5α-Dihydrotestosterone (DHT), bicalutamide (CDX), and enzalutamide were obtained from Sigma Chemical Co. (St. Louis, MO). The Ph.D.-12 peptide library was purchased from New England Biolabs (Beverly, MA). Human cell lines (PC-3, LNCaP, CWR22R, MCF-7 and 293T) were purchased from the American Type Culture Collection (Manassas, VA). AR expression plasmids, PCMV-Flag-AR and pCMX-VP16-AR, were constructed as described previously [9 (link), 33 (link)]. RIPK1 cDNA, prepared from human MCF-7 cells, was cloned into the p3xFLAG-CMV vector (Sigma Chemical Co.). Motif mutants of the RIPK1 plasmid, p3xFLAG-mt-AxxAA, were generated using the site-directed mutagenesis kit from Stratagene (La Jolla, CA). Anti-AR (C19) and anti-RIPK1 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
Ph d 12 peptide library
Manufactured by New England Biolabs
Sourced in United States
The Ph.D.-12 Peptide Library is a versatile tool for the discovery and screening of peptide-based interactions. The library consists of a diverse collection of 12-mer peptides displayed on the surface of M13 bacteriophage, providing a comprehensive representation of potential binding partners. This product is designed to facilitate the identification of novel peptide-protein or peptide-peptide interactions, enabling researchers to explore a wide range of biological applications.
Automatically generated - may contain errors
Lab products found in correlation
P3xflag cmv vector, by Merck Group (2 mentions)
Lncap, by American Type Culture Collection (2 mentions)
Enzalutamide, by Merck Group (1 mentions)
Mcf 7, by Merck Group (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Anti ar c19, by Santa Cruz Biotechnology (1 mentions)
Lncap, by Merck Group (1 mentions)
E coli er2738 host strain, by New England Biolabs (1 mentions)
96 well polystyrene microtiter plates, by Corning (1 mentions)
3 protocols using ph d 12 peptide library
1
Androgen Receptor and RIPK1 Modulation
2
Screening of Prostate Cancer Pathways
3
Biopanning of Phage Display Library
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Target proteins (folate receptor-α, programmed death 1 and programmed death-ligand 1) were dissolved in carbonate buffer pH 9.6 and coated on polystyrene 96-well microtiter plates (Corning, Inc., NY, USA) overnight at 4 °C at a concentration of 50–150 μg/mL. The next day, each well was blocked with 250 μL 3% (w/v) non-fat milk (NFM) (Sangon Biotech Co., Ltd., Shanghai) for 2 hours at 37 °C. After washing 5 times with TBS containing 0.05% (v/v) Tween 20 (TBST), 100 μL Ph.D.-12 peptide library (New England BioLabs, Inc., USA) aliquot containing 1011 phages was added to each well and the plate was incubated for 1 hour at 37 °C. After washing 10 times with TBST and 3 times with TBS to remove the unbounded phages, the target-bound phages were eluted with 150 μL 100 mM Glycine-HCl (pH 2.2) and neutralized immediately with 9 μL 2 M Tris-HCl (pH 9.1). 5 μL of eluate was used for titering and the rest was used to infect E. coli ER2738 host strain (New England BioLabs, Inc., USA) for amplification.
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