Dionex ics 6000 ion chromatograph

Total solubilized orthophosphate and potassium in defined media were measured as validation of the nutrient solubilization capabilities of S. plymuthica MBSA-MJ1. Cultures were grown and inoculated in NBRIP and AKV media to evaluate phosphate and potassium solubilization, respectively. Samples were prepared similar to the solubilization assays, and after incubation and centrifugation, the supernatant was filtered through a 0.45μm nylon filter and total orthophosphate (PO₄) or potassium in the filtrate was determined using Dionex ICS-6000 Ion Chromatograph (Thermo Fisher Scientific Inc., Waltham, MA).

After the substrate sample for sequencing was collected, a 200 ml substrate (aka media) sample was collected into plastic bags and stored for nutrient analyses. Samples were prepared using the saturated media extraction (SME) protocol [38 (link)]. Saturated media/ substrate extracts were filtered using a 0.45 µm nylon filter. Filtered samples were analyzed for pH, electrical conductivity (EC), total organic carbon (TOC), total dissolved nitrogen (TDN), and nutrient ions (NO, PO₄, K, Ca, Mg, Cl, F, and SO₄). Nutrient ions were measured using a Dionex ICS-6000 Ion Chromatograph (Thermo Fisher Scientific Inc., Waltham, MA, USA). TOC and TDN were measured using Shimadzu TOC-VCPH total organic carbon analyzer with TNM-1 TN measuring unit (Shimadzu Scientific Instruments, Columbia, MD, USA) [39 (link)].