Single-molecule fluorescence experiments were performed on a home-built Nikon Eclipse Ti-U chassis configured for total internal reflection (TIR) fluorescence using a Nikon Plan APO 100× 1.45 NA objective and a 560 nm laser source (MPB Communications). With a typical 1.4 kW/cm2 excitation illumination, the fluorescence images were filtered with an ET605/70m-2p band-pass filter (Chroma Technology Co.), and acquired on an EMCCD (iXon Ultra 897, Andor) operating at 10 frames per second. The voltage function was generated by a potentiostat (CV-27 Voltammograph) and applied on the silica-ITO working electrode with respect to an Ag/AgCl reference electrode (RE). A PCI-6251 (National Instruments) data acquisition card and a BNC-2090 breakup box were used to interface the potentiostat and the PC and to digitize the current–voltage signal.
Pci 6251
Manufactured by National Instruments
Sourced in United States
The PCI-6251 is a high-performance data acquisition (DAQ) device from National Instruments. It features 16-bit analog-to-digital conversion and can acquire data at a maximum rate of 1.25 million samples per second. The device provides multiple analog input and output channels, as well as digital input/output capabilities, making it suitable for a variety of data acquisition and control applications.
Automatically generated - may contain errors
Lab products found in correlation
Ixon ultra 897, by Oxford Instruments (1 mentions)
Eclipse ti u, by Nikon (1 mentions)
Ed1 electrostatic speaker driver, by Tucker-Davis Technologies (1 mentions)
Es1 free field electrostatic speaker, by Tucker-Davis Technologies (1 mentions)
Axopatch 200b amplifier, by Molecular Devices (1 mentions)
300c lr, by Aurora Scientific (1 mentions)
S48 stimulator, by Natus (1 mentions)
Form 2, by Formlabs (1 mentions)
Solidworks, by Dassault Systèmes (1 mentions)
Pci 6052e, by National Instruments (1 mentions)
P 2000, by Sutter Instruments (1 mentions)
14 protocols using pci 6251
1
Single-Molecule Fluorescence Electrochemistry
2
Vibrotactile Stimuli Transmission Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vibrotactile stimuli were transmitted to participants’ hand via a mobile device mockup that they were grasping (Fig 1 ). The mockup was made of acrylic resin and it was similar in size to a commercial mobile phone (11 × 6 × 1 cm). A small linear vibration actuator (Tactile Labs; Haptuator TL002-14-A; 12.5 g) generated all vibrotactile stimuli that were used in both experiments. This actuator provides stronger output over a broad bandwidth (50–500 Hz, with a weak resonance at 60 Hz) than the miniature actuators that are used in commercial mobile devices. The actuator was attached to the center of the top side of the mockup using adhesive rubber tape (Fig 1 , right). A computer controlled the actuator via a 16-bit data acquisition board (National Instruments; model PCI-6251) at a 20-kHz sampling rate and a custom amplifier that supplied sufficient power for the operation of the actuator. To measure the generated vibration amplitude, an accelerometer (Kistler; model 7894A500; 7.5 g) was attached at the center of the wide face of the mockup. The acceleration data were measured at 10 kHz. The total moving mass of the mockup, the actuator, and the accelerometer was 104.5 g.
3
Electrostatic Speaker Calibration Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The speaker used in this study was an ES1 Free Field Electrostatic Speaker driven by an ED1 Electrostatic Speaker Driver manufactured by Tucker-Davis Technologies (TDT Inc, USA). The sound pressure level (SPL) of pure tones and white noise was calibrated using a 1/4" pressure prepolarized condenser microphone system (377A01 microphone +426B03 preamplifier +480E09 signal conditioner, PCB Piezotronics Inc, USA). The signals were sampled at 1 MHz by a high-speed DAQ board PCI-6251 from National Instruments and our customized Labview program was use for calibration.
4
Calibrated Magnetic Speaker Setup for Audiological Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A free-field magnetic speaker (MF1, TDT Inc., USA) was placed 1 cm away from the left ear of the animal. The speaker was driven by a stereo power amplifier (SA1, TDT Inc., USA) and calibrated using a 1/4″ pressure microphone setup with a prepolarized condenser (377A01 microphone + 426B03 preamplifier + 480E09 signal conditioner, Piezotronics Inc., USA). The captured signals were sampled at 1 MHz/s by a high-speed DAQ board (PCI-6251, National Instruments, USA). Customized LabVIEW programs were used for calibration and sound generation.
5
Nanopore Lifetime Measurements and DNA Biosensing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For nanopore lifetime measurements, DNA samples were diluted in 4 M LiCl, 1 x TE, pH ∼ 9.4 to make a DNA carrier of a concentration 0.4 nM by mixing the same volume of DNA carrier with 8 M LiCl pH∼ 9.4 - (2 x TE, 410 μL 2 M LiOH), then further diluting in 4 M LiCl pH∼ 9.4 - (1 x TE, 205 μL 2 M LiOH). For concentration measurements, 5 nM of biosensing DNA carrier was mixed with the target strand at the desired concentration in 1x Tris-HCl (pH 8.0) and incubated at room temperature for 3 hours before measurement. For amplifying the biotin-oligo signal, monovalent streptavidin (Howarth et al., 2006 (link)) was also added to a final concentration of 10 nM and incubated for 15 minutes at room temperature.
