Log In Sign up
The largest database of trusted experimental protocols

Mtt cell growth kit

Manufactured by Merck Group
Visit Supplier
Sourced in United States

The MTT Cell Growth Kit is a lab equipment product that measures cell viability and proliferation. It utilizes the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, a colorimetric method for assessing cell metabolic activity.

Automatically generated - may contain errors

Lab products found in correlation

Tgf β, by R&D Systems (2 mentions) Elx800, by Agilent Technologies (1 mentions) Microplate reader, by Bio-Rad (1 mentions) Picogreen dsdna quantitation kit, by Thermo Fisher Scientific (1 mentions) Qiaamp dna isolation kit, by Qiagen (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions) Prohibitin, by Santa Cruz Biotechnology (1 mentions) Bcl 2, by Santa Cruz Biotechnology (1 mentions) 96 well plate, by Corning (1 mentions) Imark microplate absorbance reader, by Bio-Rad (1 mentions) Microplate reader, by Merck Group (1 mentions)

Spelling variants of this product

MTT kit (150 citations) MTT Cell Growth Assay Kit (39 citations) Cell Growth Determination Kit MTT based (17 citations) MTT Cell Growth Assay Kit CT-02 (2 citations) MTT Cell Growth Determination Kit (2 citations)

13 protocols using mtt cell growth kit

1

Isolation and Characterization of Primary Lung Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
To isolate primary lung fibroblasts, lungs were removed under aseptic conditions and minced with sterile scissors, then transferred to T175 cell culture flasks in complete DMEM. Cells were incubated for 2 wk, and media were changed every 3–4 d.
For proliferation assays, the MTT cell growth kit (Millipore-Sigma) was used. Fibroblasts were plated at 25,000 cells per well in 96-well plates in complete RPMI or conditioned media (CM) (supernatant from 4-h neutrophil cultures + 10% FBS + 1% l-glutamine + 1% penicillin/streptomycin + 0.1% amphotericin). Cells were incubated for 48 h. After 24 h, MTT reagent was added, and after 48 h, absorbance was read at 570 nm.
For gene expression, cells were plated at 250,000 cells per well in 12-well plates in CM or complete RPMI containing 4 ng/ml TGF-β (R&D Systems, Minneapolis, MN) for 24 h, after which media were removed, and cells were lysed in TRIzol for RNA isolation.
Shweiki D., Neeman M., Itin A, & Keshet E. (1995). Induction of vascular endothelial growth factor expression by hypoxia and by glucose deficiency in multicell spheroids: implications for tumor angiogenesis.. Proceedings of the National Academy of Sciences of the United States of America, 92(3), 768-772.
+ Open protocol
+ Expand
2

Isolation and Characterization of Primary Lung Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
To isolate primary lung fibroblasts, lungs were removed under aseptic conditions and minced with sterile scissors, then transferred to T175 cell culture flasks in complete DMEM. Cells were incubated for 2 wk, and media were changed every 3–4 d.
For proliferation assays, the MTT cell growth kit (Millipore-Sigma) was used. Fibroblasts were plated at 25,000 cells per well in 96-well plates in complete RPMI or conditioned media (CM) (supernatant from 4-h neutrophil cultures + 10% FBS + 1% l-glutamine + 1% penicillin/streptomycin + 0.1% amphotericin). Cells were incubated for 48 h. After 24 h, MTT reagent was added, and after 48 h, absorbance was read at 570 nm.
For gene expression, cells were plated at 250,000 cells per well in 12-well plates in CM or complete RPMI containing 4 ng/ml TGF-β (R&D Systems, Minneapolis, MN) for 24 h, after which media were removed, and cells were lysed in TRIzol for RNA isolation.
Warheit-Niemi H.I., Huizinga G.P., Edwards S.J., Wang Y., Murray S.K., O’Dwyer D.N, & Moore B.B. (2022). Fibrotic Lung Disease Alters Neutrophil Trafficking and Promotes Neutrophil Elastase and Extracellular Trap Release. ImmunoHorizons, 6(12), 817-834.
+ Open protocol
+ Expand
3

