MgCl2, (NH4)2SO4, 2-mercaptoethanol, Tris–HCl, ZnCl2, glycerol, l -ascorbic acid, citric acid, NaBH4, and N2H4·H2O were purchased from Sigma-Aldrich (St. Louis, MO). Sodium citrate and sodium phosphate monohydrate were purchased from EM Science (Billerica, MA). H2PtCl6·6H2O (99.9%) was purchased from Strem Chemicals (Newburyport, MA). Tween 20 and NaCl were obtained from Fisher Scientific (Pittsburgh, PA). All reagents were used as received. The DNA conjugates, ssDNA (5′–(CH2)3–SH CAC GAC GTT GTA AAA CGA CGG CCA G-3′) and Cy3–ssDNA (5′–(CH2)3–SH CAC GAC GTT GTA AAA CGA CGG CCA G-Cy3-3′), were from Integrated DNA Technologies (Coralville, IA) and received as lyophilized pellets in microcentrifuge tubes. The pellets were centrifuged to ensure no residue on the walls or cap remained, and then suspended in H2O. Deionized (DI) water having a resistivity of 18.2 MΩ cm was used for all experiments (Milli-Q gradient water purification system, Millipore, Bedford, MA). Experiments were conducted at room temperature (23 ± 2 °C). Unless otherwise stated, the phosphate buffer was adjusted to pH 7.
N2h4 h2o
Manufactured by Merck Group
N2H4·H2O is a chemical compound that serves as a laboratory reagent. It is the monohydrate form of hydrazine.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Tris hcl, by Merck Group (1 mentions)
Milli q gradient water purification system, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Zncl2, by Merck Group (1 mentions)
Nh4 2so4, by Merck Group (1 mentions)
Tween 20, by Thermo Fisher Scientific (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Nabh4, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Nicl2 6h2o, by Junsei (1 mentions)
Tetrachloroauric acid haucl4, by Merck Group (1 mentions)
Hydrazine hydrate solution, by Merck Group (1 mentions)
1 4 dinitrobenzene dnb, by Merck Group (1 mentions)
Haucl4, by Merck Group (1 mentions)
Na2teo3, by Merck Group (1 mentions)
Rf 6000 spectrofluorophotometer, by Shimadzu (1 mentions)
Professional meter pp 50, by Sartorius (1 mentions)
8 protocols using n2h4 h2o
1
DNA-Conjugate Synthesis and Characterization
2
Ni-Doped Graphite Catalyst Synthesis
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For the Ni adsorption on the graphite, 50 g of pristine graphite (MCMB, Osaka gas), 6 g of nickel chloride hexahydrate (NiCl2· 6H2O, >97.0%, JUNSEI), and 0.2 g of sodium hydroxide (anhydrous NaOH, >98.0%, bead form, SAMCHUN) were dissolved in methanol (>99.5%, SAMCHUN)/deionized water (5:5, v/v), followed by the addition of 1 ml of hydrazine monohydrate (N2H4·H2O, 98.0%, Sigma-Aldrich). The solution was heated at 78 °C for 30 min in air atmosphere by reflux technique. The Ni adsorbed graphite was obtained through the centrifugation. In order to trigger the catalytic hydrogenation, the prepared samples were annealing in the furnace at 1000 °C for 3 h under H2 (99.999%, KOSEM) atmosphere (1000 sccm). In succession, for the formation of both the graphitic carbon shell and a-Si nanolayer on the graphite, C2H2 gas (10.0%, N2 balance, KOSEM) was flowed at 900 °C for 10 min (1000 sccm) and then SiH4 gas (99.9999%, KOSEM) was introduced into the furnace at 500 °C for 30 min (50 sccm). In case of SEAG with Ni silicide, the process of C2H2 flow was omitted.
3
Synthesis and Purification of Gold Nanoparticles
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n-Heptane (Hp) from Merck, HPLC grade, was used without further purification. Sodium 1,4-bis (2-ethylhexyl) sulfosuccinate (AOT) (Sigma greater than 99% purity) was used as received and to minimize water absorption it was kept under vacuum over P2O5. Ultrapure water was obtained from Labonco equipment model 90901-01. Tetrachloroauric acid (HAuCl4, Sigma-Aldrich) as precursor and hydrazine monohydrate (N2H4·H2O, Sigma-Aldrich) as reducing agent, both for the synthesis of Au-NPs were used as received.
4
Synthesis of δ-MnO2 Nanoplates from Mn(OH)2
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Hydrazine hydrate solution (10 mL, N2H4.H2O, 80%, Sigma-Aldrich, for synthesis) was added to 200 mL Mn(Ac)2 solution (4 mmol L−1) dropwise under constant stirring. The mixture was reacted for 5 min before being transferred into a Teflon-lined stainless-steel autoclave, and the temperature was maintained at 180 °C for 12 h. The white precipitate Mn(OH)2 was collected after water filtration three times. Mn(OH)2 (1.0 g) was weighed and dispersed with 250 mL water to form a uniform dispersion by sonicating for 10 min. Then, 50 mL of NaClO solution was added to the Mn(OH)2 dispersion dropwise under constant stirring. After that, the mixture was stirred for another 24 h, and the precipitate (δ-MnO2 nanoplates) was collected after water filtration three times and dried in a vacuum oven overnight.
