloroquine c6628, by Merck Group (1 mentions) P62 610832, by BD (1 mentions) Antibody against lc3b l7543, by Merck Group (1 mentions) 3 ma m9281, by Merck Group (1 mentions) Pegfp c1 plasmid, by Takara Bio (1 mentions) Alexa fluor 488 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 donkey anti goat, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 donkey anti goat, by Thermo Fisher Scientific (1 mentions) Sc 28800, by Santa Cruz Biotechnology (1 mentions) Af3220, by R&D Systems (1 mentions) Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Anti actin mab1501 antibody, by Merck Group (1 mentions) Anti dnmt1 5032, by Cell Signaling Technology (1 mentions)

6 protocols using antibodies against gapdh sc 32233

1

GST-TRAIL Apoptosis Induction Protocol

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Glutathione S-transferase (GST) TRAIL was prepared as described previously [Lin et al., 2000 (link)]. Antibodies against c-IAP-1 (AF8181), c-IAP2 (552782), FADD (556402), caspase-8 (551242), caspase-3 (559565) and p62 (610832) were from BD Biosciences (San Diego, CA, USA). Antibodies against GAPDH (sc-32233) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against XIAP (2042) was from Cell Signaling (Danvers, MA, USA). Anti-poly (ADP-ribose) polymerase (PARP, ALX-210-222) and c-FLIP (ALX-804-961-0100) were from Enzo Life Sciences (Farmingdale, NY, USA). Antibody against LC3B (L7543) was purchased from Sigma-Aldrich (St Louis, MO, USA). Wortmannin (12-338) were Calbiochem (La Jolla, CA, USA). Chloroquine (C6628) and 3MA (M9281) were from Sigma-Aldrich. The pan-caspase inhibitor z-VAD-fmk (ALX-260-039) was from Enzo Life Sciences. Caspase-Glo® 3/7 Assay Systems (G8090) and Caspase-Glo® 8 Assay Systems (G8200) were from Promega (Madison, WI 53711 USA). The FLAG-cIAP1, FLAG-cIAP2 and pEBB-XIAP (which expresses FLAG-XIAP) plasmids were from Addgene (Cambridge MA) [Hu et al., 2006 (link); Hu and Yang, 2003 (link); Yang et al., 2000 (link)]. The pEGFP-C1 plasmid was from Clontech (Mountain View, CA). The V5-c-FLIP plasmid (HsCD00445121) was purchased from DNASU Plasmid Repository.
Xu J., Xu X., Shi S., Wang Q., Saxton B., He W., Gou X., Jang J.H., Nyunoya T., Wang X., Xing C., Zhang L, & Lin Y. (2015). Autophagy-mediated degradation of IAPs and c-FLIPL potentiates apoptosis induced by combination of TRAIL and Chal-24. Journal of cellular biochemistry, 117(5), 1136-1144.
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2

Meprin Protein Quantification and Localization

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Cisplatin was purchased from Novaplus (Bedford, OH). All other chemicals were from Sigma-Aldrich unless specified otherwise. The antibodies against meprin α (AF3220 and AF4007), meprin β (AF3300), and pro-meprin β (MAB 28592) were from R&D Systems (Minneapolis, MN). The nidogen-1 antibody (MAB 1886) was from Millipore (Temecula, CA). Antibodies against GAPDH (sc-32233) and Na+/K+-ATPase (sc-28800) as well as all HRP-conjugated secondary antibodies were from Santa Cruz Biotechnology (Dallas, TX). Fluorescent-labeled secondary antibodies (Alexa Fluor 488 donkey anti-rabbit, Alexa Fluor 488 donkey anti-goat, Alexa Fluor 594 donkey anti-goat, and Alexa Fluor 594 donkey anti-rat) were from Molecular Probes (Eugene, OR).
Herzog C., Marisiddaiah R., Haun R.S, & Kaushal G.P. (2015). Basement membrane protein nidogen-1 is a target of meprin β in cisplatin nephrotoxicity. Toxicology letters, 236(2), 110-116.
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3

Quantitative Western Blot Analysis

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Western blot was performed as described previously (Wang et al. 2016) . Brie y, cells were lysed and the total proteins were extracted and quanti ed. Equivalent amounts of protein from each sample were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene uoride (PVDF) membranes (Millipore, USA). The membrane was blocked with 5% skim milk at room temperature for 1 h, followed by incubation with primary antibody (RAB10 with 1:1000 dilution, GAPDH with 1:5000 dilution) at 4°C overnight and detected by chemiluminescence. Antibodies against RAB10 (#4262) were obtained from Cell Signaling Technology (Beverly, MA, USA) and antibodies against GAPDH (sc-32233) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Peng G., Su J., Xiao S, & Liu Q. (2021). LINC00152 acts as a potential marker in gliomas and promotes tumor proliferation and invasion through the LINC00152/miR-107/RAB10 axis. Journal of neuro-oncology, 154(3).
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4

Western Blot Analysis of RAB10 and GAPDH

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Western blot was performed as described previously (Wang et al. 2016) . Brie y, cells were lysed and the total proteins were extracted and quanti ed. Equivalent amounts of protein from each sample were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene uoride (PVDF) membranes (Millipore, USA). The membrane was blocked with 5% skim milk at room temperature for 1 h, followed by incubation with primary antibody (RAB10 with 1:1000 dilution, GAPDH with 1:5000 dilution) at 4 °C overnight and detected by chemiluminescence. Antibodies against RAB10 (#4262) were obtained from Cell Signaling Technology (Beverly, MA, USA) and antibodies against GAPDH (sc-32233) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Liu Q., peng g., Su J., wang z., & xiao s. (2021). LINC00152 Acts as a Potential Marker in Gliomas and Promotes Tumor Proliferation and Invasion Through The LINC00152/miR-107/RAB10 Axis.
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5

Quantitative Western Blot Analysis

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Western blot was performed as described previously (Wang et al. 2016) . Brie y, cells were lysed and the total proteins were extracted and quanti ed. Equivalent amounts of protein from each sample were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene uoride (PVDF) membranes (Millipore, USA). The membrane was blocked with 5% skim milk at room temperature for 1 h, followed by incubation with primary antibody (RAB10 with 1:1000 dilution, GAPDH with 1:5000 dilution) at 4°C overnight and detected by chemiluminescence. Antibodies against RAB10 (#4262) were obtained from Cell Signaling Technology (Beverly, MA, USA) and antibodies against GAPDH (sc-32233) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Liu Q., peng g., Su J., & xiao s. (2021). LINC00152 Acts as a Potential Marker in Gliomas and Promotes Tumor Proliferation and Invasion Through the LINC00152/miR-107/RAB10 Axis.
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6

Antibody Validation for Protein Analysis

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All reagents were purchased from Sigma-Aldrich (Sigma-Aldrich, WI) unless otherwise indicated. Antibodies against GAPDH (sc-32233) was from Santa Cruz Biotechnology Inc., USA.
Anti-beta Actin (ab8227), -histone H2A (ab13923), histone H4 (ab10158), and HP1BP3 (ab98894) antibodies were purchased from Abcam, UK. The anti-actin antibody (MAB1501) was purchased from Millipore, USA. Anti DNMT1 (#5032) and CHD1 (#4351) were purchased from Cell Signaling Technology, MA, USA.
Dutta B., Park J.E., Qing I.T.Y., Kon O.L, & Sze S.K. (2018). Soy-Derived Phytochemical Genistein Modifies Chromatome Topology to Restrict Cancer Cell Proliferation. Proteomics, 18(16).
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