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Treg conditioned media

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Treg-conditioned media is a cell culture medium that has been conditioned by regulatory T cells (Tregs). Tregs are a specialized subset of T cells that play a key role in immune regulation and tolerance. The media contains various factors and signaling molecules secreted by Tregs during in vitro culture.

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Lab products found in correlation

L glutamine, by Miltenyi Biotec (2 mentions) Pdgfαα, by Thermo Fisher Scientific (2 mentions) Neural medium, by Miltenyi Biotec (2 mentions) Poly l lysine coated, by BD (2 mentions) B27 macs neuro brew21, by Miltenyi Biotec (2 mentions) Pdgfαα, by R&D Systems (2 mentions) Rccn3, by R&D Systems (2 mentions) Anti ccn3 clone 231216, by R&D Systems (2 mentions) Isotype control clone 54447, by R&D Systems (2 mentions) Neural tissue dissociation kit p, by Miltenyi Biotec (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Miltenyi Biotec (2 mentions)

2 protocols using treg conditioned media

1

Generating Mixed Glial Cells from Mouse Brains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mixed glial cells were generated from male and female P2-7 C57BL/6 pups according to the protocol of the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec). In short, brains were dissected, cerebellum and meninges were removed and tissue was dissociated via mechanical and papain enzyme digestion prior to filtration through a 40 μm strainer. Cells were plated in poly-L-lysine-coated (10 μg/ml, Sigma) 96 well flat, glass-bottomed plates (BD Falcon) at a density of 105 cells per well and cultures were maintained at 37°C, 5% CO2. Cells were cultured for 5 days in DMEM (Life Technologies) supplemented with PDGFαα (10 ng/ml; PeproTech), 10% endotoxin-free FCS, 1% penicillin/streptomycin and 1% L-glutamine (Life Technologies). Cells were cultured for a further 2 days in neural medium (Miltenyi Biotec) supplemented with 1% penicillin/streptomycin, 1% L-glutamine, 2% B27/MACS Neuro Brew21 (Miltenyi Biotec) and PDGFαα (10 ng/ml; PeproTech). At day 7 in culture, PDGFαα was withdrawn to allow oligodendrocyte differentiation and cells were stimulated with 5% Treg-conditioned media, rCCN3, anti-CCN3 (clone 231216) or isotype control (clone 54447) (all R&D Systems) or controls for up to 5 days.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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2

Generating Mixed Glial Cells from Mouse Brains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mixed glial cells were generated from male and female P2-7 C57BL/6 pups according to the protocol of the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec). In short, brains were dissected, cerebellum and meninges were removed and tissue was dissociated via mechanical and papain enzyme digestion prior to filtration through a 40 μm strainer. Cells were plated in poly-L-lysine-coated (10 μg/ml, Sigma) 96 well flat, glass-bottomed plates (BD Falcon) at a density of 105 cells per well and cultures were maintained at 37°C, 5% CO2. Cells were cultured for 5 days in DMEM (Life Technologies) supplemented with PDGFαα (10 ng/ml; PeproTech), 10% endotoxin-free FCS, 1% penicillin/streptomycin and 1% L-glutamine (Life Technologies). Cells were cultured for a further 2 days in neural medium (Miltenyi Biotec) supplemented with 1% penicillin/streptomycin, 1% L-glutamine, 2% B27/MACS Neuro Brew21 (Miltenyi Biotec) and PDGFαα (10 ng/ml; PeproTech). At day 7 in culture, PDGFαα was withdrawn to allow oligodendrocyte differentiation and cells were stimulated with 5% Treg-conditioned media, rCCN3, anti-CCN3 (clone 231216) or isotype control (clone 54447) (all R&D Systems) or controls for up to 5 days.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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