Mouse ifn β elisa kit

To measure IFN‐β levels, the supernatant from JIB‐04‐treated tumour cells underwent a two‐step centrifugation process. At first, the sample was subjected to centrifugation to obtain cell‐free supernatant. The mouse IFN‐β ELISA kit (PBL Assay Science, cat#42410) was used to quantify the concentration of IFN‐β, following the instruction manual. In order to identify the levels of IFN‐γ, TDLNs from mice with tumours were gathered and dissociated into single cells. Subsequently, tumour cell lysates were prepared by disrupting frozen tumour cells by three cycles of freeze–thaw cycles. These lysates were then utilised to activate tumour‐specific T cells. Following a 48‐h co‐incubation of the tumour lysates with lymph node cells, the supernatant was collected for analysis of IFN‐γ concentration using the mouse IFN‐γ ELISA kit (Proteintech, cat#KE10094).

8- and 14-week pMMR and idMMR neuroblastoma cells were cultured for 48 h, and the supernatant was removed when confluency reached ∼90%. The supernatant was centrifuged at 14,000 RPM to remove cellular debris, and IFN-β was quantified using the mouse IFN-β ELISA Kit (PBL Assay Science) according to the manufacturer’s instructions.