Thp 1 cell

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany, China, United Kingdom

THP-1 cells are a human monocytic cell line derived from an acute monocytic leukemia patient. They are commonly used in cell biology research, particularly in the study of inflammatory processes and innate immune responses.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

THP-1

39 protocols using thp 1 cell

Coculture of HBE and THP-1 Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

HBE cells (Chi Scientific, Jiangsu, China), a normal HBE cell line, were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco Life Technologies, USA), 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, USA).
THP-1 cells, which are human myeloid leukemia mononuclear cells, were obtained from the National Collection of Authenticated Cell Cultures. THP-1 cells were cultured in RPMI 1640 (Gibco Life Technologies, USA) with 10% FBS. Both cell lines were cultured in a humidified incubator containing 95% air and 5% CO2 at 37°C.
In the coculture system, THP-1 cells were seeded on six-well plates at a density of 1×10⁶ cells per well and treated with 10 µM PMA for 48 h to form THP-1 macrophages (THP-M cells). Then, THP-M cells were cocultured with normal, CSE-treated or let-7 mimic-transfected HBE cells seeded on 24 mm diameter inserts with 0.4 μm pores (#3412, Corning, USA). HBE cells were transfected with mimics for 48 h and treated with 5% CSE, while THP-M cells were treated with tocilizumab24 (link),25 (link) (HY-P9917, MedChemExpress, China), an anti-IL-6 receptor antibody before they were cocultured.

Liu T., Zhang Z., Shen W., Wu Y, & Bian T. (2023). MicroRNA Let-7 Induces M2 Macrophage Polarization in COPD Emphysema Through the IL-6/STAT3 Pathway. International Journal of Chronic Obstructive Pulmonary Disease, 18, 575-591.

+ Open protocol

+ Expand

THP1 Lipoprotein-αGalCer Uptake Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

THP1 cells (American Type Culture Collection), known for their constitutive LDLR expression (17 (link)), were cultured in RPMI 1640 (Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated FCS (ultra-low endotoxin, Biosera), 4 mmol/L Glutamax (Gibco, Thermo Fisher Scientific), 5 mmol/L sodium pyruvate (MilliporeSigma), and antibiotics (Gibco, Thermo Fisher Scientific). For the uptake experiments, THP1 cells were washed in PBS, and 100,000 THP1 cells were seeded in 100 μL Opti-MEM (Gibco, Thermo Fisher Scientific) on a 96-well plate (Corning). Upon preincubation with 5 μg/mL LDLR-blocking antibody for 30 minutes (AF2148, R&D Systems, Bio-Techne), THP1 cells were incubated for 4 hours with 0.3 μL (6 μg/mL) or 0.03 μL (0.6 μg/mL) lipoprotein-αGalCer complexes per well, respectively, containing 100 ng and 10 ng αGalCer. Finally, loading of the DyLight 633–lipoprotein and AF488-αGalCer was assessed on a FACSCanto II flow cytometer (BD Biosciences) with FACSDiva software (BD Biosciences), and data were analyzed with FlowJo software v10.

Engelen S.E., Ververs F.A., Markovska A., Lagerholm B.C., Kraaijenhof J.M., Yousif L.I., Zurke Y.X., Gulersonmez C.M., Kooijman S., Goddard M., van Eijkeren R.J., Jervis P.J., Besra G.S., Haitjema S., Asselbergs F.W., Kalkhoven E., Ploegh H.L., Boes M., Cerundolo V., Hovingh G.K., Salio M., Stigter E.C., Rensen P.C., Monaco C, & Schipper H.S. (2023). Lipoproteins act as vehicles for lipid antigen delivery and activation of invariant natural killer T cells. JCI Insight, 8(9), e158089.

+ Open protocol

+ Expand

Adhesion Assay of THP-1 Monocytes to HCFs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human monocytic cell line THP-1 cells were obtained from ATCC (Manassas, VA, USA) and maintained in DMEM/F-12 medium containing 10% FBS at 37^oC. Cell adhesion assay was modified from the previous report (Liu et al., 2013 (link)). In Brief, HCFs were placed on 6-well culture plates with cover slips. The cells were pretreated with the pharmacological inhibitors for 1 h and then treated with TNF-α for another 16 h at 37^oC. THP-1 cells were maintained and suspended in DMEM/F-12 containing 10% FBS. THP-1 cells were washed, resuspended in serum-free DMEM/F-12, and incubated with BCECF/AM (10 μM, Invitrogen) for 1 h at 37°C to label the THP-1 cells. The BCECF-labeled cells were washed thrice with serum-free DMEM/F-12, resuspended in serum-free DMEM/F-12, kept in the dark at room temperature and then the labeled THP-1 cells were added to HCFs for 1 h. Gently washing the plate with serum-free DMEM/F-12 to remove the non-adherent cells, the number of adherent cells was observed by using a fluorescence microscope (ZEISS, Axiovert 200M).

