H2b egfp mice

H2B-EGFP mice, Rosa26-rtTa mice and Rosa26-tdTomato mice were obtained from the Jackson Laboratory. K19^CreERT2 mice were kind gift from Guoqiang Gu and Cedric Blanpain. Both male and female mice with mixed background (C57BL/6 and CBA) between the ages of 5 days and 6 months were used in the experiments. Nursing mothers of bigenic R26-rtTa-H2B-EGFP pups received doxycycline in the drinking water (2 g/l, AppliChem), supplemented with 5% sucrose. At P21 the K19^CreERT2/R26-tdTomato mice were injected intraperitoneally with 2 mg of tamoxifen in rapeseed oil. All procedures involving animals were conducted according to the guidelines approved by the Commission of Laboratory Animal Licenses at the Estonian Ministry of Agriculture (license no 25 and no 88).

NG2-dsRed mice (stock 008241) were purchased from The Jackson Laboratory. LifeAct–EGFP mice were generated previously [31 (link)], and provided by R. Wedlich-Söldner (Max-Planck Institute of Biochemistry, Martinsried, Germany) and L. M. Machesky (Beatson Institute for Cancer Research, Glasgow, UK). H2B-EGFP mice (stock 006069) were purchased from The Jackson Laboratory.
Approximately 30 mice were used to perform the described experiments. Mice were housed under the approval and the institutional guidelines governing the care of laboratory mice of the Italian Ministry of Health, under authorization number 1073/2015-pr and in compliance with the international laws and policies.