Qubit rna br assay kit

Manufactured by Qiagen

The Qubit RNA BR Assay Kit is a laboratory equipment product designed for the quantification of RNA samples. It provides a sensitive and accurate method for measuring RNA concentration in a wide range of sample types. The kit utilizes fluorescent dyes that specifically bind to RNA, allowing for the determination of RNA concentration through a fluorometric-based assay.

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Lab products found in correlation

Qubit 2.0 fluorimeter, by Qiagen (2 mentions) Qubit dna dsdna br assay kit, by Qiagen (2 mentions) Qiamp dna ffpe tissue kit, by Qiagen (1 mentions) Recoverall total nucleic acid isolation kit for ffpe, by Thermo Fisher Scientific (1 mentions) Qiaamp dna kit, by Qiagen (1 mentions) Magcore total rna ffpe one step kit, by RBC Bioscience (1 mentions) Magcore automated nucleic acid extractor, by RBC Bioscience (1 mentions)

Spelling variants of this product

RNA BR kit (3 citations)

2 protocols using qubit rna br assay kit

DNA and RNA Extraction from FFPE Tissues

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DNA was extracted from melanoma, CRC and GIST samples using QIAmp DNA FFPE Tissue Kit (Qiagen) following manufacturer’s protocol (excluding de-paraffinisation). Dual DNA and RNA isolation was performed from NSCLC tissues using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Thermo Fisher Scientific). DNA and RNA concentrations were determined by fluorometric quantitation using Qubit 2.0 Fluorimeter with Qubit DNA dsDNA BR Assay Kit and Qubit RNA BR Assay Kit (Qiagen) as appropriate.

Williams H.L., Walsh K., Diamond A., Oniscu A, & Deans Z.C. (2018). Validation of the Oncomine™ focus panel for next-generation sequencing of clinical tumour samples. Virchows Archiv, 473(4), 489-503.

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Microdissection and Nucleic Acid Extraction

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Invasive tumor areas, identified on serial hematoxylin/eosin (H&E) sections by a pathologist, were harvested from 3 or 4 whole 5-μm paraffin sections by microdissection. DNA was isolated by using the QIAamp DNA kit (Qiagen, Milan, Italy) and RNA by using the MagCore Total RNA FFPE One-Step Kit with the MagCore Automated Nucleic Acid Extractor (RBC Bioscience, Diatech Labline, Jesi, Italy), according to the manufacturers’ instructions. DNA and RNA concentrations were determined by fluorometric quantitation by using a Qubit 2.0 Fluorimeter with Qubit DNA dsDNA BR Assay Kit and Qubit RNA BR Assay Kit (Qiagen) as appropriate.

Terrenato I., Ercolani C., Di Benedetto A., Gallo E., Melucci E., Casini B., Rollo F., Palange A., Visca P., Pescarmona E., Melis E., Gallina F., Sacconi A., Cecere F.L., Landi L., Cappuzzo F., Ciliberto G, & Buglioni S. (2022). A Real-World Systematic Analysis of Driver Mutations’ Prevalence in Early- and Advanced-Stage NSCLC: Implications for Targeted Therapies in the Adjuvant Setting. Cancers, 14(12), 2971.

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