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Cd95 dx2

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CD95 (DX2) is a lab equipment product designed for research purposes. It is a tool used in cellular and molecular biology studies. The core function of CD95 (DX2) is to facilitate the detection and analysis of the CD95 protein, which is involved in programmed cell death (apoptosis) pathways. This product provides a specific and reliable method for researchers to investigate the role of CD95 in various biological processes.

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Lab products found in correlation

Cd4 l200, by BD (3 mentions) Cd8 sk1, by BD (2 mentions) Ki67 b56, by BD (2 mentions) Hla dr l243, by BioLegend (2 mentions) Cd3 sp34 2, by BD (2 mentions) Cd21 b ly4, by BD (2 mentions) Cd279 pd1 clone eh12.2h7, by BioLegend (1 mentions) Cd3 clone sp34 2, by BD (1 mentions) Cxcr5 mu5ubee, by Thermo Fisher Scientific (1 mentions) Cd28 cd28.2, by Thermo Fisher Scientific (1 mentions) Perforin pf 344, by Mabtech (1 mentions) Tnfα mab11, by BD (1 mentions) Il 2 mq1 17h12, by BD (1 mentions) Acculi flow cytometers, by BD (1 mentions) Igm g20 127, by BD (1 mentions) Facsverse, by BD (1 mentions) Transcription factor buffer set, by BD (1 mentions) Cd16 3g8, by BD (1 mentions) Icos c398.4a, by BioLegend (1 mentions) Pd 1 eh12.2h7, by BioLegend (1 mentions)

5 protocols using cd95 dx2

1

Multi-Marker Immune Profiling Protocol

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Antibodies used in this study are as follows: CD3 (clone SP-34–2; BD Biosciences), CXCR5 (MU5UBEE; eBioscience), GagCM9 tetramer (NIH tetramer core), CD28 (CD28.2; eBioscience), CD95 (DX2; BD Biosciences), CD279 (PD-1; clone EH12.2H7; BioLegend), CD8 (SK1; BD Bioscience), Ki67 (B56; BD Biosciences), CD4 (L200; BD Biosciences), GrzB (GB11; BD Biosciences), perforin (Pf-344; Mabtech), FoxP3 (206D; BioLegend), HLA-DR (L243; BioLegend), IFNλ (B27; BD Biosciences), TNFα (MAb11; BD Biosciences), and IL-2 (MQ1- 17H12; BD Biosciences).
Rahman S.A., Yagnik B., Bally A.P., Morrow K.N., Wang S., Vanderford T.H., Freeman G.J., Ahmed R, & Amara R.R. (2021). PD-1 blockade and vaccination provide therapeutic benefit against SIV by inducing broad and functional CD8+ T cells in lymphoid tissue. Science immunology, 6(63), eabh3034.
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2

Cytometric Profiling of PBMC Subsets

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PBMCs were labeled with a combination of the following mAbs: CD3 (SP 34-2), CD4 (L-200), CD8 (SK1), CD20 (2H7), CD21 (B-ly4), CD27 (M-T 271), CD28 (CD28.2), CD95 (DX2), and IgM (G20-127) (BD Pharmingen, San Jose, CA). CD38 (OKT10) was purchased from Nonhuman Primate Reagent Resource (Boston, MA). Phenotypic markers for memory and naive T cells in cynomolgus monkeys were chosen based on the studies by Pitcher et al (22 (link)). And also mature and immature B cells were chosen based on the studies by Liu et al (23 (link)). The fluorescence of the stained samples was analyzed using FACSverse (BD Biosciences) and Acculi flow cytometers (BD PharMingen, San Jose, CA), and FlowJo software (Tree Star, Inc., Ashland, OR).
Oura T., Hotta K., Lei J., Markmann J., Rosales I., Dehnadi A., Kawai K., Ndishabandi D., Smith R.N., Cosimi A.B, & Kawai T. (2016). Immunosuppression with CD40 Costimulatory Blockade plus Rapamycin for Simultaneous Islet and Kidney Transplantation in Nonhuman Primates. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 17(3), 646-656.
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3

