Ft ir 6300 spectrometer

A TA Instruments Q20 Differential Scanning Calorimeter (New Castle, DE, USA) was used for differential scanning calorimetry (DSC) analyses of pure curcumin, RosA, UrsA, BSA, drug formulations (Curcumin/BSA, RosA/BSA, and UrsA/BSA), and physical mixtures (PMs) of each drug with BSA. The instrument was calibrated using indium, and sample scanning was conducted under nitrogen gas at 20 mL/min. A DSC isotherm curve was generated for specimens in an aluminum pan with a lid at a temperature range of 40–300 °C (with a heat rate of 10 °C/min).
Next, in order to assess any crystalline properties of the formula, an X-ray diffractogram (XRD) was also measured for pure curcumin, RosA, UrsA, BSA, drug formulations (C/BSA, RosA/BSA, and UrsA/BSA), and physical mixtures (PMs) of drug and BSA. An Ultima IV diffractometer (Rigaku, Japan) was used with a voltage of 45 kV and a current of 30 mA for XRD patterns. The diffraction angle (2θ) of the sample was scanned between 0° and 90°. Furthermore, chemical component analysis results for each type of drug formula were obtained with an FT-IR 6300 spectrometer (JASCO, Tokyo, Japan). Potassium bromide pellets containing a single compound, physical mixtures, and formulations were used. Data were collected at a wavelength of 4000–400 cm⁻¹ at ambient temperature.

Pyridine derivatives and H₂O₂ (H0300, 35%) were purchased from Tokyo Chemical Industry and Samchun Chemicals, respectively. Poly(isobutylene-alt-maleic anhydride) (531278), and poly(maleic anhydride-alt-1-octadecene) (419117), were purchased from Sigma Aldrich. ¹H NMR and ¹³C NMR spectral measurements were obtained from a 500 MHz Agilent (Varian) VNMRS spectrometer with tetramethylsilane as the reference. FTIR spectra of the catalysts were recorded on a Jasco FT/IR-6300 Spectrometer. Selectivity and conversion yield of CPNO were determined using a Gas Chromatography/Mass Spectrometer (GC/MS, Varian-320MS).

HOB cells were seeded onto coverslips in tissue culture treated dishes at a density of 7 × 10⁴ in osteogenic medium, as described above. About 48 h later, osteogenic media was replaced with media containing either no treatment or treatments with Prostaglandin EP2 receptor agonist alone, EP1 receptor antagonist alone, BMP7 alone, DMSO vehicle alone (Ct) and combination of Prostaglandin EP2 receptor agonist with BMP7 or Prostaglandin EP1 receptor antagonist with BMP7. Subsequently, the cultures were returned to the incubator, with media and treatments refreshed every 3–4 days until reaching a 21-day culture period. On day 21, media was discarded, and the cultures were rinsed twice with 1x PBS. FT-IR data were then collected in transmittance within the mid-IR spectral region, utilizing FT-IR- 6300 spectrometer (Jasco, Japan) with a spatial resolution of 50 μm. The images derived from the infrared spectra were subjected to analysis using dedicated spectra analysis software. Evaluation of total protein, mineral contents and the collagen were assessed based on the integrated areas [30 (link)].

Thin films morphology and roughness were analyzed by atomic force microscopy-AFM (XE-100 type from Park Systems, Suwon, Korea) and scanning electron microscopy-SEM (FEI, model Inspect S50, Eindhoven, The Netherlands) Fourier transform infrared spectroscopy-FTIR (JASCO FTIR 6300 spectrometer, Vienna, Austria) working on transmission mode were used to survey especially the organic component. The structural investigations of the thin films were performed by X-Ray Diffraction at grazing incidence (GI-XRD ω = 0.25^°) on a Panalytical X’Pert MRD system (Almelo, The Netherlands) (λ_CuKα = 1.5418 Å). The crystalline structure of the primary materials was checked by X-ray Diffraction (XRD) using a Bragg-Brentano geometry on a Panalytical X’Pert MPD system (λ_CuKα = 1.5418 Å). Contact angle (CA) measurements were performed using a Contact Angle Tensiometer CAM 200 from KSV Instruments, Filderstadt, Germany).

Spectra were performed on a Jasco FT-IR 6300 spectrometer (Tokyo, Japan), in a range of 4000-650 cm⁻¹.