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Human 12 v4 beadchips

Manufactured by Illumina
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The Human-12 v4 BeadChips are a high-throughput array-based platform designed for comprehensive genetic analysis. The BeadChips provide a robust and efficient solution for simultaneously interrogating thousands of genetic markers across the human genome.

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Lab products found in correlation

Streptavidin cy3, by GE Healthcare (4 mentions) Totalprep 96 rna amplification kit, by Thermo Fisher Scientific (4 mentions) Beadarray reader, by Illumina (4 mentions) Genomestudio software, by Illumina (4 mentions) Beadchips, by Illumina (2 mentions) Beadchips, by GE Healthcare (2 mentions)

Close spelling variants of this product

BeadChips (68 citations) Human HT-12 v4.0 Expression BeadChips (22 citations) Human HT-12 v4.0 Gene Expression BeadChips (4 citations) Illumina Human HT-12 v4.0 Beadchips (4 citations)

4 protocols using human 12 v4 beadchips

1

Microarray Analysis of Transcriptome Changes

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500ng of total cellular RNA was amplified and purified using the Illumina TotalPrep-96 RNA Amplification kit (Life Technologies) according to the manufacturer’s instructions. Three biological replicates for each condition were analysed. Biotin-Labelled cRNA was then normalized to a concentration of 150ng/μl and 750ng were hybridised to Illumina Human-12 v4 BeadChips for 16 hours (overnight) at 58 °C. Following hybridisation, BeadChips were washed and stained with streptavidin-Cy3 (GE Healthcare). BeadChips were then scanned using the BeadArray reader, and image data was then processed using Genome Studio software (Illumina). The raw and processed microarray data are available on ArrayExpress (Accession number: E-MTAB-2965, https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-2965/).
Sampaziotis F., de Brito M.C., Madrigal P., Bertero A., Saeb-Parsy K., Soares F.A., Schrumpf E., Melum E., Karlsen T.H., Bradley J.A., Gelson W.T., Davies S., Baker A., Kaser A., Alexander G.J., Hannan N.R, & Vallier L. (2015). Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature biotechnology, 33(8), 845-852.
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2

Microarray Analysis of Transcriptome Changes

Check if the same lab product or an alternative is used in the 5 most similar protocols
500ng of total cellular RNA was amplified and purified using the Illumina TotalPrep-96 RNA Amplification kit (Life Technologies) according to the manufacturer’s instructions. Three biological replicates for each condition were analysed. Biotin-Labelled cRNA was then normalized to a concentration of 150ng/μl and 750ng were hybridised to Illumina Human-12 v4 BeadChips for 16 hours (overnight) at 58 °C. Following hybridisation, BeadChips were washed and stained with streptavidin-Cy3 (GE Healthcare). BeadChips were then scanned using the BeadArray reader, and image data was then processed using Genome Studio software (Illumina). The raw and processed microarray data are available on ArrayExpress (Accession number: E-MTAB-2965, https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-2965/).
Sampaziotis F., de Brito M.C., Madrigal P., Bertero A., Saeb-Parsy K., Soares F.A., Schrumpf E., Melum E., Karlsen T.H., Bradley J.A., Gelson W.T., Davies S., Baker A., Kaser A., Alexander G.J., Hannan N.R, & Vallier L. (2015). Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature biotechnology, 33(8), 845-852.
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3

Illumina Gene Expression Profiling

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500 ng of total RNA for each sample was amplified and purified using the Illumina TotalPrep-96 RNA Amplification kit (Life Technologies, UK), according to the manufacturer’s instructions. Biotin-Labelled cRNA was then normalized to a concentration of 150 ng/ul and 750 ng was hybridised to Illumina Human-12 v4 BeadChips (Illumina, CA, USA) for 16 hours (overnight) at 58°C. Following hybridisation, BeadChips were washed and stained with streptavidin-Cy3 (GE Healthcare, UK). BeadChips were then scanned using the BeadArray reader and image data was then processed using GenomeStudio software (Illumina, CA, USA).
Kilpinen H., Goncalves A., Leha A., Afzal V., Alasoo K., Ashford S., Bala S., Bensaddek D., Casale F.P., Culley O.J., Danecek P., Faulconbridge A., Harrison P.W., Kathuria A., McCarthy D., McCarthy S.A., Meleckyte R., Memari Y., Moens N., Soares F., Mann A., Streeter I., Agu C.A., Alderton A., Nelson R., Harper S., Patel M., White A., Patel S.R., Clarke L., Halai R., Kirton C.M., Kolb-Kokocinski A., Beales P., Birney E., Danovi D., Lamond A.I., Ouwehand W.H., Vallier L., Watt F.M., Durbin R., Stegle O, & Gaffney D.J. (2017). Common genetic variation drives molecular heterogeneity in human iPSCs. Nature, 546(7658), 370-375.
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4

Illumina Gene Expression Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
500 ng of total RNA for each sample was amplified and purified using the Illumina TotalPrep-96 RNA Amplification kit (Life Technologies, UK), according to the manufacturer’s instructions. Biotin-Labelled cRNA was then normalized to a concentration of 150 ng/ul and 750 ng was hybridised to Illumina Human-12 v4 BeadChips (Illumina, CA, USA) for 16 hours (overnight) at 58°C. Following hybridisation, BeadChips were washed and stained with streptavidin-Cy3 (GE Healthcare, UK). BeadChips were then scanned using the BeadArray reader and image data was then processed using GenomeStudio software (Illumina, CA, USA).
Kilpinen H., Goncalves A., Leha A., Afzal V., Alasoo K., Ashford S., Bala S., Bensaddek D., Casale F.P., Culley O.J., Danecek P., Faulconbridge A., Harrison P.W., Kathuria A., McCarthy D., McCarthy S.A., Meleckyte R., Memari Y., Moens N., Soares F., Mann A., Streeter I., Agu C.A., Alderton A., Nelson R., Harper S., Patel M., White A., Patel S.R., Clarke L., Halai R., Kirton C.M., Kolb-Kokocinski A., Beales P., Birney E., Danovi D., Lamond A.I., Ouwehand W.H., Vallier L., Watt F.M., Durbin R., Stegle O, & Gaffney D.J. (2017). Common genetic variation drives molecular heterogeneity in human iPSCs. Nature, 546(7658), 370-375.
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