Tonsillar B-cell Subset Characterization

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For characterization of tonsillar B-cell subsets, single cell suspensions were stained with appropriate combinations of fluorescently labeled mAbs, including anti-CD10 (CB-CALLA), CD19 (SJ25C1), CD20 (2H7), CD22 (4KB128 and S-HCL-1), CD27 (LG.7F9), CD38 (HB7) (eBioscience), IgD (IA6-2), CD3 (SP34-2), and CD95 (DX2) (BD Biosciences). Live cells were identified using LIVE/DEAD Fixable Near-IR staining (Molecular Probes) according to the manufacturer’s instructions. Cultured cells were pelleted, washed, and stained with LIVE/DEAD, followed by surface staining with fluorescently labeled mAbs. For Blimp1 intracellular staining, cells were first stained with LIVE/DEAD fixable dye, washed, stained with appropriate surface markers, washed and then fixed, permeabilized, and stained with PE-conjugated rat IgG2ak anti-Blimp1 Ab (6D3) using the Transcription Factor Buffer Set (BD). CFSE-labeled cells were cultured for 3 days and the levels of cell proliferation were measured based on CFSE dilution. Multicolor flow cytometry was performed using a five-laser LSRII flow cytometer (BD) and analyzed with FlowJo software (Tree Star).
Giltiay N.V., Shu G.L., Shock A, & Clark E.A. (2017). Targeting CD22 with the monoclonal antibody epratuzumab modulates human B-cell maturation and cytokine production in response to Toll-like receptor 7 (TLR7) and B-cell receptor (BCR) signaling. Arthritis Research & Therapy, 19, 91.
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4

Multiparameter Flow Cytometry of Macaque Immune Cells

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The following fluorochrome conjugated monoclonal antibodies reactive with macaque cells were used for flow cytometry studies: CD3 (SP34-2, BD Biosciences [BD]), CD4 (L200, BD), CD8 (SK1, BD), CD95 (DX2, BD), CD28 (CD28.2, BioLegend), CCR5 (3A9, BD), CXCR5 (MU5UBEE, Invitrogen), PD-1 (EH12.2H7, BioLegend), ICOS (C398.4A, BioLegend), CCR7 (150503, BD), α4β7 (A4B7, NHP Reagent Resource), LAG3 (3DS223H, eBioscience), Tim-3 (F38-2E2, BioLegend), TIGIT (MBSA43, Invitrogen), CD20 (2H7, BioLegend), CD19 (J3-119, Beckman Coulter), HLA-DR (L243, BioLegend), CD10 (HI10A, BioLegend), CD21 (B-ly4, BD), CD27 (O323, BioLegend), IgD (IADB6, Southern Biotech), IgG (G18-145, BD), IL-21R (2G1-K12, BioLegend), Ki-67 (B56, BD), BCL6 (IG191E/A8, BioLegend), CD80 (2D10, BioLegend), CD56 (B159, BD), CD45 (D058-1283, BD), CD14 (MoP9, BD), CD16 (3G8, BD), CCR2 (48607, R&D Systems), CD11b (ICRF44, BioLegend), CD11c (3.9, Invitrogen), PD-L1 (29E.2A3, BioLegend), PD-L2 (24F.10C12, BioLegend), CD80 (2D10, BioLegend), CD86 (FUN-1, BD), CD141 (1A4, BD), CD163 (GHI/61, BioLegend), and CD123 (7G3, BD).
Kvistad D., Pallikkuth S., Sirupangi T., Pahwa R., Kizhner A., Petrovas C., Villinger F, & Pahwa S. (2021). IL-21 enhances influenza vaccine responses in aged macaques with suppressed SIV infection. JCI Insight, 6(20), e150888.
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5

Lymph Node and GI Tissue Analysis

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At the indicated time points, inguinal lymph nodes were collected and flash frozen. GI biopsies from upper GI (duodenum) and lower GI (colon) were collected as described previously.29 (link) Single-cell suspensions were prepared from cell culture or peripheral tissues. Peripheral blood cell subsets were sorted using magnetic bead kits from Miltenyi Biotec (Bergisch Gladbach, Germany) or through antibody labeling and a FACSAria II machine (BD Biosciences, Franklin Lakes, NJ, USA). The following antibodies (clones) were purchased from BD Biosciences: CD3 (SP34-2), CD4 (L200), CD45 (D058-1283), CCR5 (3A9), CD11b (ICRF44), CD14 (M5E2), CD16 (3G8), CD34 (563), CD28 (CD28.2), and CD95 (DX2). Anti-CD8 (B9.11) and anti-CD19 (J3-119) were purchased from Beckman Coulter (Brea, CA, USA). Anti-CD38 (AT-1) was purchased from STEMCELL Technologies (Vancouver, BC, Canada). For surface marker measurement, individual antibodies were added to the cell suspension alone or together. Isotype control antibodies were used for gating the positive group of target cells. Cells were stained with antibodies for 20 min at room temperature followed by washing twice with PBS plus 2% FBS. After resuspension, cells were subjected to FACS analysis in less than 8 h.
Yu S., Ou Y., Xiao H., Li J., Adah D., Liu S., Zhao S., Qin L., Yao Y, & Chen X. (2020). Experimental Treatment of SIV-Infected Macaques via Autograft of CCR5-Disrupted Hematopoietic Stem and Progenitor Cells. Molecular Therapy. Methods & Clinical Development, 17, 520-531.
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