Human ifn β elisa kit

Manufactured by PBL Assay Science

Sourced in United States

The Human IFN-β ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interferon beta (IFN-β) levels in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Hcytmag 60 k px30, by Merck Group (1 mentions) Milliplex map human cytokine chemokine magnetic bead panel, by Merck Group (1 mentions) Il 18 human elisa kit, by Thermo Fisher Scientific (1 mentions) Human il 6 duoset elisa, by R&D Systems (1 mentions) Mouse ifn β elisa kit, by PBL Assay Science (1 mentions) Human il 29 ifn λ1 duoset elisa kit, by R&D Systems (1 mentions) Pierce protein concentrator, by Thermo Fisher Scientific (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Takara Bio (1 mentions) Ifn α, by Thermo Fisher Scientific (1 mentions) Poly 1 c, by Thermo Fisher Scientific (1 mentions) Rabbit anti tbk1, by Abcam (1 mentions) Rabbit anti ptbk1, by Abcam (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Rabbit anti lc3, by Merck Group (1 mentions) Rabbit anti irf3, by Santa Cruz Biotechnology (1 mentions) Mouse anti gfp, by Santa Cruz Biotechnology (1 mentions) Odyssey imager, by LI COR (1 mentions)

Spelling variants of this product

IFN-β (135 citations) Human IFN-β (18 citations) VeriKine human IFN-β ELISA kit (11 citations) IFN-β ELISA kit (9 citations) VeriKine-HS Human IFN-β ELISA Kit (2 citations)

11 protocols using human ifn β elisa kit

Quantifying Cytokine Secretion After Viral Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture supernatants were collected 12 h after H1N1 virus infection. Secreted IFN-α and IFN-β levels in the cell supernatants were determined with human Interferon-α ELISA kit (Dakewe, Shenzhen, China) and human IFN-β ELISA kit (PBL interferon source, U.S.A.).

Zhang F., Lin X., Yang X., Lu G., Zhang Q, & Zhang C. (2019). MicroRNA-132-3p suppresses type I IFN response through targeting IRF1 to facilitate H1N1 influenza A virus infection. Bioscience Reports, 39(12), BSR20192769.

+ Open protocol

+ Expand

Quantifying IFN-β Concentration in Cell Supernatants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture supernatants were harvested 24 h after treatment and used directly for measurement of IFN-β concentration. IFN-β concentrations were determined using human IFN-β ELISA kit (PBL Assay Science, Piscataway, NJ, USA).

Lin C.J., Lin H.J., Chen T.H., Hsu Y.A., Liu C.S., Hwang G.Y, & Wan L. (2015). Polygonum cuspidatum and Its Active Components Inhibit Replication of the Influenza Virus through Toll-Like Receptor 9-Induced Interferon Beta Expression. PLoS ONE, 10(2), e0117602.

+ Open protocol

+ Expand

Poly I:C Stimulation of OSM-Primed Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Subconfluent cultures (70–80%) were left untreated or pre‐incubated for 2 hrs with 20 ng/ml OSM before stimulation with 5 or 100 μg/ml poly I:C for 2 hrs. Supernatants were collected, concentrated and analysed by ELISA. The human IFN‐β ELISA kit was purchased from PBL Assay Science, Piscataway, NJ, USA (# 41410) and used according to the manufacturer's protocol.

Hergovits S., Mais C., Haan C., Costa‐Pereira A.P, & Hermanns H.M. (2017). Oncostatin M induces RIG‐I and MDA5 expression and enhances the double‐stranded RNA response in fibroblasts. Journal of Cellular and Molecular Medicine, 21(11), 3087-3099.

+ Open protocol

+ Expand

Cytokine Profiling in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma collected by Ficoll isolation from heparinized whole blood was clarified by centrifugation. Circulating cytokine levels were determined in magnetic Luminex assays with the Bio-Plex Pro Human Inflammation (BioRad 171al001m) and the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore Sigma HCYTMAG-60K-PX30) according to the manufacturer’s protocol.
ELISAs were performed according to the manufacturer’s protocols. Human IFN-β ELISA Kit (PBL Assay Science 41410-2); Human IL-6 DuoSet ELISA (R&D Systems DY206-05); IL-18 Human ELISA Kit (Thermo Fisher BMS267-2).

Taft J., Markson M., Legarda D., Patel R., Chan M., Malle L., Richardson A., Gruber C., Martín-Fernández M., Mancini G.M., van Laar J.A., van Pelt P., Buta S., Wokke B.H., Sabli I.K., Sancho-Shimizu V., Chavan P.P., Schnappauf O., Khubchandani R., Cüceoğlu M.K., Özen S., Kastner D., Ting A.T., Aksentijevich I., Hollink I.H, & Bogunovic D. (2021). Human TBK1 Deficiency Leads to Autoinflammation Driven by TNF-Induced Cell Death. Cell, 184(17), 4447-4463.e20.

+ Open protocol

+ Expand

Human and Mouse IFNβ ELISA Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human IFNβ ELISA kit (PBL Assay Science, # 41410-2) and mouse IFNβ ELISA kit (PBL Assay Science, # 42400-2) were used. The ELISA assays were performed according to the manufacturer’s protocols.

Wang L., Song K., Hao W., Wu Y., Patil G., Hua F., Sun Y., Huang C., Ritchey J., Jones C., Liu L., Guan J.L, & Li S. (2021). FIP200 restricts RNA virus infection by facilitating RIG-I activation. Communications Biology, 4, 921.

