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Rabbit anti human cd31

Manufactured by Santa Cruz Biotechnology
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Rabbit anti-human CD31 is a primary antibody that recognizes the human CD31 (also known as PECAM-1) protein. CD31 is a cell adhesion molecule expressed on the surface of endothelial cells, platelets, and some immune cells. This antibody can be used for the detection and study of CD31 in various applications, such as immunohistochemistry and flow cytometry.

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Lab products found in correlation

Mouse anti α smooth muscle actin, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Fluoview fv300, by Olympus (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Alexa 594 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa 488 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Normal goat serum (ngs), by Jackson ImmunoResearch (1 mentions) Anti pecam 1, by Santa Cruz Biotechnology (1 mentions) 8 well chamber slide, by Ibidi (1 mentions) Airyscan confocal microscope, by Zeiss (1 mentions) Lsm 800, by Zeiss (1 mentions) Alexa 594 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Histoclear, by National Diagnostics (1 mentions) Alexa 488 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Sheep anti human matriptase, by R&D Systems (1 mentions) Dp70 digital camera system, by Olympus (1 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Bx40 microscope, by Olympus (1 mentions)

Close spelling variants of this product

Anti-human CD31 Rabbit anti-CD31 Anti-CD31 Rabbit anti-TM

5 protocols using rabbit anti human cd31

1

Immunofluorescence Staining of Smooth Muscle and Endothelial Cells

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The sections were deparaffinized and rehydrated. Endogenous peroxidase was inactivated with 10% hydrogen peroxide for 10 minutes. The antigens were retrieved by pepsin for 10 minutes at 37°C. The sections were blocked for 30 minutes in 10% normal goat serum (Jackson ImmunoResearch Inc., West Grove, PA, USA), followed by incubation with mouse anti-α smooth muscle actin (1 : 500; Sigma-Aldrich) and rabbit anti-human CD31 (1 : 50; Santa Cruz Biotechnology) for 1 hour at room temperature. After washing, the slides were treated with secondary antibodies including Alexa 488-conjugated goat anti-mouse IgG (1 : 1000; Invitrogen) and Alexa 594-conjugated goat anti-rabbit IgG (1 : 1000; Invitrogen) for 1 hour at room temperature. Nuclei were stained with DAPI (Sigma-Aldrich). The slides were observed using a confocal laser scanning microscope (Fluoview FV 300, Olympus, Japan).
Nam H., Kim G.H., Bae Y.K., Jeong D.E., Joo K.M., Lee K, & Lee S.H. (2017). Angiogenic Capacity of Dental Pulp Stem Cell Regulated by SDF-1α-CXCR4 Axis. Stem Cells International, 2017, 8085462.
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2

Quantifying HUVEC Surface Protein Expression

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Human umbilical vein endothelial cells (HUVECs) were grown in 8-well chamber slides (Ibidi, USA) for 3 days prior to incubat with 20 μg/ml of anti-NS1 mAbs or irrelevant anti-E mAb (4G2) for 1 h on ice. Rabbit anti-human CD31 or anti-PECAM-1 (1:10 dilution, Santa Cruz Biotechnology, USA) was used as positive control of endothelial cell surface staining11 (link),13 (link). After washing three times with DMEM containing 2% FBS, cells were incubated for 30 min on ice with goat anti-mouse IgG or anti-rabbit IgG conjugated with Alexa Fluor 488 (1:500 dilution, Thermo Fisher Scientific) and Hoechst 33,342 (1:1000 dilution, Thermo Fisher Scientific) for staining of nuclei. Stained cells were visualized and imaged with a Carl Zeiss LSM800 with Airyscan confocal microscope. The fluorescence intensity of staining (Alexa Fluor 488) on the cell surface for each clone was determined using Zeiss microscopy Zen imaging software. The fluorescence intensity of the specific protein signal was normalized by nuclear staining intensity from the entire area of each captured image.
Luangaram P., Tamdet C., Saengwong C., Prommool T., Kraivong R., Nilchan N., Punyadee N., Avirutnan P., Srisawat C., Malasit P., Kasinrerk W, & Puttikhunt C. (2022). Differential critical residues on the overlapped region of the non-structural protein-1 recognized by flavivirus and dengue virus cross-reactive monoclonal antibodies. Scientific Reports, 12, 21548.
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3

