Immunostaining of imaginal discs was performed as previously described (Matakatsu and Blair, 2004 (link)), except that fixations were performed in PBS with 2% formaldehyde for 5 min at RT. The following primary antibodies were used: rat anti–Dachs N and C (1:20,000), guinea pig anti-App (1:20,000; Matakatsu and Blair, 2008 (link)), mouse anti–β-galactosidase (1:1,000; Developmental Studies Hybridoma Bank), rabbit anti–β-galactosidase (1:1,000; Cappel), mouse anti-V5 (1:1,000; Invitrogen), and mouse anti-CD2 (1:1,000; Serotec). Images were taken using an LSM 880 confocal microscope (ZEISS; using Zen 2.1 software) or an Apotome (ZEISS) with an ORCA-ER CCD digital camera (Hamamatsu Photonics). Plan Apochromat 40×/1.4 and Plan Apochromat 63×/1.4 oil-immersion objectives (ZEISS) were used for LSM880. The acquired images were processed with ImageJ and Photoshop (Adobe Systems). Apical z-stacks were processed to make maximal projections using ImageJ.
Mouse anti cd2
Manufactured by Bio-Rad
Sourced in United States
Mouse anti-CD2 is a laboratory reagent used for the detection and identification of the CD2 cell surface antigen. CD2 is a glycoprotein expressed on the surface of T cells and natural killer cells, and is involved in cell-cell adhesion and signaling. This product can be used in various immunoassays and flow cytometry applications to study the expression and function of CD2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 880 confocal microscope, by Zeiss (2 mentions)
Apotome, by Zeiss (2 mentions)
Mouse anti β galactosidase, by Developmental Studies Hybridoma Bank (1 mentions)
Orca er ccd digital camera, by Hamamatsu Photonics (1 mentions)
Plan apochromat 63 1.4 oil, by Zeiss (1 mentions)
Mouse anti v5, by Thermo Fisher Scientific (1 mentions)
Imagej, by Adobe (1 mentions)
Rabbit anti ph3, by Merck Group (1 mentions)
Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions)
Rat anti elav 7e8a10, by Developmental Studies Hybridoma Bank (1 mentions)
Mouse anti β galactosidase, by Merck Group (1 mentions)
Mouse anti eya, by Developmental Studies Hybridoma Bank (1 mentions)
Rabbit anti cyce, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit igg alexa594, by Thermo Fisher Scientific (1 mentions)
Anti mouse igg alexa488, by Thermo Fisher Scientific (1 mentions)
Vectashield mounting medium, by Vector Laboratories (1 mentions)
Fluoview confocal microscope, by Olympus (1 mentions)
3 protocols using mouse anti cd2
1
Immunostaining of Drosophila Imaginal Discs
2
Imaginal Disc Dissection and Immunostaining
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Imaginal discs were dissected and fixed according to standard protocols. Primary antibodies used were guinea-pig anti-Hth [74 (link)], rabbit anti-PH3 (Sigma), rabbit anti β-galactosidase (Cappel), mouse anti β-galactosidase (Sigma), mouse anti-CD2 (Serotec), rabbit anti-GFP (Molecular Probes), mouse anti-Ey (Clements et al., 2008) and rabbit anti-cyclin B [75 (link)]. Mouse anti-Eya, rat anti-ELAV (7E8A10), and mouse anti-cyclin B were from Developmental Studies Hybridoma Bank (Iowa University). Fluorescently labeled secondary antibodies were from Molecular Probes. Anti-mouse-HRP (Sigma) was used for immunoperoxidase staining. Digoxigenin labelled stg RNA probe was produced from cDNA clone LD47579 (BDGP). ImageJ was used to quantify pixel intensities (http://imagej.nih.gov/ij/ ).
3
Immunostaining of Drosophila Larval Discs
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For immunostaining, larval discs were dissected in PBS, fixed in 4% paraformaldehyde, blocked in 5% BSA, and incubated in primary antibodies overnight at 4°C, followed by secondary antibody incubation for 2 h at room temperature. The tissues were mounted with Vectashield mounting medium (Vectashield), and fluorescence images were acquired with a FluoView confocal microscope (Olympus). Primary antibodies used were rabbit anti-phospho-histone3 (Santa Cruz Biotechnology, Santa Cruz, CA, United States; 1:200), mouse anti-CD2 (AbD Serotec, 1:200), rabbit anti-CycE (Santa Cruz Biotechnology, 1:200), and goat anti-Diap1 (Santa Cruz Biotechnology, 1:200); and secondary antibodies were anti-mouse IgG Alexa 488, anti-rabbit IgG Alexa 594, and anti-goat IgG Alexa 594 (Life Technologies, 1:200).
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