Evaluating hPDLSCs Metabolic Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
The metabolic activity of treated hPDLSCs (ATCC, Manassas, VA, USA) was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT Cell Growth Kit, Chemicon, Rosemont, IL, USA). The third generation hPDLSCs were cultured in 96-well plates (1 × 103 cells/well) for 24 h in a complete medium. Subsequently, the medium was replaced by the diluted eluates (100 μL/well) prepared previously. The control group’s cells were cultured in the medium without eluates. At 24 and 48 h, 10 μL of MTT was added to each well and cells were continuously cultured for 4 h. Then, the supernatant was aspirated and 100 μL of dimethyl sulfoxide was added. A microplate reader (VersaMax Microplate reader, Sunnyvale, CA, USA) was used to measure the absorbance at 490 nm. The relative proliferation rate was calculated as (assay group/control group) * 100%.
Liu M., He L., Wang H., Su W, & Li H. (2021). Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials. Journal of Materials Science. Materials in Medicine, 32(5), 52.
+ Open protocol
+ Expand
4

Cytotoxicity Evaluation of Dental Stem Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The cytotoxicity of the extracts to the stem cells was assessed using the MTT assay (MTT Cell Growth Kit, Chemicon, Rosemont, IL, USA), as reported previously25 (link). Briefly, hDPSCs were seeded at 1 × 104 cells/well in a volume of 180 μL DMEM medium w/o phenol red in 96-well plates. Previously, hDPSCs were starved for 24 h in serum-free culture medium at 37 °C and subsequently exposed the different bleaching eluates. Cells cultured in Alpha-MEM medium plus 10% FBS served as positive control. A final concentration of 1 mg/mL MTT was added after 24, 48 and 72 h of culture and incubated for 4 h. Finally, the MTT-containing medium was removed, and 100 µL of dimethyl sulfoxide (DMSO) was added to solubilize formazan. Absorbance at 570 nm (Abs570) was determined, using Abs690 as the reference wavelength. Each condition was performed in triplicate.
Llena C., Collado-González M., García-Bernal D., Oñate-Sánchez R.E., Martínez C.M., Moraleda J.M., Rodríguez-Lozano F.J, & Forner L. (2019). Comparison of diffusion, cytotoxicity and tissue inflammatory reactions of four commercial bleaching products against human dental pulp stem cells. Scientific Reports, 9, 7743.
+ Open protocol
+ Expand
5

Evaluating hDPSCs Metabolic Activity in Bleaching Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols
The metabolic activity of hDPSCs growing in the presence of different bleaching extracts was evaluated using the MTT assay (MTT Cell Growth Kit, Chemicon, Rosemont, IL, USA). hDPSCs were initially loaded with 1 × 103 cells per well and a volume of 180 μL in 96-well plates. Cells were starved for 24 h in serum-free medium at 37 °C in a humidified 5% CO2 atmosphere prior to testing. The serum-free medium was replaced with different material elutes. Cells cultured in α-MEM medium containing 10% FBS were the negative control. After 24, 48 and 72 h, MTT (1 mg/mL in CCM) was added after cell seeding and incubated for 4 h at 37 °C and 5% CO2. The MTT insoluble formazan was then dissolved by means of dimethyl sulfoxide DMSO that was applied for 2–4 h at 37 °C. The optical density (OD) was measured against blank (DMSO) at a wavelength of 570 nm (Abs570) and a reference filter of 690 nm by an automatic microplate reader (ELx800; Bio-Tek Instruments, Winooski, VT, USA)
Llena C., Collado-González M., Tomás-Catalá C.J., García-Bernal D., Oñate-Sánchez R.E., Rodríguez-Lozano F.J, & Forner L. (2018). Human Dental Pulp Stem Cells Exhibit Different Biological Behaviours in Response to Commercial Bleaching Products. Materials, 11(7), 1098.
+ Open protocol
+ Expand
6

MTT Assay for Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols
The MTT Cell Growth Kit (Chemicon, Temecula, CA, USA) was used to evaluate cellular viability according to the manufacturer’s instructions. Briefly, HUVECs were seeded at a density of 1 × 105 cells/mL in 100 μL of medium per well of a 96-well microplate. Different concentrations of murrangatin and/or CM were added for 24 h. Then, 10 μL of 0.5 mg/mL MTT labeling reagent was added to each well for an additional 4 h incubation at 37°C with 5% CO2 and 95% air (v/v) at 90% humidity. The purple formazan crystals produced were dissolved with 100 μL of solubilization reagent per well for further quantitative measurement at an absorbance of 570 nm using a microplate spectrophotometer.
Long W., Wang M., Luo X., Huang G, & Chen J. (2018). Murrangatin suppresses angiogenesis induced by tumor cell–derived media and inhibits AKT activation in zebrafish and endothelial cells. Drug Design, Development and Therapy, 12, 3107-3115.
+ Open protocol
+ Expand
7

