The LAB strains were isolated from homemade pickled beetroots. Pickled foods are widely regarded in Poland as having health-promoting properties and a being a good source of natural probiotics. A series of 10-fold dilutions ranging from 10−1 to 10−4 in Peptone Water (Peptone Water, Sigma-Aldrich, Germany) was prepared from the tested samples. The samples and all dilutions were then applied to 7 different culture media. A total of 100 μL of the appropriate dilution was applied to the medium and evenly distributed with a spreader. The prepared plates were incubated for 24–72 h in three conditions: aerobic conditions at 37 °C, aerobic conditions at 25 °C, and anaerobic conditions at 30 °C. Then, based on morphological differences, single colonies of bacteria were selected, from which reduction cultures were prepared on the same media to obtain pure cultures. The following culture media were used: M-17 Agar (Sigma Aldrich, Steinheim, Germany), MRS Agar (Sigma Aldrich, Steinheim, Germany), China Blue Lactose Agar (Sigma Aldrich, Steinheim, Germany), APT Agar (Merc, Darmstadt, Germany), and TOS (Merck, Darmstadt, Germany).
China blue lactose agar
Manufactured by Merck Group
Sourced in China
China Blue Lactose Agar is a microbiological growth medium used for the detection and enumeration of lactose-fermenting bacteria. It contains lactose as the carbohydrate source and china blue as the pH indicator dye. Lactose-fermenting bacteria will produce acid, causing the medium to turn blue, while non-lactose-fermenting bacteria will not cause a color change.
Automatically generated - may contain errors
2 protocols using china blue lactose agar
1
Isolation and Characterization of Probiotic Bacteria from Pickled Beetroots
2
Microbiological Analysis of Dairy Samples
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Dairy samples -milk, whey, buttermilk, cream, and dairy wastewater (raw effluent P1, raw effluent averager, raw effluent -centrifuge shot and treated effluent after flotation) were analyzed. Before microbiological cultures were performed, appropriate dilutions of 10 -1 , 10 -2 , 10 -3 were made for samples of milk, whey, buttermilk, cream, and for dairy wastewater 10 -3 and 10 -4 , respectively. In the next step, surface cultures were performed on 5 selected culture media: All Purpose Tween Agar (APT Agar), M-17 Agar, de MAN, ROGOSA and SHARPE Agar (MRSA) with 1% Tween80 (Sigma Aldrich, Germany), China Blue Lactose Agar (CBL), Milk Plate Count Agar (MPCA). Therefore, 100 µl of each sample was taken for this purpose and then inoculated on Petri dishes (Alchem, Poland). Most of the culture media came from Sigma Aldrich (Germany), and only the MPCA from Graso Biotech (Poland). The media were incubated for 24 h under aerobic conditions at 37°C (APT Agar, M-17 Agar, China Blue Lactose, MPCA) and under anaerobic conditions at 37°C (MRSA). To obtain pure bacterial cultures, a serial dilution method, solid medium culture and, as a last resort, reduction culture was performed.
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