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Anxa1

Manufactured by R&D Systems
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ANXA1 is a protein that belongs to the annexin family. Annexins are calcium-dependent phospholipid-binding proteins involved in various cellular processes, including membrane organization, cell division, and signal transduction. ANXA1 plays a role in the regulation of inflammatory responses and the resolution of inflammation.

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Lab products found in correlation

Hspa8, by Novus Biologicals (1 mentions) Pkm2 d78a4, by Cell Signaling Technology (1 mentions) Fitc conjugated secondary antibody, by BD (1 mentions) Aldehyde sulfate latex beads, by Thermo Fisher Scientific (1 mentions) Tsg101, by Abcam (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Sb203580, by Santa Cruz Biotechnology (1 mentions) Boc 2, by MP Biomedicals (1 mentions)

4 protocols using anxa1

1

Exosome Flow Cytometry Analysis

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Exosome flow-cytometry analysis was performed as previously described15 . Briefly, exosomes preparations (15 μg) were incubated with 10 μL of 4-μm-diameter aldehyde/sulfate latex beads (Life Technologies) for 15 min at RT, then resuspended in 1 mL PBS and incubated on a test tube rotator wheel overnight at 4 °C. Free binding sites on beads were saturated with 110 μl of 1 M glycine at RT for 30 min. Exosomes-coated beads were resuspended into 400 μL PBS containing 2% BSA and a 25 μL aliquot was incubated with the following antibodies: CD63 (sc-5275, Santa Cruz), CD9 (#655433, BD Bioscience), TSG101 (ab83, Abcam), ANXA1 (AF3770, R&D Biosystems), H2AFZ (sc-67218, Santa Cruz), HSPA8 (NB100-41377, Novus), PKM2 (D78A4, Cell Signaling), and anti-human IgG (#409309, BioLegend), or an isotype-matched negative control antibody for 30 min at 4 °C followed, when needed, by incubation with FITC-conjugated secondary antibody (BD Bioscience). One microgram of primary and secondary antibody was used for all stainings. Flow-cytometry data were acquired on a Gallios flow cytometer (Beckman Coulter) and analyzed using FlowJo.
Capello M., Vykoukal J.V., Katayama H., Bantis L.E., Wang H., Kundnani D.L., Aguilar-Bonavides C., Aguilar M., Tripathi S.C., Dhillon D.S., Momin A.A., Peters H., Katz M.H., Alvarez H., Bernard V., Ferri-Borgogno S., Brand R., Adler D.G., Firpo M.A., Mulvihill S.J., Molldrem J.J., Feng Z., Taguchi A., Maitra A, & Hanash S.M. (2019). Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity. Nature Communications, 10, 254.
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2

Intracranial Hemorrhage Protocol with Annexin A1

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Recombinant human Annexin A1 protein (AnxA1, R&D Systems) and the FPR2 antagonist N-t-butyloxycarbonyl-Phe-DLeu-Phe-DLeu-Phe (Boc2; MP Biomedicals), and SB203580 (an inhibitor of p38 MAPK, Santa Cruz Biotechnology) were dissolved in 2μl sterile PBS. AnxA1 was administered i.c.v. at 0.5 hour after ICH induction. Boc2 (0.8μg/mouse) and SB203580(0.4μg/mouse) was given 1 hour before ICH induction via intracerebroventricular injection. Vehicle animals received the same volume of PBS injection. All drugs were administered to the contralateral side of hemorrhage to avoid interaction with collagenase.
Ding Y., Flores J., Klebe D., Li P., McBride D.W., Tang J, & Zhang J.H. (2019). Annexin A1 Attenuates Neuroinflammation through FPR2/p38/COX-2 Pathway after Intracerebral Hemorrhage in Male Mice. Journal of neuroscience research, 98(1), 168-178.
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3

Therapeutic effects of Annexin A1 in GMH

Cited 1 time
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Animals were divided randomly into two groups, which either received intraperitoneal or intranasal administration of Human recombinant Annexin A1 (AnxA1, R&D Systems) at a low and high concentration at 1 h after GMH induction and given for three consecutive days. Dosage concentrations for annexin A1 were modifed from the following study (Ding et al., 2020 (link)). The following groups were randomly assigned: Sham, GMH + Vehicle, GMH + AnxA1 (I⋅P, 0.5 μg/rat pup), GMH + AnxA1 (I·P 1.5 μg/rat pup), GMH + AnxA1(in 0.05 μg/ rat pup), and GMH + AnxA1 (0.0150 μg/rat pup). Hemoglobin assay and short-term neurobehavior were used to evaluate the best dosing regimen at 72 h post-ictus.
Flores J.J., Ding Y., Sherchan P., Zhang J.H, & Tang J. (2022). Annexin A1 upregulates hematoma resolution via the FPR2/p-ERK(1/2)/DUSP1/CD36 signaling pathway after germinal matrix hemorrhage. Experimental neurology, 359, 114257.
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4

Islet Incubation with ANXA1

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Human islets (100 per dish) were incubated in non-treated petri dishes containing RPMI-1640 supplemented with 10% (vol./vol.) FBS and 1% (vol./vol.) pen-strep, either alone or in the presence of ANXA1 (R&D Systems Abingdon, UK), for 2 days. Co-cultures of human islets and hASCs were performed in parallel as positive controls, as described above.
Arzouni A.A., Vargas-Seymour A., Rackham C.L., Dhadda P., Huang G.C., Choudhary P., Nardi N., King A.J.F, & Jones P.M. (2017). Mesenchymal stromal cells improve human islet function through released products and extracellular matrix. Clinical science (London, England : 1979), 131(23).
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