Antibodies used in immunoblotting, immunoprecipitation, and ChIP assays are listed in Supplementary Table 3 . Fluorescence-labeled antibodies are described in the section of flow cytometry and cell sorting. Human Trabid cDNA was amplified by PCR from the pHM6-HA-Trabid plasmid (provided by Dr. Mariann Bienz, Medical Research Council, UK) and inserted into the pCLXSN(GFP) retroviral vector44 (link). The catalytically inactive Trabid mutant, HA-Trabid C443A, was created by site-directed mutagenesis. Mouse Jmjd2d expression vector (pcDNA-FLAG-mKdm4d) was obtained from Addgene, and the cDNA insert was transferred to the pCLXSN(GFP) vector to generate pCLXSN(GFP)-HA-Jmjd2d. pGIPZ lentiviral vectors encoding a non-silencing shRNA control and two different Jmjd2d shRNAs were obtained from Thermo Scientific.
LPS (derived from Escherichia coli strain 0127:B8) and CpG (2216) were from Sigma-Aldrich. Poly (I:C) was from Amersham, and recombinant murine GM-CSF was from Peprotech. The Jmjd2 inhibitor 5-carboxy-8HQ was purchased from Tocris Bioscience.
LPS (derived from Escherichia coli strain 0127:B8) and CpG (2216) were from Sigma-Aldrich. Poly (I:C) was from Amersham, and recombinant murine GM-CSF was from Peprotech. The Jmjd2 inhibitor 5-carboxy-8HQ was purchased from Tocris Bioscience.