Cell apoptosis was determined by Annexin-V-fluorescein-5-isothiocyanate (Annexin-V-FITC) apoptosis detection kit (BD PharMingen, San Diego, CA, USA) according to the manufacturer’s instructions. The cells were harvested, washed with cold PBS, and incubated with 5 µl annexin and 5 µl propidium iodide (PI, 10 mg/L) in the dark for 15 min. Then the cells were read by a FCM (Becton Dickinson, San Jose, CA, USA), and the results were analyzed using CELLQuest 3.0 software (BD Biosciences, San Jose, CA, USA).
Annexin 5 fluorescein 5 isothiocyanate annexin 5 fitc apoptosis detection kit
Manufactured by BD
Sourced in United States
Annexin-V-fluorescein-5-isothiocyanate (Annexin-V-FITC) apoptosis detection kit is a laboratory reagent used to detect and measure apoptosis, a form of programmed cell death. The kit contains Annexin-V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of apoptotic cells. Annexin-V is conjugated to the fluorescent dye FITC, allowing for the visualization and quantification of apoptotic cells using flow cytometry or fluorescence microscopy.
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2 protocols using annexin 5 fluorescein 5 isothiocyanate annexin 5 fitc apoptosis detection kit
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Annexin-V-FITC Apoptosis Assay
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Annexin V-FITC Apoptosis Assay
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To assess the level of apoptosis, Annexin V‐fluorescein‐5‐isothiocyanate (Annexin V‐FITC) apoptosis detection kit (BD Pharmingen, Beijing, USA) was used to analyse phosphatidylserine exposure according to the manufacturer's protocol. To make it short, TCMK cells in different groups were collected and washed two times with cold phosphate‐buffered saline (PBS). Then, the washed cells were centrifuged and resuspended in 500 µl 1× binding buffer, and incubated with 5 µl Annexin and 5 µl propidium iodide (PI) for about 15 min in the dark. Finally, the cells were analysed by FCM (Becton Dickinson, San Jose, CA, USA), and then, CELL Quest 3.0 software (Becton Dickinson) was used for data analysis.
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