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2 protocols using goat anti rabbit igg h l alexafluor647 conjugate

1

Antibody Panel for Immune Cell Profiling

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Rabbit anti-phospho-STAT5 (RRID:AB_823649), rabbit anti-CISH (RRID:AB_11178524) and rabbit polyclonal anti-TFEB (RRID:AB_11220225) were all bought from Cell Signaling Technology, Leiden, The Netherlands. Phalloidin-iFluor 647 and Phalloidin-iFluor 405 were obtained from Abcam, Cambridge, United Kingdom. Goat anti-rabbit IgG (H + L) AlexaFluor647 conjugate (RRID:AB_2536101) was purchased from ThermoFisher Scientific, Breda, The Netherlands.. Anti-human CD11b-PE (RRID:AB_395789) and anti-human CD1a-BV605 (RRID:AB_2741933) were acquired from BD BioSciences, Vianen, The Netherlands and anti-human CD14-FITC (RRID:AB_830677) and anti-human CD163-AF647 (RRID:AB_2563475) from BioLegend, San Diego, CA, USA.
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2

Cell-based Assay with Pharmacological Modulators

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Tamoxifen citrate (tamoxifen) and rifampicin were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands). Isoniazid was purchased from SelleckChem (Munich, Germany). Gentamicin sulfate was bought from Lonza BioWhittaker (Basel, Switzerland), and hygromycin B was acquired from Life Technologies-Invitrogen (Bleiswijk, The Netherlands). Fulvestrant, 17β-estradiol, and Hoechst 33342 were purchased from Sigma-Aldrich. Rabbit polyclonal anti-TFEB (RRID:AB_11220225) was purhcased from Cell Signaling Technology (Leiden, The Netherlands). Phalloidin-iFluor 405 was obtained from Abcam (Cambridge, UK). Goat anti-rabbit IgG (H+L) AlexaFluor647 conjugate (RRID:AB_2536101) was purchased from Thermo Fisher Scientific (Breda, The Netherlands). All compounds, except Gentamicin sulfate and hygromycin B, were dissolved in 100% dimethyl sulfoxide (DMSO; Sigma-Aldrich) in stock concentrations of 10 mM, aliquoted, and kept at −80°C.
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