Nicotinamide n3376

Manufactured by Merck Group

Sourced in Sao Tome and Principe, United States

Nicotinamide (N3376) is a chemical compound that is a form of vitamin B3. It is a colorless, odorless crystalline solid that is soluble in water and alcohol. Nicotinamide is commonly used in various laboratory applications and research settings.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Rtegm bulletkit, by Lonza (1 mentions) P ampk 2535, by Cell Signaling Technology (1 mentions) Aicar, by LGC (1 mentions) Tnf α 210 ta 020, by R&D Systems (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Rabbit anti ha tag c29f4, by Cell Signaling Technology (1 mentions) Trichostatin a, by Cell Signaling Technology (1 mentions) Rabbit anti myc tag 71d10, by Cell Signaling Technology (1 mentions) Rabbit anti sirt1 c14h4, by Cell Signaling Technology (1 mentions) Anti tubulin antibody, by Merck Group (1 mentions) Anti phospho raptor ser 792, by Cell Signaling Technology (1 mentions)

3 protocols using nicotinamide n3376

AMPK Signaling in RPE Cells

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RtEGM Retinal Pigment Epithelial Cell Growth Medium (RtEGM BulletKit #195409) was purchased from Lonza (Walkersville, MD) and fetal bovine serum (#10438034) were purchased from Thermo Fisher Scientific (Waltham, MA). CFB antibody (sc-271636) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies for AMPK a1 (Abcam) : 32047,AMPKa2 (Abcam) : 3760, were from Abcam and (P-ACC (#3661), ACC (#3676), AMPK (#2603), P-AMPK (# 2535), and GAPDH (#2118) were purchased from Cell Signaling Technologies (Beverly, MA). AICAR (#A611700), a pharmacological activator of AMPK, was purchased from Toronto Research Chemicals (Toronto, ON, Canada). 5-iodotubericidin (IODO #I100), dipyridamole (DPY #D9766) and nicotinamide (N3376) were purchased from Sigma (St. Louis, MO). TNF-α (#210-TA-020) was purchased from R & D Systems (Minneapolis, MN).

Chung E.J., Efstathiou N.E., Konstantinou E.K., Maidana D.E., Miller J.W., Young L.H, & Vavvas D.G. (2017). AICAR suppresses TNF-α-induced complement factor B in RPE cells. Scientific Reports, 7, 17651.

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Antibodies and Small Molecules for Protein Analysis

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Rabbit anti-TULP1 (ab97281) and rabbit anti-TULP3 antibody (ab155317) were obtained from Abcam. HDAC Inhibitor XXIV (OSU-HDAC-44) was obtained from Calbiochem. Cycloheximide (2112S), MG-132 (2194S), rabbit antiacetylated lysine (#9441S), rabbit anti-beta actin (13E5, #4970S), rabbit anti-CUL3 (#2759S), rabbit anti-HA tag (C29F4, #3724T), mouse anti-HDAC1 (10E2, #5356S), antimouse IgG, HRP linked antibody (7076S), rabbit anti-Myc tag (71D10, #2278S), rabbit anti-P300 (D1M7C, #70088S), rabbit anti-Rad18 (D2B8, #9040S), rabbit anti-SIRT1 (C14H4, #2496S), anti-rabbit IgG, HRP linked antibody (7074S), rabbit antiubiquitin (#3933S), Trichostatin A (9950S) were obtained from Cell Signaling Technology, Inc. Rabbit anti-PP6R3 (16944-1-AP) was obtained from Proteintech. Anacardic Acid (A7236), Anti-FLAG (M2, #F1804), C646 (SML0002), EX-527 (E7034), MC1568 (M1824), mouse antiacetyl lysine (4G12, 05-515), mouse anti-TULP2 (2B5, WH0007288M3), MS-275 (EPS002), and nicotinamide (N3376) were obtained from Sigma Aldrich.

Kerek E.M., Yoon K.H., Luo S.Y., Chen J., Valencia R., Julien O., Waskiewicz A.J, & Hubbard B.P. (2020). A conserved acetylation switch enables pharmacological control of tubby-like protein stability. The Journal of Biological Chemistry, 296, 100073.

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Molecular Mechanisms of Adiponectin Regulation

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Anti-PKCδ (#2058), anti-phospho PKCδ (Thr505, #9374), anti-PPARγ (#2430), anti-AMPK (#5831), anti-phospho AMPK (Thr172, #2531; Ser485/491, #4184), anti-FoxO1 (#2880), anti-phospho FoxO1 (Ser256, #9461), anti-Raptor (#2280), and anti-phospho Raptor (Ser792, #2083) antibodies were from Cell Signaling Technology (Billerica, MA, USA). Adiponectin mouse ELISA kit (ab108785), anti-adiponectin antibody (ab181699), and anti-phospho PPARγ antibody (Ser112, ab195925) were obtained from Abcam Inc. (Cambridge, MA, USA). Wogonin (W0769), compound C (P5499), 2′,7′-dichlorofluorescein diacetate (DCHF-DA, 35845), nicotinamide (N3376), and anti-tubulin antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rottlerin (R5648), lentiviral particles carrying mouse PKCδ/ shRNA, and its control shRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Yang T., Liu H., Zhao B., Xia Z., Zhang Y., Zhang D., Li M., Cao Y., Zhang Z., Bi Y, & Wang C. (2017). Wogonin enhances intracellular adiponectin levels and suppresses adiponectin secretion in 3T3-L1 adipocytes. Endocrine journal, 64(1).

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