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10 protocols using benzoyl peroxide

1

Isosorbide-based Polymer Synthesis

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Isosorbide (98%) was obtained from the Hongbaifeng Industry and trade Co., Ltd. (Jinan, China). Sodium chloride, sodium bicarbonate, acrylic acid (99%, inhibited with 200 ppm mono-methyl ether hydroquinone) and methyl methacrylate (98%, inhibited with 250 ppm mono-methyl ether hydroquinone) (MMA) were bought from Aladdin Co. Shanghai, China. p-toluenesulfonic acid, chloroform, benzoyl peroxide, hydroquinone, methanol, acetone, toluene, and CDCl3 were acquired from Sinopharm Chemical Reagent Co., Ltd. Prior to the experiments, isosorbide was purified through ethyl acetate recrystallization. Methyl methacrylate was washed to remove the inhibitor by a 5% solution of sodium hydroxide. The oil phase liquid was separated and dried by anhydrous calcium chloride. Other reagents were used as received without further purification.
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2

Synthesis of Magnetic Nanocomposites for Environmental Remediation

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Standard Formic acid (FA), mefenamic acid (MA), chloroamphenicol (CP), ketoprofen (KEP), clofibric acid (CA), indometacin (IDM), acetylsalicylic acid (ASP), bisphenol A (BPA), 2-phenylphenol, gemfibrozil (GEM), triclosan (TCS), and ibuprofen (IBU) (>98% of purity for all the chemicals) were purchased from J&K Scientific (Shanghai, China). Methanol (Tedia, USA) and acetonitrile (ACN) (Tedia, USA) were HPLC-grade. Divinylbenzene (DVB, 80%), benzoyl peroxide (BPO, 98%), toluene (TOL, 99.5%), oleic acid (OA), and 1,2-dichloroethane (DCE, 99%) were provided by Sinopharm Chemical Reagent Co., Ltd. (China). Ferric chloride hexahydrate (FeCl3 • 6H2O), ferrous chloride tetrahydrate (FeCl2 • 4H2O), anhydrous ferric chloride (FeCl3), N,N-dimethyl-1,3-propanediamine, ammonium hydroxide, and other inorganic salts (analytical grade) were purchased from Nanjing Chemical Reagent Co., Ltd. (Nanjing, China). Ultrapure water prepared by the Milli-Q water purification system (Billerica, MA, USA) was used for all experiments.
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3

Synthesis and Characterization of Polyacrylate Fiber for Microbial Growth

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n-Butyl acrylate (n-BA), methyl methacrylate (MMA), 2-ethylhexyl acrylate (2-EHA), lauryl methacrylate (LMA), benzoyl peroxide (BPO), ethylene glycol dimethacrylate (EDMA, cross-linking agent), phenol, inorganic salts and analytical reagent grade were commercially obtained from Sinopharm Chemical Reagent Co., Ltd and used as received without further modification. Double-distilled water was filtered through a Millipore membrane filter before being used. The fiber substrate (polyester fiber, 505 g/m2 in weight and 2 cm in thickness) was purchased from Shanghai Yanpai Filter-cloth Co., Ltd. A pure strain of P. putida (CICC 21906) was purchased from China Center of Industrial Culture Collection. Tryptone and Yeast extract were purchased from Suzhou Biogene Biotechnology Co., Ltd.
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4

Acrylic Composite Material Fabrication

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A commercial acrylic product of polymethyl methacrylate powder was supplied by Arkema, France. Methyl methacrylate (MMA) aqueous (99.5%) and catechol (99.5%) were provided by Aladdin Reagent Co., Ltd, China. The brass microspheres and 304 stainless steel microspheres are provided by China Yiwu Yue Li Hardware Products Co., Ltd, China. The polyoxymethylene microspheres were supplied by Shangyu Yixin Balls Industry Co., Ltd, China. The diameters of the above three microspheres are 2.381 mm. The benzoyl peroxide (BPO, ≥98%, containing 28–32% water) and N,N-dimethyl-p-toluidine (Dmpt, 98%) were both supplied by Sinopharm Chemical Reagent Co., Ltd, China.
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5

Synthesis of Recycled PET Composites

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Industrial PET pellets, commercial 191-type
resin (Purity: 99%),
and Chinese fir were all purchased in the market. Waste PET recycled
materials were obtained from PET bottle recycling and homemade. The
alcoholysis agent 1,2-propanediol (PG, A.R. 99%) was purchased from
Guangdong Guanghua Technology Co., Ltd., China. The catalyst zinc
acetate dihydrate (A.R. 99%), the curing initiator benzoyl peroxide
(BPO, A.R. 99%), the inhibitor hydroquinone (HQ, A.R. 99%), and dibutyl
phthalate (A.R. 99%) were all provided by Sinopharm Group Chemical
Reagent Co., Ltd., China. The diluent monomer styrene (St, A.R. 99%)
and methyl methacrylate (MMA, A.R. 99%) were produced by Shanghai
McLean Biochemical Science Co., Ltd., China. The cross-linking agent
divinylbenzene (DVB, A.R. 80%) was purchased from Tianjin Baima Technology
Co., Ltd., China. The drugs were used directly for the experiment
without purification after purchase.
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6

