On target non targeting pool

To silence caveolin-1 expression, 50 nm siRNA against caveolin-1 (s2448, Life Technology) and non-targeting siRNA (ON-TARGET Non-Targeting Pool, Thermo Scientific) was transfected into VSMCs using (Thermo Scientific). Transfection was performed 48 h prior to mechanical stretch exposure.

RNA silencing was performed as described previously58 (link). Briefly, 20 nmol l⁻¹ siRNA against PARP14 (L-023583 for human cells and L-160447 for mouse cells) and PARP9 (L-014734 for human cells and L-05024901 for mouse cells; all ONTARGETplus SMART-pool, Thermo Scientific) or non-targeting siRNA (scramble control siRNA, ON-TARGET Non-Targeting Pool, Thermo Scientific) was transferred into macrophages using SilenceMag (BOCA Scientific, Boca Raton, FL), according to the manufacturer's instruction. Target sequences of siRNA pool were follows:
Human PARP14: 5′-UAAUCAAAGGUCUCUUAUG, UAAUGCUUAAGGUCCUCAU, UCAUUAUACUGCCAUUCUA-3′ and 5′-GAACUCUUGACAUCAUUUC-3′,
Human PARP9: 5′-AAUUACAUCUGCCGUCUGC-3′, 5′-UUUGUGGCAAGAAAUUCCG-3′, 5′-UUAAUCAACAGGGCUGCCA-3′ and 5′-UACAGCCAAACUUAUUCUG-3′
Mouse PARP14: 5′-CUUGAAAGCUUUACGUAUA-3′, 5′-CAGCAAUAGGAACGGGAAA-3′, 5′-CCAAAGAACUUGAUCAACA-3′ and 5′-CGUAGUAGCAAAAGCGAUA-3′,
Mouse PARP9: 5′-ACACAAUGUCUUCGAAAUU-3′, 5′-CCAGACAGCUAUCGAAUUA-3′, 5′-CCAAAUAUGAUCUACGCAU-3′ and 5′-CGUACACAUUUCAACGAUA-3′.
Control scramble: 5′-UGGUUUACAUGUCGACUAA-3′, 5′-UGGUUUACAUGUUGUGUGA-3′, 5′-UGGUUUACAUGUUUUCUGA-3′ and 5′-UGGUUUACAUGUUUUCCUA-3′.