Antibodies specific to the following antigens were purchased for immunofluorescence from Abcam: Cytokeratin 14 (Cat# ab7800), p63 (Cat# ab53039), Cytokeratin 13 (Cat# ab92551), Sox2 (Cat# ab97959), and Ki67 (Cat# ab15580). From BD Biosciences: CD104 (Itgb4, Cat# 553745), CRABP2 (Cat# 560234), and CD73 (Cat# 550738). The following reagents were used for fluorescence analysis from Life Technologies: Hoechst (Cat# H21492) and Click-iT Alexa Fluor 594 EdU labeling kit (Cat# C10339). Antibodies specific to the following antigens were purchased for flow cytometric analysis from BD Biosciences: PerCP-Cy5.5 CD45 (Cat# 550994, 1:50), PE-Cy7 CD45 (Cat# 552848, 1:50), APC CD45 (Cat# 559864, 1:50), PE-Cy7 Streptavidin (Cat# 557598, 1:50), PE CD73 (Cat# 550741, 1:25), FITC CD34 (Cat# 553733, 1:25), PE CD90 (1:50), and PE CD44 (Cat# 553134, 1:25). From Biolegend: Biotin CD104 (Itgb4, Cat# 123604, 1:50), APC CD49f (Itga6, Cat# 313616, 1:50), APC-Cy7 CD326 (EpCam, Cat# 118217, 1:25), and APC CD29 (Itgb1, Cat# 102215, 1:50). From eBioscience: PE CD166 (Cat# 12-1661, 1:25). From Abcam: p75 (Cat# ab8874, 1:50). The following reagents were used for flow cytometric staining from Life Technologies: Hoechst (Cat# H21492), Sytox Blue (Cat# S34857), and Click-iT Alexa Fluor 647 EdU labeling kit (Cat# C10424). All-trans retinoic acid (atRA) was purchased from Sigma and tamoxifen was from EMD Millipore.
Cytokeratin 13
Manufactured by Abcam
Sourced in United States
Cytokeratin 13 is a type of intermediate filament protein found in the cytoplasm of certain epithelial cells. It is a member of the cytokeratin family and is involved in the structural integrity and organization of the cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
Cytokeratin 14, by Abcam (1 mentions)
Percp cy5.5 cd45, by BD (1 mentions)
Cd45 pe cy7, by BD (1 mentions)
Streptavidin pe cy7, by BD (1 mentions)
Cd73 pe, by BD (1 mentions)
Cd34 fitc, by BD (1 mentions)
Cd90 pe, by BD (1 mentions)
Cd44 pe, by BD (1 mentions)
Hoechst, by Thermo Fisher Scientific (1 mentions)
Cd45 apc, by BD (1 mentions)
Sytox blue, by Thermo Fisher Scientific (1 mentions)
Alexa flour 488, by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide (naoh), by Fujifilm (1 mentions)
Cytokeratin 14, by Fortrea (1 mentions)
Cytokeratin 4, by Abcam (1 mentions)
Cytokeratin 14, by Proteintech (1 mentions)
Cytokeratin 18, by Abcam (1 mentions)
Fibronectin, by Abcam (1 mentions)
Htert, by Abcam (1 mentions)
Eclipse e600 microscope, by Nikon (1 mentions)
3 protocols using cytokeratin 13
1
Immunophenotyping of Stem/Progenitor Cells
2
Immunodetection of Cell Proliferation and Differentiation
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NaOH was purchased from Wako Pure Chemical Industries Ltd (Tokyo, Japan).
The mouse monoclonal antibody to PCNA was from Novocastra Laboratories Ltd. (London, UK), and rabbit polyclonal antibodies to p63 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), cytokeratin 13 (Abcam, Cambridge, UK) and cytokeratin 14 (Covance, San Diego, CA, USA) were used as primary antibodies.
The secondary antibodies, Alexa Flour 488 and 568 conjugated donkey anti-mouse IgG, were from Invitrogen (San Diego, CA, USA).
The mouse monoclonal antibody to PCNA was from Novocastra Laboratories Ltd. (London, UK), and rabbit polyclonal antibodies to p63 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), cytokeratin 13 (Abcam, Cambridge, UK) and cytokeratin 14 (Covance, San Diego, CA, USA) were used as primary antibodies.
The secondary antibodies, Alexa Flour 488 and 568 conjugated donkey anti-mouse IgG, were from Invitrogen (San Diego, CA, USA).
3
Characterization of Primary and Immortalized Human Vocal Fold Cells
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We characterized morphological features and cell markers of primary hVFE, primary hVFF, and immortalized hVFE by immunocytochemistry. Cells were cultured on coated slide chambers for 7‐14 days and fixed for 15 minutes with 4% paraformaldehyde and blocked in 5% normal goat serum for 1 hour. Slides were incubated with primary antibodies at 4ºC overnight and, after subsequent washes in PBS, species‐specific fluorescence‐conjugated secondary antibodies (Life Technologies Corporation, Carlsbad, CA) were applied in the dark for 1 hour at room temperature (RT). DAPI dye provided nuclear staining. The following primary antibodies were used for immunocytochemistry: cytokeratin 4 (1:100, Abcam, Cambridge, MA), cytokeratin 13 (1:100, Abcam), cytokeratin 14 (1:50, Proteintech, Rosemont, IL), cytokeratin 18 (1:20, Abcam), collagen I (1:500, Abcam), fibronectin (1:200, Abcam), and hTERT (1:50, Abcam). Images were captured using a Nikon Eclipse E600 microscope (Nikon, Inc, Melvillin, NY) with Olympus DP71 digital camera (Tokyo, Japan).
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