Synthesis and Evaluation of Multifunctional Nanoparticles

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Folic acid (FA), β-cyclodextrin (β-CD), sodium periodate, dichloromethane (DCM), diethyl ether, tetrahydrofuran (THF), dioxane, benzoyl peroxide (BPO), triethylamine (TEA), and dimethylsulfone (DMSO) were obtained from Sinopharm Chemical Reagent Co., Ltd, China. N-hydroxysuccinimide (NHS), acryloyl chloride and p-aminoazobenzene (AZO) were purchased from Aladdin. Trypsin, Dulbecco's modified Eagle's medium (DMEM), Camptothecin (CPT) and 3-(4, 5-dimethyl) thiazol-2,5-dimethyl tetrazolium bromide (MTT) were obtained from Sigma. Fetal bovine serum (FBS) was purchased from Sijiqing biotech. Co., China. All other reagents and solvents were of analytical grade and used as received.
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7

Synthesis and Characterization of Multifunctional Polymer Composites

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1,3,5–Triformylphloroglucinol (Tp), benzidine (BD), diethylbenzene and sodium hydroxide, Alpha Chemical (Zhengzhou, China).; tritoluene, octylphenol (OP) and nonylphenol (NP), Aladdin (Shanghai, China); bisphenol A (BPA), Shanghai Xushuo Biotechnology Co., Ltd. (Shanghai, China); bisphenol F (BPF), Bide Pharmatech Ltd. (Guizhou, China); styrene, benzoyl peroxide (BPO), toluene, chloroform, liquid paraffin, gelatin, methanol, acetic acid and ethanol, Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China); all the above reagents were analytically pure. Strata-X, Strata-X-CW, Strata-X-C and Strata-X-A, Phenomenex (Tianjin, China).
THZ-82A Constant Temperature Oscillator, Jintan Chengdong Xinrui Instrument Factory (Jiangsu, China); DHG type vacuum drying oven, Shangyu Huyue Equipment Factory (Zhejiang, China); FTIR-1500 Fourier transform infrared spectrometer (FTIR), Mettler Toledo Instruments Co., Ltd (Shanghai, China); Evo18 field emission high resolution scanning electron microscope (SEM), Zeiss AG, (Shanghai, China); NOVA 3200E specific surface area analyzer, Kantakmer Instrument Trading Co., Ltd. (Shanghai, China); LC-10AT high performance liquid chromatograph, Shimadzu Co., Ltd (Shanghai, China); B. V.Epyrean X-ray powder diffractometer (XRD), Panaco, Netherlands.
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8

Fluorinated Methacrylate Polymer Synthesis

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Trifluoroethyl methacrylate (G03, 96+%), hexafluorobutyl methacrylate (G02, 96+%) and dodecafluoroheptyl methacrylate (G04, 96+%), were supplied by XEOGIA Fluorine-Silicon Chemical Co., Ltd. Isobornyl methacrylate (IBOMA, 85%) was purchased from Sahn Chemical Technology Co., Ltd. Styrene (St, 99.5+%), acrylic acid (AA, 99.5+%) and benzoyl peroxide (BPO, 99.7+%) were from Sinopharm Chemical Reagent Co., Ltd. 3-(Trimethoxysilyl)propyl methacrylate (KH-570, 98%) was provided by Jining Huakai Resin Co., Ltd. Dimethyl carbonate (DMC, 99+%) and ethanol (EtOH, 99.5+%) were supplied by Shanghai Gaoyun Chemical Co., Ltd. All reagents and solvent were used as received without further purification. The old bricks with dimensions 22 cm × 10 cm × 4 cm were obtained from Hongcun village, Yixian county, Anhui province, 300 years ago and cut into 5 cm × 5 cm × 1 cm for water vapor permeability test, 1 cm × 1 cm × 2 cm for the water contact angle measurement, water absorption by total immersion, freeze–thaw cycles test and soluble salts aging test.
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9

Recombinant ω-Transaminase Immobilization

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The recombinant ω-TA (E.C. 2.6.1.1) originated from Mycobacterium vanbaalenii was purchased from Sigma–Aldrich. Polyvinyl alcohol (PVA, 1750 ± 50), ferric chloride hexahydrate (FeCl3·6H2O), ferrous chloride tetrahydrate (FeCl2·4H2O), concentrated hydrochloric acid, 25% glutaraldehyde, n-butanol, benzoyl peroxide, dodecyl trimethyl ammonium chloride, Tween 80, Coomassie Brilliant Blue G250 and bovine serum albumin were obtained from Sinopharm. Pyridoxal-5′-phosphate (PLP), R-α-methylbenzylamine (R-α-MBA) were supplied by Aladdin. Edible oil was purchased from a local firm. All other reagents used were of analytic grade.
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10

Synthesis and Characterization of PA66 Particles

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PA66 particles (industrial grade) were obtained from DuPont Corporation. Sodium hypophosphite (with analytical purity) was purchased from Shanghai Aibe Chemical Reagent Co., Ltd (Shanghai, China). Glacial acetic acid, cyclohexene, benzoyl peroxide, sodium hydroxide, zinc sulfate, sulfuric acid, aluminum sulfate, and magnesium sulfate (with analytical purity) were acquired from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
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