+ Open protocol

+ Expand

Quantifying IFN-λ1 and IFN-β in HIV Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

IFN-λ1 and IFN-β concentrations in the supernatants of mock, HIV-infected, and/or IFN-treated HCs (500,000 cells per condition) and accompanying controls were assessed in duplicate using the Human IL-29/IFN-λ1 DuoSet ELISA Kit (DY7246; R&D Systems, Minneapolis, MN, USA) and the Human IFN-β ELISA Kit (41410; PBL, Piscataway, NY, USA) according to the manufacturers’ instructions. Plates were read on a SpectraMax 250 plate reader and concentrations were determined by comparison to a standard curve.

Johnson E.L., Swieboda D., Olivier A., Enninga E.A, & Chakraborty R. (2021). Robust innate immune responses at the placenta during early gestation may limit in utero HIV transmission. PLoS Pathogens, 17(8), e1009860.

+ Open protocol

+ Expand

Quantifying IFNα and IFNβ in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To detect IFNα and IFNβ levels in medium, cells were cultured in serum free medium and treated with indicated drugs for 48 hours. Supernatant was then collected and concentrated using a Pierce protein concentrator (Thermo-Fisher). To test IFNα and IFNβ in lysates, cell and tumor lysates were extracted using RIPA buffer. Total protein concentrations were determined by Pierce BCA Protein Assay Kit (Fisher Scientific). Then, the levels of IFNα and IFNβ protein were measured by ELISA using human IFNα ELISA kit (41100) and human IFNβ ELISA kit (41440), from PBL Assay Science (Piscataway, NJ) according to the manufacturer’s protocol.

Gong K., Guo G., Panchani N., Bender M.E., Gerber D.E., Minna J.D., Fattah F., Gao B., Peyton M., Kernstine K., Mukherjee B., Burma S., Chiang C.M., Zhang S., Sathe A.A., Xing C., Dao K.H., Zhao D., Akbay E.A, & Habib A.A. (2020). EGFR inhibition triggers an adaptive response by co-opting antiviral signaling pathways in lung cancer. Nature cancer, 1(4), 394-409.

+ Open protocol

+ Expand

Measuring IFN-β Secretion in Oncolytic Virus-Infected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells in 2D culture were infected with oVSV-GFP at 1 MOI for 1 h, then washed and given fresh media. Culture media was collected at 6 h and 14 h, then stored at -80°C prior to analysis. The concentration of IFN-β was measured using a human IFN-β ELISA kit (PBL Assay Science, Piscataway, NJ).

Lee K.J., Lee S.W., Woo H.N., Cho H.M., Yu D.B., Jeong S.Y., Joo C.H., Jeong G.S, & Lee H. (2020). Real-time monitoring of oncolytic VSV properties in a novel in vitro microphysiological system containing 3D multicellular tumor spheroids. PLoS ONE, 15(7), e0235356.

+ Open protocol

+ Expand

Investigating IFN-mediated Immune Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Poly(I:C) (Invitrogen, Carlsbad, CA, USA), IFN-γ (Peprotech, Rocky Hill, NJ, USA), IFN-α (Peprotech), TRIzol Reagent (Takara, Dalian, China), Gamma Bind G Plus-Sepharose (Amersham Biosciences, GE Healthcare, Marlborough, MA, USA) and the human IFN-β ELISA kit (PBL, Piscataway, NJ, USA) were purchased from the indicated manufacturers. HEK293 and HeLa cells were obtained from the ATCC. Mouse anti-Flag (Sigma, St Louis, MO, USA), mouse anti-β-actin (Sigma), mouse anti-GFP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-LMNB1 (Proteintech, Rosemont, IL, USA), rabbit anti-IRF3 (Santa Cruz Biotechnology), rabbit anti-pIRF3 (CST, Danfoss, MA, USA), rabbit anti-VISA (Bethyl, Montgomery, TX, USA), rabbit anti-TBK1 (Abcam, Cambridge, UK), rabbit anti-pTBK1 (Abcam) and rabbit anti-LC3 (Sigma) antibodies were purchased from the indicated manufacturers. Rabbit and mouse antibodies against IFITM3 were raised against recombinant human IFITM3.

Jiang L.Q., Xia T., Hu Y.H., Sun M.S., Yan S., Lei C.Q., Shu H.B., Guo J.H, & Liu Y. (2017). IFITM3 inhibits virus-triggered induction of type I interferon by mediating autophagosome-dependent degradation of IRF3. Cellular and Molecular Immunology, 15(9), 858-867.

+ Open protocol

+ Expand

STING Agonist Activation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

All STING reporter cell assays were conducted according to manufacturer’s protocols. In short, CDN or control agonists were added at desired concentrations in 20 µL PBS to desired wells of flat-bottom 96-well plates. HEK-Blue, J774-Dual, THP-1 Dual, or THP-1 parental cell lines were harvested, washed, counted, and resuspended for addition of 5×10⁴ – 1×10⁵ cells per well in 180 µL recommended media. Cells were incubated with CDN for 20–24 hours, when supernatants were harvested for Luciferase or SEAP assays using QuantiLuc or QuantiBlue solutions according to manufacturer’s instructions. For ELISA, THP-1 parental cells were incubated with CDN for 72 hours, then supernatants were harvested, and IFNβ levels were quantified using the PBL Human IFNβ ELISA kit according to manufacturer’s protocols.

Ager C.R., Boda A., Rajapakshe K., Lea S.T., Di Francesco M.E., Jayaprakash P., Slay R.B., Morrow B., Prasad R., Dean M.A., Duffy C.R., Coarfa C., Jones P, & Curran M.A. (2021). High potency STING agonists engage unique myeloid pathways to reverse pancreatic cancer immune privilege. Journal for Immunotherapy of Cancer, 9(8), e003246.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!