To measure the sample, the solution was added to the loading chamber. To connect the chip, Ag/AgCl single grounded electrode is placed in the loading chamber, and another electrode was placed in an outer chamber. To drive the DNA carrier through the nanopore, a voltage of 600 mV was applied using an Axopatch 200B amplifier (Molecular Devices), then the ionic current signal was measured. An external Bessel filter (Frequency Devices) was used to filter the signal at 50 kHz and then digitized at 1 MHz sampling rate for measurement with a data card (PCI-6251, National Instruments). LabVIEW algorithms (National Instruments) were used to collect the data and analyze it.
To measure the sample, the solution was added to the loading chamber. To connect the chip, Ag/AgCl single grounded electrode is placed in the loading chamber, and another electrode was placed in an outer chamber. To drive the DNA carrier through the nanopore, a voltage of 600 mV was applied using an Axopatch 200B amplifier (Molecular Devices), then the ionic current signal was measured. An external Bessel filter (Frequency Devices) was used to filter the signal at 50 kHz and then digitized at 1 MHz sampling rate for measurement with a data card (PCI-6251, National Instruments). LabVIEW algorithms (National Instruments) were used to collect the data and analyze it.
6
Automated Auditory Stimulus Generation and Calibration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A customized LabVIEW (2014 edition, National Instruments, USA) program was used for the signal waveform generation and sound calibration. The auditory stimuli were delivered via a free-field magnetic speaker (MF1, TDT Inc., USA), which was driven by a stereo power amplifier (SA1, TDT Inc., USA). A 1/4″ pressure microphone and a prepolarized condenser (377A01 microphone + 426B03 preamplifier +480E09 signal conditioner, Piezotronics Inc., USA) were used for the sound calibration. A high-speed DAQ board (1 MHz/s sampling rate, PCI-6251, National Instruments, USA) was used to record the signals. To obtain the TRF, 568 pure tones (0–70 dB SPL, 10-dB step; 0.5–64 kHz, 0.1 octave step; 35 ms duration; 5 ms sine ramp; 250 ms inter-stimulus interval) were pseudo-randomly delivered and counted as one trial.
7
In Vivo Muscle Mechanics Imaging
8
Measuring Liquid Entry Pressure of Membranes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure the LEP of membranes, we built a custom-designed apparatus (see Supplementary Fig. 6 and Supplementary Note 5 ). The tested membrane was mounted inside a filter holder, which connected to a syringe pump. By slowly pumping the salty water into the filter holder (0.1 mL min−1), the hydrostatic pressure applied on the tested membrane gradually increased. The hydrostatic pressure was monitored using a pressure sensor (IPSLU-M12, RS-Pro) and the data was recorded using a data acquisition system (PCI 6251, National Instruments). Once the applied pressure exceeded the capillary pressure of the membrane pores, liquid penetrated the membranes, leading to a pressure drop. The obtained peak value of the pressure measurement gives the LEP of the tested membrane.
9
Ultrasonic Imaging Experimental Setup
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The measurement platform for the lensing experiment descried in the previous section is essentially a scaled-down version of the 2D scanning stage we have used for kilohertz field-mapping experiments6 (link),26 (link). As shown in Fig. 4 , an ultrasonic waveguide was fabricated with laser-cut acrylic plates to confine the transmitted ultrasonic wave in a quasi-two-dimensional space. A pair of Murata ultrasound transducers (part number MA40S4S and MA40S4R) were used as the transmitter and the receiver. Two 3D printed tapered waveguiding adapters with glass tubes (about 1 mm diameter) were used to guide the ultrasonic wave from the transmitter into the waveguide, and guide the received wave from the waveguide to the receiver transducer. A LM358-based operational amplifier was used as the pre-amplification system. The pre-amplified signal was transmitted then to and be digitized by the NI PCI-6251 data-acquisition system. A two-dimensional linear stage was programmed to scan the field along the pre-defined trajectory along the focal curve.![]()
The experimental setup of the ultrasonic imaging experiment.
10
Microneurography and sEMG Signal Recording
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microneurography and sEMG signals were recorded at a sampling rate of 10,000 Hz with a 16-bits data acquisition board (National Instruments PCI-6251), installed on a personal computer (PC) running a custom program written in Labview 2012 that handled the recording and the real-time processing assisting the neurologist in seeking the fibers. The pressure sensor was custom-made [24 ] and constituted by an airtight sphere-catheter system connected to a Programmable Interface Controller at its turn connected to the PC by a serial port. The recording of its data was performed at a sampling rate of 10 Hz and controlled by the aforementioned custom program. The sensor readout was shown in the GUI.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!