Immunofluorescent Staining of Tissue Sections

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For immunofluorescent staining, 5-μm-thick sections were deparaffinized in histoclear (National Diagnostics, Somerville, USA) and rehydrated through a series of graded alcohols and distilled water. Endogenous peroxidase activity was quenched with 10% hydrogen peroxide for 10 min, and antigen retrieval was carried out by pepsin for 10 min at 37°C. The sections were blocked for 30 min in 10% normal goat serum and incubated with primary antibodies for 1 h at room temperature. The following primary antibodies were used: rabbit anti-human CD31 (1:50; Santa Cruz Biotechnology) and mouse anti-α-smooth muscle actin (1:500; Sigma-Aldrich). Secondary antibody incubations were carried out for 1 h at room temperature using Alexa 488-conjugated goat-anti rabbit IgG (1:700; Invitrogen) and Alexa 594-conjugated goat-anti mouse IgG (1:700; Invitrogen) antibodies. All the fluorescent-stained sections were counterstained with DAPI (Sigma-Aldrich). Slides were observed using a confocal laser scanning microscope (Fluoview FV 300, Olympus, Japan).
Kim J.H., Kim G.H., Kim J.W., Pyeon H.J., Lee J.C., Lee G, & Nam H. (2016). In Vivo Angiogenic Capacity of Stem Cells from Human Exfoliated Deciduous Teeth with Human Umbilical Vein Endothelial Cells. Molecules and Cells, 39(11), 790-796.
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4

Immunohistochemical Staining of Paraffin Sections

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Antigens from 5 µm paraffin sections were retrieved by incubation for 10 min at 100°C with 1 mM EDTA, pH 8.0 for CD31 staining, or by incubation for 20 min at 100°C in 0.01 M sodium citrate buffer, pH 6.0, for all other antigens. The sections were blocked with 2% bovine serum albumin (fraction V, MP Biomedicals, Solon, OH) in phosphate-buffered saline (PBS), and incubated overnight at 4°C with 2 ug/ml rabbit anti-human CD31 (Santa Cruz Biotechnology, Santa Cruz, CA), or sheep anti-human matriptase (R&D Systems, Minneapolis, MN), primary antibodies. Bound antibodies were visualized using biotin-conjugated anti-rabbit, or -sheep secondary antibodies (Vector Laboratories, Burlingame, CA) and a Vectastain ABC kit (Vector Laboratories) using 3,3′-diaminobenzidine as the substrate (Sigma-Aldrich, St. Louis, MO). All microscopic images were acquired on an Olympus BX40 microscope using an Olympus DP70 digital camera system (Olympus, Melville, NY).
Szabo R., Peters D.E., Kosa P., Camerer E, & Bugge T.H. (2014). Regulation of Feto-Maternal Barrier by Matriptase- and PAR-2-Mediated Signaling Is Required for Placental Morphogenesis and Mouse Embryonic Survival. PLoS Genetics, 10(7), e1004470.
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5

Quantifying Vascularization in Tissue Scaffolds

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The formalin fixed samples were paraffin embedded and sectioned (5 μm thickness). The tissue orientation resulted in a radial cross section of the scaffold allowing for clear identification of the biomaterial-tissue interface. Sections were stained for hematoxylin and eosin (H&E) and Masson’s trichrome for examination of tissue structure and inflammation. Immunostains for CD31 were performed to identify blood vessels as described previously.[38 (link)] Briefly, slides were deparaffinized and rehydrated by a washing with a series of xylene and ethanol washes. Rehydrated slides underwent antigen retrieval using Dako target retrieval solution. Slides were incubated with rabbit anti-human CD31 (Santa Cruz Biotechnology, Santa Cruz, CA) and kept overnight, followed by staining with anti-rabbit secondary antibody using Vectastain Elite ABC Kit (Vector Labs, Burlingame, CA). Sections were imaged using an Axiovert 200 inverted microscope (20x objective, 0.27 μm/pixel). Vessels stained positive for CD31 were manually counted using Axiovision AC (Carl Zeiss, Germany). Blood vessel density was calculated using the following formula: vessel density = (number of CD31 stained vessels)/(tissue area).
Wang M.O., Vorwald C.E., Dreher M.L., Mott E.J., Cheng M.H., Cinar A., Mehdizadeh H., Somo S., Dean D., Brey E.M, & Fisher J.P. (2014). Evaluating 3D Printed Biomaterials as Scaffolds for Vascularized Bone Tissue Engineering. Advanced materials (Deerfield Beach, Fla.), 27(1), 138-144.
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