DHLFs were purchased from Lonza (Basel, Switzerland) and cultured in fibroblast growth medium (FBM, Lonza, Walkersville, MD, USA). Recombinant human TGFβ and PDGF were obtained from Peprotech (Rocky Hill, CT, USA) and used at a final concentration of 5 ng/ml. Chemically synthesized ONG41008 was obtained from Syngene International Ltd. (Bangalore, India), dissolved at a stock concentration of 50 mM in DMSO, and stored in aliquots at −20 °C. DMSO was used as a control. The RAW264.7 cell line was purchased from the Korean Cell Line Bank (Seoul, Korea) and cultured in RPMI supplemented with 10% FBS and 1% P/S (Welgene, Seoul, Korea). LPS was purchased from Sigma and used at a final concentration of 100 ng/mL.
Dhlfs
Manufactured by Lonza
Sourced in Switzerland
DHLFs are laboratory equipment used for handling and processing cell cultures. They provide a controlled environment for the growth and maintenance of cells, tissues, or other biological samples. The core function of DHLFs is to maintain optimal conditions, such as temperature, humidity, and gas composition, to support the viability and growth of cell cultures.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human tgf β, by Thermo Fisher Scientific (2 mentions)
Roswell park memorial institute (rpmi), by Welgene (1 mentions)
Raw 264.7 cell line, by Korean Cell Line Bank (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Eupatilin, by Syngene (1 mentions)
2 protocols using dhlfs
1
Dermal Fibroblast Culture and Stimulation
2
Evaluation of Eupatilin's Anti-Fibrotic Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DHLFs were purchased from Lonza (Basel, Switzerland) and cultured in fibroblast growth medium (FBM, Lonza, Walkersville, MD, USA). Recombinant human TGF-β and PDGF were obtained from Peprotech (Rocky Hill, CT, USA) and used at a final concentration of 5 ng/ml. Chemically synthesized eupatilin was obtained from Syngene International Ltd. (Bangalore, India), dissolved at a stock concentration of 50 mM in DMSO, and stored in aliquots at −20 °C. DMSO at 0.1% (v/v) was used as a control. ONGE200 (hispidulin) and ONGA300 (jaceosidin) were purchased from AK Scientific (Union City, CA, USA). The remaining 32 CDs in the test set were purchased from Analyticon (Berlin, Germany). For simultaneous treatment, ONGHEPA1 cells (5.5 × 104 cells/well in 1 ml) were seeded in 24-well plates. The anti-fibrotic effect of eupatilin was examined under a microscope (EVOS, Life Technologies, Carlsbad, CA, USA).
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