Hydrogel Dose-Response Cytotoxicity and Osteogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
A dose-response relationship was assessed by diluting the tested hydrogels with complete culture medium (DMEM/F12 + 10% FBS) to achieve 4 concentrations of each hydrogel (25%, 50%, 75% and neat or 100%). These different concentrations were cultured with BMMSCs to test their cytotoxicity by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) assay (MTT Cell Growth Kit; Chemicon, Rosemont, IL). To test the osteogenic potential of the different hydrogel concentrations; COL-I gene expression was assessed using RT-qPCR and ALP activity was assessed using Enzyme-linked immunosorbent assays (ELISAs). Cells seeded in complete culture medium were used as controls.
Sultan N, & Jayash S.N. (2023). Evaluation of osteogenic potential of demineralized dentin matrix hydrogel for bone formation. BMC Oral Health, 23, 247.
+ Open protocol
+ Expand
8

Cell Viability Evaluation under Doxorubicin

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell counting was performed by means of a Burker chamber, a device of manual counting of cells at the electron microscope. Both cell groups were subjected to 1 μM Doxorubicin for 24 h. Cells were counted at different time-points of fasting in order to evaluate the most suitable STS condition.
Cell viability was assessed through Trypan blue and MTT colorimetric assays. The first is based on the principle that dead cells, having a damaged cell membrane, internalize the dye and become blue. Both dead and living cells are counted by Burker Chamber.
MTT colorimetric assay was performed starving 8000 cells in a 96-well plate and subjecting them to fasting and chemotherapy treatment. The assay was performed using the MTT Cell Growth kit (Millipore) consisting of 3-(4,5-dimethylthiazol-2)-2,5-difeniltetrazolium bromide. It measures the mitochondrial enzyme activity that reduce tetrazolium salts to formazan. The coloration intensity of the obtained solution is directly proportional to cell viability.
Rizzo S., Cangemi A., Galvano A., Fanale D., Buscemi S., Ciaccio M., Russo A., Castorina S, & Bazan V. (2017). Analysis of miRNA expression profile induced by short term starvation in breast cancer cells treated with doxorubicin. Oncotarget, 8(42), 71924-71932.
+ Open protocol
+ Expand
9

MTT Assay for Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols
For 3[4,5-dimethylthiazole-2-yl]-2,5-dipheyltetrazolium bromide (MTT) assay the
MTT cell growth Kit from Millipore (CT02) was employed. The cells were plated on
a 96-well plates with a cell density of 1 × 104/well and treated in
different groups as described above and maintained in 100 µL of DMEM F12
medium + 100 units of penicillin and streptomycin + 10% fetal bovine serum
(FBS). Wells with medium without FBS was taken as a negative control. The cells
were grown for 24 hours, after that they were treated with 0.5 mM
H2O2 for 2 hours. The control cells were plated in the
same conditions without any treatments. After this 10 µL of MTT solution
(2-5-dipheyltetrazolium sodium bromide in phosphate-buffered saline [PBS]) was
added and incubated in its presence for 4 hours. After that 100 µL of
isopropanol in 0.04 N HCl was added to each well. The absorbance was read at
490 nm within 1 hour with a Bio-Rad (USA) microplate reader. Each sample was
done in triplicate wells. The cell growth curve was determined using the average
absorbance at 490 nm from triplicate samples of 3 independent experiments.
Alamro A.A., Alsulami E.A., Almutlaq M., Alghamedi A., Alokail M, & Haq S.H. (2020). Therapeutic Potential of Vitamin D and Curcumin in an In Vitro Model of Alzheimer Disease. Journal of Central Nervous System Disease, 12, 1179573520924311.
+ Open protocol
+ Expand
10

MTT Assay for Evaluating Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the MTT assay the MTT cell growth Kit from Millipore (CT02) was employed. The cells were plated on a 96well plates with a cell density of 1 × 104/well and treated with various concentration of [1,25(OH)2D3] that contains 100 μl of DMEM F12 medium +100 units of penicillin and streptomycin. Wells with culture medium without FBS was taken as negative control. The cells were grown for 24 h, after that they were treated with 0.5 mM H2O2 for 2 h. The control cells were plated in the same conditions without any treatments. After this 10 μl of MTT solution (2-5dipheyltetra sodium bromide in PBS) was added, and incubated in its presence for 4 h. 100 μl of isopropanol in 0.04 N HCl was added to each well. The absorbance was read at 530 nm within one hour. Each sample was done in triplicate wells.
AlJohri R., AlOkail M, & Haq S.H. (2018). Neuroprotective role of vitamin D in primary neuronal cortical culture. eNeurologicalSci, 14, 43-48.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!