Lipopolysaccharide lps from e coli 026 b6

Uric acid (HPLC quality) (Sigma-Aldrich), Lipopolysaccharide (LPS) from E. coli 026:B6 (Sigma-Aldrich), EDTA 1% (w/v) in PBS without Ca²⁺ and Mg²⁺ (Versen Biochrome, Germany), RPMI-1640 cell culture medium (Sigma-Aldrich), Tissue solubilizer “Solvable” (PerkinElmer), “Ultima Gold” scintillation cocktail (PerkinElmer), ¹⁴C labelled uric acid (50 mCi/mmol) (Hartmann Analytic GmbH). Probenecid and tranilast were purchased from Sigma-Aldrich.

α1-acid glycoprotein (AGP) C.A.T. #(66455-27-4), PBS, high pure chitosan, and lipopolysaccharide (LPS) from E. coli 026:B6 were from Sigma (L8274-10MG). The QuantiPro TM BCA Assay kit was from Sigma Aldrich; St. Louis, MO, USA (QPBCA-1KA). High purity chitosan (deacetylation degree over 75–85% mol and viscosimetric molecular weight 50–190 kDa), sodium triphosphate pentabasic (TPP), 1 M hydrochloric acid (HCl), and sodium hydroxide (NaOH) were obtained from Sigma-Aldrich (Gillingham, UK). Milli-Q water was used in all synthetic experiments. Hyaluronic acid (HA; weight average molecular weight ≈200 kDa) was purchased from Medipol SA; Lausanne, Switzerland. Regenerated cellulose (RC) dialysis membrane (MWCO 10kDa) was obtained from Spectra Por, Spectrum Laboratories Inc.; Los Angeles, CA, USA.