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Anti cytokeratin 18 mouse monoclonal antibody

Manufactured by Abcam

Anti-cytokeratin 18 mouse monoclonal antibody is a lab equipment product that recognizes the cytokeratin 18 protein. Cytokeratin 18 is a type I intermediate filament protein expressed in simple and stratified epithelial cells.

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2 protocols using anti cytokeratin 18 mouse monoclonal antibody

1

Antibody Immunostaining and Western Blotting Protocol

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The following antibodies were used for immunostaining and Western blotting: anti-mEcad clone ECCD-2 rat monoclonal antibody (Invitrogen), anti-hEcad clone HECD-1 mouse monoclonal antibody (Invitrogen), anti–P-Akt (Ser473) rabbit polyclonal antibody (Osenses), anti–mouse M cell clone NKM 16–2-4 rat monoclonal antibody (Miltenyi Biotec), R11 anti-Lm rabbit polyclonal antibody (Dramsi et al., 1998 (link)), anti–c-Met SP260 rabbit polyclonal antibody (Santa Cruz laboratories), anti-FoxO1 rabbit polyclonal antibody (Cell Signaling Technology), anti-cytokeratin 18 mouse monoclonal antibody (Abcam), anti-Akt mouse monoclonal antibody (Cell Signaling Technology), anti–β-actin clone AC-15 mouse monoclonal antibody (Sigma-Aldrich), phalloidin conjugated with Alexa Fluor 647 (Invitrogen), wheat germ agglutinin (WGA) conjugated with Alexa Fluor 647 (Jackson ImmunoResearch Laboratories), Alexa Fluor 488 and 568 goat anti–rabbit (Invitrogen), Alexa Fluor 546 and 647 goat anti–rat (Invitrogen), Alexa Fluor 647 goat anti–mouse (Invitrogen), and Hoechst 33342 (Invitrogen).
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2

Immunodetection of Cytokeratin 18 in Swine Cells

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Immortalized and primary swine respiratory cells were incubated for 1 h with anti-cytokeratin 18 mouse monoclonal antibody (Abcam) (1,200).
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