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Apc conjugated cd11c

Manufactured by Thermo Fisher Scientific
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APC-conjugated CD11c is a fluorescent-labeled antibody that binds to the CD11c cell surface antigen, which is expressed on dendritic cells and some macrophages. This product is intended for use in flow cytometry applications to identify and characterize cell populations expressing CD11c.

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Lab products found in correlation

Pe conjugated cd86, by Thermo Fisher Scientific (2 mentions) Fitc conjugated cd11b, by Thermo Fisher Scientific (2 mentions) Fitc conjugated mhc 2, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

CD11c-APC (43 citations) APC-conjugated anti-CD11c (8 citations) APC conjugated anti-mouse CD11c (3 citations) APC-conjugated anti-CD11c (N418, (3 citations) APC-conjugated anti-mouse CD11c/CD25 antibodies (2 citations) APC-conjugated anti-CD11c (17-0114-82) (2 citations) APC/Cy7-conjugated anti-CD11c antibodies (1 citations)

2 protocols using apc conjugated cd11c

1

Tracking Dendritic Cell Maturation and Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols
APC-conjugated CD11c (eBioscience 17–0114), FITC-conjugated CD11b (eBioscience 11–0112) stains were conducted for DC and leukocyte recruitment analysis, and APC-conjugated CD11c, FITC-conjugated MHC II (eBioscience 11–5332), and PE-conjugated CD86 (eBioscience 12–0862) stains were conducted for DC maturation analysis. Cells were gated according to positive FITC, APC and PE using isotype controls, and the percentages of cells staining positive for each surface antigen were recorded. To track DC emigration to lymph nodes (LNs), OVA labeled with Alexa Fluor 647 was loaded in MSRs and injected subcutaneously. The inguinal lymph nodes were harvested at 7 days post injection. Cell suspensions from LNs were prepared by mechanical disruption and pressing of the tissue through 40 µm cell strainers, and examined for CD11c+ AF647+ cell numbers by flow cytometry. All flow cytometry antibodies were diluted according to the manufacturer's suggestions.
Kim J., Li W.A., Choi Y., Lewin S.A., Verbeke C.S., Dranoff G, & Mooney D.J. (2014). Injectable, spontaneously assembling inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy. Nature biotechnology, 33(1), 64-72.
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2

Tracking Dendritic Cell Maturation and Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols
APC-conjugated CD11c (eBioscience 17–0114), FITC-conjugated CD11b (eBioscience 11–0112) stains were conducted for DC and leukocyte recruitment analysis, and APC-conjugated CD11c, FITC-conjugated MHC II (eBioscience 11–5332), and PE-conjugated CD86 (eBioscience 12–0862) stains were conducted for DC maturation analysis. Cells were gated according to positive FITC, APC and PE using isotype controls, and the percentages of cells staining positive for each surface antigen were recorded. To track DC emigration to lymph nodes (LNs), OVA labeled with Alexa Fluor 647 was loaded in MSRs and injected subcutaneously. The inguinal lymph nodes were harvested at 7 days post injection. Cell suspensions from LNs were prepared by mechanical disruption and pressing of the tissue through 40 µm cell strainers, and examined for CD11c+ AF647+ cell numbers by flow cytometry. All flow cytometry antibodies were diluted according to the manufacturer's suggestions.
Kim J., Li W.A., Choi Y., Lewin S.A., Verbeke C.S., Dranoff G, & Mooney D.J. (2014). Injectable, spontaneously assembling inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy. Nature biotechnology, 33(1), 64-72.
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