Fetal bovine serum (FBS) and cell culture medium were purchased from HyClone. MG132 and kanamycin were purchased from Sigma. JNK IN8 was obtained from MedChem Express. Plasmids for overexpressing HMGA1 or GRP75 and HMGA1‐specific short hairpin RNA (shRNA) were designed and synthesized by GeneChem. Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio. A cell counting kit‐8 (CCK8) kit was purchased from Bioss. A migration assay kit was obtained from BD Biosciences. Cell invasion assay chambers (24 wells) were purchased from Corning. Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P‐JNK (Thr183/Tyr185), c‐JUN, P‐c‐JUN (Ser63), P‐c‐JUN (Ser73), and GAPDH were purchased from Cell Signaling Technology. A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scientific.
Cell invasion assay chambers 24 wells
Manufactured by Corning
Sourced in United States
The Cell Invasion Assay Chambers (24 wells) are laboratory equipment designed to study cell migration and invasion in vitro. The chambers provide a controlled environment for monitoring the ability of cells to migrate through a simulated extracellular matrix barrier.
Automatically generated - may contain errors
Lab products found in correlation
Migration assay kit, by BD (4 mentions)
Small interfering rna sirna oligonucleotides, by RiboBio (4 mentions)
Mg132, by Merck Group (4 mentions)
Kanamycin, by Merck Group (4 mentions)
Gapdh, by Cell Signaling Technology (4 mentions)
Ubiquitin, by Cell Signaling Technology (4 mentions)
Jnk in 8, by MedChemExpress (4 mentions)
P c jun ser63, by Cell Signaling Technology (3 mentions)
P c jun ser73, by Cell Signaling Technology (3 mentions)
Cycloheximide (chx), by Cell Signaling Technology (1 mentions)
Pierce co immunoprecipitation kit, by Thermo Fisher Scientific (1 mentions)
Short hairpin rna shrna, by Genechem (1 mentions)
4 protocols using cell invasion assay chambers 24 wells
1
Investigating HMGA1-GRP75 Interaction in Cancer Progression
2
Inhibition of HMGA1 and GRP75 in Cancer
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+ Expand
Fetal bovine serum (FBS) and cell culture medium were purchased from HyClone (South Logan, UT, USA). MG132 and kanamycin were purchased from Sigma (St. Louis, MO, USA). JNKIN8 was obtained from MedChem Express (Shanghai, China). Plasmids for overexpressing HMGA1 or GRP75 and HMGA1speci c short hairpin RNA (shRNA) were designed and synthesized by GeneChem (Shanghai, China).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
3
Inhibition of HMGA1 and GRP75 in Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal bovine serum (FBS) and cell culture medium were purchased from HyClone (South Logan, UT, USA). MG132 and kanamycin were purchased from Sigma (St. Louis, MO, USA). JNKIN8 was obtained from MedChem Express (Shanghai, China). Plasmids for overexpressing HMGA1 or GRP75 and HMGA1speci c short hairpin RNA (shRNA) were designed and synthesized by GeneChem (Shanghai, China).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
4
Investigating HMGA1 and GRP75 Interactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal bovine serum (FBS) and cell culture medium were purchased from HyClone (South Logan, UT, USA). MG132 and kanamycin were purchased from Sigma (St. Louis, MO, USA). JNK IN8 was obtained from MedChem Express (Shanghai, China). Plasmids for overexpressing HMGA1 or GRP75 and HMGA1speci c short hairpin RNA (shRNA) were designed and synthesized by GeneChem (Shanghai, China).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
Small interfering RNA (siRNA) oligonucleotides targeting HMGA1 or GRP75 were obtained from RiboBio (Guangzhou, China). A cell counting kit-8 (CCK8) kit was purchased from Bioss (Beijing, China). A migration assay kit was obtained from BD Biosciences (Franklin Lakes, NJ, USA). Cell invasion assay chambers (24 wells) were purchased from Corning (NY, USA). Cycloheximide (CHX) and primary antibodies against HMGA1, GRP75, Ki67, Ubiquitin, JNK, P-JNK (Thr183/Tyr185), c-JUN, P-c-JUN (Ser63) and P-c-JUN (Ser73) and GAPDH were purchased from Cell Signaling Technology (Danvers, MA, USA). A Pierce coimmunoprecipitation kit was obtained from Thermo Fisher Scienti c (Waltham, MA, USA).
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