Atg 12

Manufactured by Novus Biologicals

Sourced in United States

ATG-12 is a protein involved in the process of autophagy, a cellular mechanism responsible for the degradation and recycling of damaged or unnecessary cellular components. ATG-12 plays a crucial role in the formation of autophagosomes, which are key structures in the autophagy pathway. The function of ATG-12 is to form a complex with ATG5, which is essential for the initiation and progression of autophagy.

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Lab products found in correlation

β actin antibody, by Thermo Fisher Scientific (1 mentions) Igf1 r antibody, by Abcam (1 mentions) Lc3 1, by Novus Biologicals (1 mentions) Lc3 2, by Novus Biologicals (1 mentions) Procaspase 3, by Cell Signaling Technology (1 mentions) Hif 1α, by Novus Biologicals (1 mentions) Ripa buffer, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions) Phosphatase inhibitor, by Roche (1 mentions)

2 protocols using atg 12

Western Blotting of Autophagy Markers

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IGF1-R antibody was purchased from Abcam (ab182408, Cambridge, UK). β-Actin antibody was purchased from Thermo Scientific (Rockford, IL, USA). LC3-I, LC3-II, p62, ATG-7, ATG-12, BNIP3, and HIF1-α were purchased from Novus (Novus Biologicals, Centennial, CO, USA). Pro-caspase 3 was purchased from Cell Signaling Technology (Beverly, MA, USA). Pro- and cleaved caspase 9 was purchased from Abcam (Cambridge, UK).

Thomas A., Samykutty A., Gomez-Gutierrez J.G., Yin W., Egger M.E., McNally M., Chuong P., MacCuaig W.M., Albeituni S., Zeiderman M., Li M., Edil B.H., Grizzle W.E., McMasters K.M, & McNally L.R. (2020). Actively Targeted Nanodelivery of Echinomycin Induces Autophagy-Mediated Death in Chemoresistant Pancreatic Cancer In Vivo. Cancers, 12(8), 2279.

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Protein Extraction and Western Blot Analysis

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Cell lysates were harvested after treatments and timepoints indicated using RIPA buffer (Sigma, St. Louis, MO) with phosphatase inhibitors (Roche, Indianapolis, IN). Membranes were blocked in PBS-Tween 5% milk and probed with primary antibodies at manufacturer recommended concentrations. Anti-β-actin (Sigma, St. Louis, MO) was used as the protein loading control. Primary antibodies used were: ATG5, ATG12 and LC3 (Novus Biologicals, Littleton, CO).

Levy J.M., Thompson J.C., Griesinger A.M., Amani V., Donson A.M., Birks D.K., Morgan M.J., Mirsky D.M., Handler M.H., Foreman N.K, & Thorburn A. (2014). Autophagy Inhibition Improves Chemosensitivity in BRAFV600E Brain Tumors. Cancer discovery, 4(7), 773-780.

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