Ht 12 v4.0 platform

Total RNA was extracted from serum and cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). cDNA synthesis was carried out using a RT kit (Takara Biotechnology Ltd.). MicroRNA-363-3p expression was quantified by using SYBR Premix Ex TaqTM (Takara) under ABI 7500 Fast Sequence Detection System (Applied Biosystems Prism; Thermo Fisher Scientific, Inc.).
Microarray analysis was performed using Illumina, HT-12 v4.0 platform (Bencos Research Solutions). Total RNA was extracted from serum and cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). Gene expression analysis was performed commercially using Illumina, HT-12 v4.0 platform and expression of genes was analyzed using R 3.1.2 (www.r-project.org).

The Illumina HT-12 v. 4.0 platform was used to generate whole-genome expression data. The raw data were transformed and normalized using the variance stabilizing normalization method and probes with detection P-value of <0.01 in at least 10% of the samples were used for analysis. Data are presented as the means ± SD. A moderate permutation-based two-tailed Welch t-test was applied to estimate differences between groups. A Bonferroni-adjusted P-value <0.05 was considered to indicate a statistically significant difference. Analyses were performed using MultiExperiment Viewer Software 4.9.0.