5
Silver Nanoparticles Synthesis with Indolicidin
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150 μL hydrazine monohydrate (N2H4 ∙ H2O, Sigma Aldrich) were added to 1 mL AgNO3 solution (1 mM). Deionized water was added to the solution up to 2 mL, mixed for one minute and allowed to settle at room temperature for four hours. To prepare silver colloids in the presence of indolicidin, 150 μL N2H4 ∙H2O were added to 1 mL AgNO3 solution (1 mM). The solution was filled up to 2 mL with the peptide solution (indolicidin in deionized water=560 μg/mL), mixed and allowed to settle at room temperature. The nominal concentrations of the obtained solutions were 0.5 mM of AgNPs alone and 0.5 mM of AgNPs+238 μg/mL of indolicidin for the complex. We reported the concentrations for all the subsequent experiments as a function of the concentration of the peptide indolicidin. When we used AgNPs without the peptide, we used the same amount of NPs contained in the relative solution with indolicidin (for simplicity of comparisons we indicate it with the concentration value of indolicidin).
6
Synthesis of Gold Nanoparticles and Imidazolium Salts
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Tetrachloroauric acid (HAuCl4, Sigma-Aldrich) as the precursor and hydrazine monohydrate (N2H4·H2O, Sigma-Aldrich) as the reducing agent, both for the synthesis of Au–NPs were employed as received. The substrate 1,4-dinitrobenzene (DNB) from Sigma-Aldrich was employed as received. Ultrapure water was obtained from Labconco equipment model 90901-01. Imidazolium salts [1,3-bis(2,6-diisopropyl-4-sodiumsulfonatophenyl)imidazolium, 1-mesityl-3-(3-sulfonatopropyl)imidazolium and 1-(3-sulfonatopropyl)imidazolium] were prepared and characterized according to reported procedures.43,44 (link)
7
Synthesis of Tellurium Nanowires
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Typically, 1 g of PVP (MW ≈ 40 000, Anhui Sunrise Technologies) and 0.0922 g of Na2TeO3 (AR, 97%, Shanghai Aladdin Bio-Chem Technology) were added to a 50-ml Teflon lined stainless steel autoclave containing 33 ml of water. After PVP and Na2TeO3 were dissolved with vigorous magnetic stirring, 1.67 ml of N2H4·H2O (40 to 60%, w/w, Sigma-Aldrich) and 3.33 ml of NH3·H2O (25%, w/w, Shanghai Aladdin Bio-Chem Technology) were added and mixed well with magnetic stirring for 10 min. The autoclave was then sealed and kept at 180°C for 3 hours, and then gradually cooled to room temperature. To get TeNWs with larger diameters, 5 ml of acetone was added to the synthetic solution.
8
Synthesis and Characterization of Fluorescent Molecular Probes
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Rodamine 6G (99%), Coumarin 30 (99%), 4-(dimethylamino)benzaldehyde (99%), hexane (95%), chloroform (99%), ethyl acetate (99.8%), N 2 H 4 *H 2 O (98%), sodium tetrakis [3,5-bis(trifluoromethyl) phenyl]borate, sodium tetrakis[3,5-bis(1,1,1,3,3,3hexafluoro-2-methoxy-2-propyl)phenyl]borate trihydrate, silica gel (100/200 μm) were purchased from Sigma-Aldrich and used as received. All other reagents were of analytical grade and used without purification. All aqueous solutions were prepared using deionized water.
The Fourier transform infrared radiation (FT-IR) spectra of the compounds in the range 400-4000 cm -1 were recorded using a Perkin Elmer Spectrum 100BX II spectrometer in KBr pellets. 1 H, 13 C NMR spectra were performed on a Bruker Avance 400 with the frequency of proton resonance 400 MHz using CDCl 3 as the solvent and tetramethylsiliane as the internal reference. UV-VIS and fluorescence measurements were performed on Shimadzu UV-2600 spectrophotometer and Shimadzu RF-6000 spectrofluorophotometer using 1 cm path length cuvettes at room temperature. The size and electrokinetic potential of fluorescent particles were determined using ZetaSizer Nano ZS analyzer (Malvern Instruments Ltd.) The pH measurements were carried out using a Sartorius Professional Meter PP-50.
The Fourier transform infrared radiation (FT-IR) spectra of the compounds in the range 400-4000 cm -1 were recorded using a Perkin Elmer Spectrum 100BX II spectrometer in KBr pellets. 1 H, 13 C NMR spectra were performed on a Bruker Avance 400 with the frequency of proton resonance 400 MHz using CDCl 3 as the solvent and tetramethylsiliane as the internal reference. UV-VIS and fluorescence measurements were performed on Shimadzu UV-2600 spectrophotometer and Shimadzu RF-6000 spectrofluorophotometer using 1 cm path length cuvettes at room temperature. The size and electrokinetic potential of fluorescent particles were determined using ZetaSizer Nano ZS analyzer (Malvern Instruments Ltd.) The pH measurements were carried out using a Sartorius Professional Meter PP-50.
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