Bx61w1 fluorescence microscope

Manufactured by Hamamatsu Photonics

The BX61W1 is a fluorescence microscope designed for imaging and analysis of fluorescently labeled samples. It features a motorized focusing system, a high-intensity mercury lamp for fluorescence excitation, and a set of optical filters to isolate specific fluorescent signals. The microscope is capable of capturing high-resolution images and is suitable for a variety of applications in life science research.

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Lab products found in correlation

Spinning disk confocal microscope, by Yokogawa (2 mentions) Csu10b, by Yokogawa (2 mentions) Orca er camera, by Hamamatsu Photonics (2 mentions) Lmp agarose, by Thermo Fisher Scientific (1 mentions) Volocity software, by PerkinElmer (1 mentions) Volocity software, by Quorum Technologies (1 mentions)

2 protocols using bx61w1 fluorescence microscope

Brain Volume Analysis in Zebrafish Larvae

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For brain volume analysis, 2 and 7 dpf larvae were embedded in low melting point (LMP) agarose (Invitrogen) and were positioned dorsal up in order to facilitate imaging the dorsal aspect of the larvae. Brain volumes were visualized using a Quorum Technologies spinning disk confocal microscope with a CSU10B (Yokogawa) spinning head mounted on an Olympus BX61W1 fluorescence microscope and connected to a Hamamatsu ORCA-ER camera. Images were acquired using Volocity software (Improvision) and analyzed using Imaris software (Bitplane).

Dzieciolowska S., Larroque A.L., Kranjec E.A., Drapeau P, & Samarut E. (2017). The larvicide pyriproxyfen blamed during the Zika virus outbreak does not cause microcephaly in zebrafish embryos. Scientific Reports, 7, 40067.

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Visualizing Larval Trunk and Spinal Cords

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Images of larval trunk and spinal cords were visualized using a Quorum Technologies spinning disk confocal microscope with CSU10B (Yokogawa) spinning head mounted on an Olympus BX61W1 fluorescence microscope and connected to a Hamamatsu ORCA-ER camera. Images were acquired using Volocity software (Quorum Technologies).

Petel Légaré V., Harji Z.A., Rampal C.J., Allard-Chamard X., Rodríguez E.C, & Armstrong G.A. (2019). Augmentation of spinal cord glutamatergic synaptic currents in zebrafish primary motoneurons expressing mutant human TARDBP (TDP-43). Scientific Reports, 9, 9122.

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