Mouse monoclonal anti α tubulin fitc antibody

All the chemicals and reagents were purchased from Sigma unless stated otherwise. Rabbit polyclonal anti-Pak2 antibody (Cat#: ab76293) and anti-γ-H2AX (phosphor S139) antibody (Cat#: ab81299) were obtained from Abcam (Cambridge, MA, USA); mouse monoclonal anti-α-tubulin-FITC antibody (Cat#: F2168) from Sigma (St. Louis, MO, USA); Alexa Fluor 488 goat anti-rabbit IgG (H + L) (Cat#: A11008) and CM-H2DCFDA (Cat#: C6827) from Thermo Fisher Scientific Life Technologies (Massachusetts, MA, USA); an In Situ Cell Death Detection Kit (Cat#:11684817910) was purchased from Roche (Basel, Switzerland); and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (Cat#:SA00001–2), HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (Cat#:SA00001–1), and GAPDH Monoclonal Antibody (Cat#:60004-1 g) were purchased from Proteintech (Wuhan, China).

Rabbit polyclonal anti-Kif2a antibody used in Western blot and immunofluorescence was purchased from Novus Biologicals (Littleton,CO); Mouse monoclonal anti-α-tubulin-FITC antibody and mouse monoclonal anti-γ-tubulin antibody were obtained from Sigma-Aldrich Co. (Cat# F2168, Ca#T6557); Rabbit polyclonal anti-bub3 antibody was obtained from Santa Cruz Biotechology (Ca#sc-28258); Human polyclonal anti-centromere antibody (ACA) was obtained from Antibodies Incorporated (Item # 15-234-0001). Alexa Fluor@ 488-conjugate Goat anti-Rabbit IgG (H + L) and Alexa Fluor @594-conjugate Goat anti-Rabbit IgG (H + L) were produced by Thermo Fisher Scientific (Catalog# A-11008, Catalog# A-11012); Cy5-conjugated goat anti-human IgG and Cy5-conjugated goat anti-mouse IgG were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA) (Cat#115-175-146, Cat#115-175-062). Mouse monoclonal anti-β-actin antibody was purchased from Santa Cruz Biotechnology (SC-8432, Santa Cruz, CA).
All other reagents were purchased from Sigma Aldrich except when mentioned otherwise.

Rabbit polyclonal anti-CK2 antibody, rabbit polyclonal anti-Gapdh antibody, and rabbit monoclonal anti-γH2A.X antibody were purchased from Cell Signaling Technology (Danvers, MA, USA); Mouse monoclonal anti-α-tubulin-FITC antibody and anti-acetyl-α-tubulin (K40) antibody were purchased from Sigma (St. Louis, MO, USA); Rabbit polyclonal anti-phosphoCK2 and sheep polyclonal anti-BubR1 antibodies were obtained from Abcam (Cambridge, MA, USA); goat anti-mouse IgG (H + L)-FITC, goat anti-rabbit IgG (H + L)-FITC and donkey anti-sheep IgG (H + L)-FITC were obtained from Zhongshan Golden Bridge Biotechnology (Beijing, China).

All chemicals and culture media were purchased from Sigma (St Louis, MO) unless those specifically mentioned. Rabbit polyclonal anti-OLA1 antibody (Cat# A4673) was purchased from ABclonal (Wuhan, China). Mouse monoclonal anti- α-tubulin-FITC antibody (Cat# F2168) was obtained from Sigma (St Louis, MO); Sheep polyclonal anti-BubR1 antibody (Cat# 28193) was obtained from Abcam (Cambridge, UK). FITC-conjugated donkey anti-sheep IgG (H + L) was purchased from Jackson ImmunoResearch Laboratory.

Rabbit anti-ABCE1 monoclonal antibody (Cat# 185548) and sheep anti-BubR1 polyclonal antibody (Cat# 28193) were obtained from Abcam (Cambridge, UK); rabbit anti-phospho-p44/42 MAPK monoclonal antibody (Cat# 4370) was purchased from CST (Danvers, MA); mouse anti-γ-tubulin monoclonal antibody (Cat# 17787) was purchased from Santa Cruz (Santa Cruz, CA); mouse monoclonal anti-α-tubulin-FITC antibody (Cat# F2168) was obtained from Sigma (St Louis, MO). FITC-conjugated donkey anti-sheep IgG (H + L) was produced by Jackson ImmunoResearch Laboratories, Inc. Cy3-conjugated goat anti-rabbit IgG (H + L) and Cy3-conjugated goat anti-mouse IgG (H + L) were purchased from Boster Biotechnology Co., LTD (wuhan, China)
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Spindle perturbation drug nocodazole was 10 mg/ml in DMSO stock (−20°C), was purchased from Sigma-Aldrich Co. All other reagents were purchased from Sigma Aldrich unless specifically stated otherwise.

Mouse monoclonal anti-Rab6a (Cat# ab55660) and GM130 antibody (Cat# ab52649) were purchased from Abcam (Cambridge, MA, USA); Mouse monoclonal anti-α-tubulin-FITC antibody (Cat# 76074), Phalloidin-FITC (Cat# P5282) and Lectin-FITC (Cat# L4265) were purchased from Sigma (St. Louis, MO, USA); FITC-conjugated goat anti-rabbit IgG was purchased from Thermo Fisher Scientific (Rockford, IL, USA). ER-tracker red (Cat# C1041-1) was purchased from Beyotime, Fluo-4 AM (Cat# F14201), and Pluronic F-127 (Cat# P-3000MP) were purchased from Thermo Fisher Scientific.

Mouse monoclonal anti-α-tubulin FITC antibody, anti-phalloidin-TRITC antibody, anti-acetyl-α-tubulin (Lys-40) antibody and lens culinaris agglu-tinin (LCA)-FITC were purchased from Sigma (St. Louis, MO, USA); rabbit polyclonal anti-human ovastacin antibody was obtained from Dr. Jurrien Dean (NIH); FITC-conjugated goat anti-mouse IgG (H + L) and TRITC-conjugated goat anti-mouse IgG (H + L) were purchased from Zhongshan Golden Bridge Biotechnology Co., LTD (Beijing, China).