During recordings, slices were superfused with room temperature oxygenated aCSF at a flow rate of ~2 ml min−1. Bath aCSF contained 100 μm picrotoxin (PTX, Abcam, Cambridge, MA, USA) and 3 μm CGP 55845 hydrochloride (Abcam, Cambridge, MA, USA) to block GABAA and GABAB receptors, respectively, and to isolate excitatory synaptic currents. Where indicated, aCSF also contained one of: 10 μm forskolin, 100 μm SQ-22536 (Tocris, Bristol, UK), 1 μm JZL 184 (Tocris, Bristol, UK), 30 μm Nimesulide (Sigma-Aldrich, St Louis, MO, USA) or 1 μm WIN 55,212–2 (Tocris, Bristol, UK).
Purkinje cells were visually identified and whole-cell, patch clamp recordings were performed with borosilicate pipettes (2–4 MΩ). Access resistance was monitored throughout the experiment. Electrophysiological currents were recorded with a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA, USA),filtered at 5 kHz and digitized at 50 kHz. Data were collected using pClamp software (Molecular Devices, Sunnyvale, CA, USA). EPSCs were evoked by stimulation of parallel fibres via a patch pipette filled with aCSF.
Purkinje cells were visually identified and whole-cell, patch clamp recordings were performed with borosilicate pipettes (2–4 MΩ). Access resistance was monitored throughout the experiment. Electrophysiological currents were recorded with a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA, USA),filtered at 5 kHz and digitized at 50 kHz. Data were collected using pClamp software (Molecular Devices, Sunnyvale, CA, USA). EPSCs were evoked by stimulation of parallel fibres via a patch pipette filled with aCSF.