Lin C.C., Yang C.C., Wang C.Y., Tseng H.C., Pan C.S., Hsiao L.D, & Yang C.M. (2016). NADPH Oxidase/ROS-Dependent VCAM-1 Induction on TNF-α-Challenged Human Cardiac Fibroblasts Enhances Monocyte Adhesion. Frontiers in Pharmacology, 6, 310.

+ Open protocol

+ Expand

Noradrenaline Treatment of THP-1 Monocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

THP-1 human monocyte cells (<6 passages) were purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured at 37 °C, 5% CO₂ in a humidified chamber. THP-1 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) [high glucose; Gibco, Gaithersburg, MD, USA] + 5% foetal calf serum (FCS) and 1% penicillin/streptomycin. For noradrenaline treatments, cells were washed and changed to starvation media (High glucose DMEM + 1.0% FCS). Cells were treated with 0, 0.1, or 1.0 μM noradrenaline (Sigma, St. Louis, MO, USA) for 48 h.

Herat L., Rudnicka C., Okada Y., Mochizuki S., Schlaich M, & Matthews V. (2017). The Metalloproteinase ADAM28 Promotes Metabolic Dysfunction in Mice. International Journal of Molecular Sciences, 18(4), 884.

+ Open protocol

+ Expand

In Vitro Inflammation Modeling with ATDC5 and THP-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mouse chondrogenic cell line ATDC5 obtained from FuHeng Cell Center (Shanghai, China). ATDC5 cell line was cultured in DMEM medium (Hyclone, Thermo Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco, Thermo Scientific) and penicillin/streptomycin solution (Solarbio Science & Technology, Beijing, China) at 37 °C in the humidified atmosphere of 5% CO₂ in air. For ATDC5 inflammation damage, the medium was replaced with medium containing 10% FBS and LPS Supplement (final concentration 10 μg/mL) for inflammatory injury of chondrocytes.
Human monocyte cell line THP-1 cells were obtained from Procell Life Science & Technology (Wuhan, China). RPMI-1640 was a suitable medium for THP-1 cells and was obtained from Gibco. THP-1 cells in FBS concentration and CO₂ incubator conditions were the same as ATDC5 cells. PMA (Phorbol 12-myristate 13-acetate, 25 ng/mL, Meilunbio, China) induced THP-1 monocytes to differentiate into macrophages. After 48 h of induction, THP-1 cells were incubated in RPMI-1640 containing LPS (final concentration 200 ng/mL) or LPS plus different HA derivates (40 μg/mL) for 24 h.

Huang H., Ding X., Xing D., Lin J., Li Z, & Lin J. (2022). Hyaluronic Acid Oligosaccharide Derivatives Alleviate Lipopolysaccharide-Induced Inflammation in ATDC5 Cells by Multiple Mechanisms. Molecules, 27(17), 5619.

+ Open protocol

+ Expand

THP-1 Cell Culture and Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols

THP-1 cells (ATCC) and related THP-1 CARD8^−/− stable cell lines were grown in Roswell Park Memorial Institute (RPMI) medium 1640 with L-glutamine and 10% fetal bovine serum (GIBCO, ThermoFisher Scientific), at 37°C and 5% CO₂. Cells were monitored for morphological features and regularly tested for mycoplasma using MycoAlert Mycoplasma Detection kit (Lonza).

Sharif H., Hollingsworth L.R., Griswold A.R., Hsiao J.C., Wang Q., Bachovchin D.A, & Wu H. (2021). Dipeptidyl Peptidase 9 Sets a Threshold for CARD8 Inflammasome Formation by Sequestering Its Active C-terminal Fragment. Immunity, 54(7), 1392-1404.e10.

+ Open protocol

+ Expand

Generation of Pro-Inflammatory Macrophages

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

RAW 264.7 cells (American Type Culture Collection [ATCC]) were cultured in DMEM (HyClone; GE Healthcare Life Science, Chicago, IL, USA) and THP-1 cells (ATCC) in RPMI 1640-GlutaMAX-I (Gibco; Life Technologies ltd, Inchinnan, UK); both media were supplemented with 10% (v/v) heat-inactivated FBS (FBSi; Invitrogen, Waltham, MA, USA) and 1% (v/v) antibiotic-antimycotic solution (AA; Invitrogen), at 37°C in 5% CO₂.
Human CD14+ monocytes were isolated from blood from healthy donors and incubated in RPMI 1640 medium with 2 mM glutamax-I/glutamine (Gibco), 10% (v/v) FBSi, and 1% (v/v) AA. To obtain monocyte-derived pro-inflammatory macrophages (M1), primary monocytes were stimulated with 5 ng/ml GM-CSF (Sigma) for 7 days at 37°C in 5% CO₂ as described previously [24 (link)].

Hansen F.C., Nadeem A., Browning K.L., Campana M., Schmidtchen A, & van der Plas M.J. (2021). Differential Internalization of Thrombin-Derived Host Defense Peptides into Monocytes and Macrophages. Journal of Innate Immunity, 14(5), 418-432.

+ Open protocol

+ Expand

Culturing Human Macrophages and THP-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human macrophages or THP-1 cells were cultured under humidified 95% air/5% CO₂ at 37°C in Gibco RPMI-1640 containing l-glutamine (0.3 g/l), penicillin (50 IU/ml), streptomycin (50 μg/ml), amphotericin B (0.95 μg/ml), and human serum or FBS (10%, v/v), respectively, unless otherwise stated. THP-1 cells were purchased from the European Collection of Cell Cultures (Salisbury, UK). THP-1 cells were incubated in RPMI-1640 (2 ml per well) containing 10% (v/v) FCS with PMA (25 ng/ml) in 12-well tissue culture plates at 1 × 10⁶ cells per well for 72 h to differentiate into macrophages. The macrophages were then washed and rested for a further 24 h before treatment with LDL. Human monocyte-derived macrophages (HMDMs) were prepared from blood donated by healthy adults using Lymphoprep™ density gradient solution (Axis-Shield, Oslo, Norway) as previously described (42 (link)). Briefly, after separation from blood cells, monocytes were incubated in RPMI medium containing 0.05% (v/v) human serum in nonadherent 6-well tissue culture plates for 40 h, then transferred to ordinary 6-well tissue culture plates with RPMI with 10% (v/v) human serum for 10 to 14 days.

Ahmad F, & Leake D.S. (2018). Lysosomal oxidation of LDL alters lysosomal pH, induces senescence, and increases secretion of pro-inflammatory cytokines in human macrophages. Journal of Lipid Research, 60(1), 98-110.

+ Open protocol

+ Expand

Differentiation of THP-1 Cells into TAMs

Check if the same lab product or an alternative is used in the 5 most similar protocols

10% fetal bovine serum (FBS) was added to RPMI 1640 medium (BI, USA) when cultivating THP-1 cells (Gibco, USA), following the transfection of THP-1 cells with lentiviral vectors, including sh-NC and sh-APOC1 (GeneChem, China). sh-NC:5′-TTCTCCGAACGTGTCACGT-3′; sh-APOC1:5′-GCATCAAACAGAGTGAACTTT-3′. After two days of PMA-mediated macrophage differentiation, THP-1 cells were collected. THP-1 cells were exposed to HCT116/LOVO culture supernatant in RPMI 1640 media for 2 days in tumor-associated macrophages (TAMs) stimulation tests, which resulted in the production of TAMs.

Tang W., Liu H., Li X., Ooi T.C., Rajab N.F., Cao H, & Sharif R. (2023). Upregulation of APOC1 Promotes Colorectal Cancer Progression and Serves as a Potential Therapeutic Target Based on Bioinformatics Analysis. Journal of Oncology, 2023, 2611105.

+ Open protocol

+ Expand

Culturing THP-1 and Peritoneal Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

THP-1 cells were obtained from American Type Culture Collection (Manassas, VA). Primary peritoneal macrophages from C57BL/6 mice were isolated by peritoneal lavage with 10 ml RPMI 1640 medium (Gibco™ |Thermo Fisher Scientific).THP-1 cells and primary peritoneal macrophages were cultured in RPMI 1640 medium which was supplemented with 10% FBS (Gibco™ |Thermo Fisher Scientific) and 1% penicillin/streptomycin at 37°C in a humidified incubator of 5% CO₂.

Li J., Bai Y., Tang Y., Wang X., Cavagnaro M.J., Li L., Li Z., Zhang Y, & Shi J. (2022). A 4-Benzene-Indol Derivative Alleviates LPS-Induced Acute Lung Injury Through Inhibiting the NLRP3 Inflammasome. Frontiers in Immunology, 13, 812164.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!